Chromosomal localization of the telomeric (TTAGGG)n sequence in four species of Armadillo (Dasypodidae) from Argentina: an approach to explaining karyotype evolution in the Xenarthra.

The distribution of the vertebrate telomeric sequence (TTAGGG)(n) in four species of armadillos (Dasypodidae, Xenarthra), i.e. Chaetophractus villosus (2n = 60), Chaetophractus vellerosus (2n = 62), Dasypus hybridus (2n = 64) and Zaedyus pichiy (2n = 62) was examined by FISH with a peptide nucleic acid (PNA) probe. Besides the expected telomeric hybridization, interstitial (centromeric) locations of the (TTAGGG)n sequence were observed in one chromosome pair of Chaetophractus vellerosus and Zaedyus pichiy, suggesting chromosome fusion of ancestral chromosomes occurring during the evolution of Dasypodidae. In addition, all the species analysed showed one to four apparently telocentric chromosomes, exhibiting only two telomeric signals. However, the immunodetection study of kinetochore proteins on synaptonemal complex spreads from C. villosus showed that the apparently telocentric chromosomes have a tiny short arm that can be resolved only in the more elongated pachytene bivalents. This finding suggests that none of the species of armadillos possess true telocentric chromosomes. Our present results support a reduction in the diploid number by fusion of acrocentrics with loss of chromosome material as a tendency in Dasypodidae.

Evaluation of the Radiation Exposures of Chernobyl "Liquidators": Exploratory Studies of a Sample of Immigrants to Israel.

Workers who were involved in the clean-up and recovery operation after the Chernobyl disaster ("liquidators") numbered about 800,000. Estimates of their exposures are highly relevant to evaluation of possible effects on their health. While some exposure measurements were made, they were also used for job assignments and terminations. The earliest and probably greatest exposure preceded adequate monitoring. By means of exploratory studies, the authors evaluated an exposure-estimating system proposed by a WHO consultation team in St. Petersburg. An index is derived based on recorded or reported 1) calendar time of initiating work, 2) location near or distant from the reactor, 3) duration of exposure, 4) type of work, 5) post-exposure symptoms, 6) blood count decreases, and 7) use of protective equipment. Data were obtained by interviews of liquidators who presented at a Beer Sheva clinic during evaluation of Cs-137 body burden. Of 75 liquidators, four had no data, one had only one item, six had two, 11 had three, 210 had four, 20 had five, 11 had six, and one had seven items. Only 19 had dose estimates. There was no trend in mean scores/item with increase in the number of items, indicating lack of information bias. Measured values, when available, were consistent with the scores. Clastogenic scores were determined for 26 of these subjects; the correlation with exposure indexes was 0.02, but for those with "positive" clastogenic tests (more than six abnormal divisions per 100), the correlation with the scores was 0.44. No such relationship was found for the persons with elevated Cs-137 tests. Glycophorin A antigen tests discriminated between liquidators and others, but, in this small study, did not correlate with the indexes.

Thyroid-stimulating hormone levels in children from Chernobyl.

This study assesses parameters of thyroid function in persons who resided in Ukraine, Belarus, and southern Russia and exposed at 0 to 16 years of age to radioiodine contamination from the Chernobyl accident. Six to eight years after the accident a group of 300 young people who had immigrated to Israel were interviewed, underwent physical and ultrasound thyroid examination, and had their serum tested for thyroid-stimulating hormones (TSH), thyroid hormones, thyroglobulin, and antithyroid antibodies. Comparative groups came from areas with high (>1 Ci/km2) or low (< 1 Ci/km2) 137Cs ground contamination. Girls from high contamination areas, when compared to girls from areas with low ground contamination, showed significant upward shifts in levels of serum TSH (p = 0.023) although remaining within normal limits. Boys showed no significant differences. There was no evidence for differences in thyroid size or nodularity between the two groups of girls. A working hypothesis is proposed by which the shift in TSH levels in girls from high radiocontamination areas was associated with subclinical radiation damage from environmental radioiodine at the time of the accident.

Lessons learned from the study of immigrants to Israel from areas of Russia, Belarus, and Ukraine contaminated by the Chernobyl accident.

During the past 6 years, immigration to Israel of 700,000 persons from the former Soviet Union (FSU) included about 140,000 from radiocontaminated regions of Belarus, Ukraine, and Russia near Chernobyl. In Beer Sheva, a major center for immigrant absorption in Israel, a primary objective was to evaluate their health status and to refer them for care. 137Cs levels in 1228 men, women, and children were measured with a portable whole-body counter. Whole-body counts showed clear correlation with the degree of 137Cs ground contamination in previous regions of residence. The population could thus be sub-divided according to degree of exposure, based on previous regions of residence. The thyroid status of 300 local immigrant children was evaluated because of the increased risk of childhood thyroid cancer in the regions from which they came. This group was subdivided into comparative groups of children who came from less and more contaminated areas according to the International Atomic Energy Agency soil 137Cs contamination maps. Enlarged thyroids were found in about 40% of both groups. One 12-year-old girl from Gomel had a malignant papillary carcinoma. Thyroid-stimulating hormone levels, though within normal limits, were significantly greater (p < 0.02) for girls from high exposure regions. Liquidators showed significant increases in serum clastogenic factor and in the number of circulating glycophorin A-mutated red cells. In studies of over 700 people from both radiocontaminated and unaffected regions of the FSU, evidence for posttraumatic stress disorder was found more frequently in persons coming from the more contaminated areas.

Health effects in a casual sample of immigrants to Israel from areas contaminated by the Chernobyl explosion.

We analyzed questionnaire and physician examination data for 1560 new immigrants from the former USSR divided into three groups by potential exposure to Chernobyl radiation. Two groups were chosen according to soil contamination by cesium-137 at former residences, as confirmed by our findings in a 137Cs body burden study. The third group consisted of "liquidators," persons who worked at the Chernobyl site after the disaster. Liquidators had greater self-reported incidences of symptoms commonly accepted as acute effects of radiation exposure, increases in prevalence of hypertension, and more health complaints. Excesses of bronchial asthma and health complaints were reported in children from the more exposed communities. Asthma prevalence in children potentially exposed in utero appears to be increased eightfold. Older adults from more exposed areas had more hypertension as assessed by history and measurements. These findings suggest the possible association of radiation exposure with several nonmalignant effects.

Antibodies to spotted fever group rickettsiae and Coxiella burnetii among domestic animals in southern Croatia.

The sera of dogs, goats, sheep and cattle (total 153) from the Kastelas' bay area (middle part of the eastern coast of the Adriatic Sea) were tested for antibodies against Rickettsia conorii and Coxiella burnetii by a complement fixation test. The overall percentages of positive sera among the tested animals were 23.9% for R. conorii and 16.4% for C. burnetii. The results show that animals in this area are clearly being exposed to spotted fever group rickettsiae as well as to C. burnetii. For comparison, six sheep sera from the flock, living in known Q fever focus in hinterland area and linked with outbreak of Q fever among owners were tested. Antibodies to C. burnetii but not for R. conorii were present in all six sera.