THE LEVEL OF REACTIVE OXYGEN SPECIES AS A MARKER OF ASTHMA SEVERITY IN CHILDREN.

OBJECTIVE: The aim of the research was to assess the reactive oxygen species (ROS) levels in granulocytes of patients with asthma. PATIENTS AND METHODS: Materials and methods: The study involved 35 children aged 5 to 17 years. 26 children with persistent asthma, partially controlled course in the period of exacerbation were divided into groups: 1 group - mild asthma (n = 12), group 2 - moderate asthma (n = 7) group 3 - severe asthma (n = 7) and control group included almost healthy children (n = 9). ROS levels in granulocytes were evaluated using BD FACSDiva™. The spirographic complex was used to assess the function of external respiration. RESULTS: Results: The level of ROS in granulocytes of patients with severe asthma was significantly reduced compared with children in the control group and patients with mild and moderate asthma (p1-3 = 0.0003, p2-3 = 0.0017, p c-3 = 0.0150). The concentration of ROS in granulocytes ≤ 285 a.u. was prognostically significant with high specificity and sensitivity with severe asthma. CONCLUSION: Conclusions: The concentration of ROS levels in neutrophils in patients with severe asthma probably reflected the suppression of their products, which suggests the depletion of the reserve capacity of neutrophils. Decreased concentrations of reactive oxygen species in children with asthma can be considered as a possible marker of asthma severity.

THE ROLE OF POLYMORPHISMS OF MATRIX METALLOPROTEINASES' POLYMORPHISMS 1 AND 12 IN THE FORMATION OF WHEEZING SYNDROME AMONG CHILDREN WITH RECURRENT BRONCHITIS.

OBJECTIVE: The aim: Matrix metalloproteinases (MMP) play an important role in the architecture and remodeling of the lungs. There are 2 gene families of MMP among significantly different genes - MMP-1 and MMP-12, which are closely related to the pathophysiological processes of allergic inflammation, damage and restoration of tissues and the body's defense against pathogens. PATIENTS AND METHODS: Materials and methods: 70 examined children were divided into 2 groups: 37 children who had acute recurrent bronchitis complicated by wheezing syndrome, the comparison group included 33 children with acute bronchitis. The determination of gene polymorphism was carried out using ELISA analysis. RESULTS: Results: In the dominant model, carriers of the 2G allele genotypes had 3,45 times lower risk of wheezing syndrome compared with patients with the 1G/1G genotype (OR = 3,45, 95% CI: 1,07-11.15, p<0,05). In the dominant model, carriers of G-allele genotypes had a 4,2-fold lower risk of wheezing syndrome compared with patients with the AA genotype (OR = 4,2; 95% CI (CI) = 1,09- 16,09; p <0,05). CONCLUSION: Conclusions: Polymorphism rs1799750 in the MMP-1 gene increases the risk of developing the wheezing syndrome among children with acute recurrent bronchitis in 3,5 times. The rs2276109 polymorphism in the MMP-12 gene reduces the risk of wheezing syndrome by 4,2 times among children with acute recurrent bronchitis.