RESUMO
The enzyme glucosyltransferase plays an important role in plaque formation and growth. Therefore, chemical inhibition of glucosyltransferase may become an effective method for plaque control. In this investigation we have evaluated the effects of some antiplaque substances (chlorhexidine, cetylpiridinium chloride, iodine, sodium fluoride and sodium dodecyl sulfate) on glucosyltransferase activity. Our results revealed that iodine was the most effective inhibitor. Based on in vitro glucosyltransferase inhibition we may suggest that topical iodine could be an auxiliary method for plaque control.
Assuntos
Placa Dentária/enzimologia , Glucosiltransferases/antagonistas & inibidores , Criança , Placa Dentária/prevenção & controle , Glucosiltransferases/isolamento & purificação , HumanosRESUMO
A simplified method for the partial purification and enzyme assay of glucosyltransferase from human dental plaque is described. The enzyme obtained has a reasonable specific activity for the assay of soluble and insoluble polysaccharides synthesis. This method may be useful for nonacquainted people with enzymological methods and of great utility in the in vitro evaluation of antiplaque substances.
