1.
Biochim Biophys Acta
; 1421(1): 1-4, 1999 Sep 21.
Artigo
em Inglês
| MEDLINE
| ID: mdl-10561466
RESUMO
The presence of EDTA in the suspending buffer can induce the formation of multilayer structures from a mixture of the cationic lipid 3beta[N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol and the zwitterionic 'helper' lipid 1, 2-dimyristoyl-sn-glycero-3-phosphocholine with DNA. The resulting structures consist of stacks of alternating sheets of lipid bilayer with intercalated DNA. In the absence of EDTA, only a single layer of DNA adsorbs to the lipid membrane. The buffer composition therefore influences the morphology of the lipid-aggregate/DNA assembly, which was not known to date.