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1.
PLoS One ; 12(3): e0172718, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28249043

RESUMO

In severe falciparum malaria cytoadherence of parasitised red blood cells (PRBCs) to vascular endothelium (causing sequestration) and to uninfected red cells (causing rosette formation) contribute to microcirculatory flow obstruction in vital organs. Heparin can reverse the underlying ligand-receptor interactions, but may increase the bleeding risks. As a heparin-derived polysaccharide, sevuparin has been designed to retain anti-adhesive properties, while the antithrombin-binding domains have been eliminated, substantially diminishing its anticoagulant activity. Sevuparin has been evaluated recently in patients with uncomplicated falciparum malaria, and is currently investigated in a clinical trial for sickle cell disease. The effects of sevuparin on rosette formation and cytoadherence of Plasmodium falciparum isolates from Thailand were investigated. Trophozoite stages of P. falciparum-infected RBCs (Pf-iRBCs) were cultured from 49 patients with malaria. Pf-iRBCs were treated with sevuparin at 37°C and assessed in rosetting and in cytoadhesion assays with human dermal microvascular endothelial cells (HDMECs) under static and flow conditions. The proportion of Pf-iRBCs forming rosettes ranged from 6.5% to 26.0% (median = 12.2%). Rosetting was dose dependently disrupted by sevuparin (50% disruption by 250 µg/mL). Overall 57% of P. falciparum isolates bound to HDMECs under static conditions; median (interquartile range) Pf-iRBC binding was 8.5 (3.0-38.0) Pf-iRBCs/1000 HDMECs. Sevuparin in concentrations ≥ 100 µg/mL inhibited cytoadherence. Sevuparin disrupts P. falciparum rosette formation in a dose dependent manner and inhibits cytoadherence to endothelial cells. The data support assessment of sevuparin as an adjunctive treatment to the standard therapy in severe falciparum malaria.


Assuntos
Células Endoteliais/metabolismo , Eritrócitos/parasitologia , Heparina/análogos & derivados , Malária Falciparum/metabolismo , Plasmodium falciparum/metabolismo , Trofozoítos/metabolismo , Adolescente , Adulto , Idoso , Adesão Celular/efeitos dos fármacos , Células Cultivadas , Eritrócitos/metabolismo , Feminino , Heparina/farmacologia , Humanos , Masculino , Pessoa de Meia-Idade , Formação de Roseta
2.
Malar J ; 14: 389, 2015 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-26437894

RESUMO

BACKGROUND: The pathogenesis of pulmonary oedema (PE) in patients with severe malaria is still unclear. It has been hypothesized that lung injury depends, in addition to microvascular obstruction, on an increased pulmonary capillary pressure and altered alveolar-capillary membrane permeability, causing pulmonary fluid accumulation. METHODS: This study compared the histopathological features of lung injury in Southeast Asian patients (n = 43) who died from severe Plasmodium falciparum malaria, and correlated these with clinical history in groups with or without PE. To investigate the expression of mediators that may influence fluid accumulation in PE, immunohistochemistry and image analysis were performed on controls and sub-sets of patient with or without PE. RESULTS: The expression of leukocyte sub-set antigens, bronchial interleukin (IL)-33, γ-epithelium sodium channel (ENaC), aquaporin (AQP)-1 and -5, and control cytokeratin staining was quantified in the lung tissue of severe malaria patients. Bronchial IL-33 expression was significantly increased in severe malaria patients with PE. Malaria patients with shock showed significantly increased bronchial IL-33 compare to other clinical manifestations. Bronchial IL-33 levels were positively correlated with CD68+ monocyte and elastase + neutrophil, septal congestion and hyaline membrane formation. Moreover, the expression of both vascular smooth muscle cell (VSMC) and bronchial γ-ENaC significantly decreased in severe malaria patients with PE. Both VSMC and bronchial γ-ENaC were negatively correlated with the degree of parasitized erythrocyte sequestration, alveolar thickness, alveolar expansion score, septal congestion score, and malarial pigment score. In contrast AQP-1 and -5 and pan cytokeratin levels were similar between groups. CONCLUSIONS: The results suggest that IL-33 may play a role in lung injury during severe malaria and lead to PE. Both VSMC and bronchial γ-ENaC downregulation may explain pulmonary fluid disturbances and participate in PE pathogenesis in severe malaria patients.


Assuntos
Canais Epiteliais de Sódio/metabolismo , Interleucina-33/metabolismo , Malária Falciparum/complicações , Malária Falciparum/patologia , Edema Pulmonar/etiologia , Edema Pulmonar/patologia , Adolescente , Adulto , Sudeste Asiático , Criança , Pré-Escolar , Feminino , Histocitoquímica , Humanos , Processamento de Imagem Assistida por Computador , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Adulto Jovem
3.
Int J Clin Exp Pathol ; 7(5): 2056-67, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24966914

RESUMO

The mechanisms leading to cerebral malaria (CM) are not completely understood. Brain edema has been suggested as having an important role in experimental CM. In this study, CBA/CaH mice were infected with Plasmodium berghei ANKA blood-stage and when typical symptoms of CM developed on day 7, brain tissues were processed for electron-microscopic and immunohistochemical studies. The study demonstrated ultrastructural hallmarks of cerebral edema by perivascular edema and astroglial dilatation confirming existing evidence of vasogenic and cytogenic edema. This correlates closely with the clinical features of CM. An adaptive response of astrocytic activity, represented by increasing glial fibrillary acidic protein (GFAP) expression in the perivascular area and increasing numbers of large astrocyte clusters were predominately found in the CM mice. The presence of multivesicular and lamellar bodies indicates the severity of cerebral damage in experimental CM. Congestion of the microvessels with occluded white blood cells (WBCs), parasitized red blood cells (PRBCs) and platelets is also a crucial covariate role for CM pathogenesis.


Assuntos
Edema Encefálico/metabolismo , Edema Encefálico/patologia , Encéfalo/irrigação sanguínea , Encéfalo/metabolismo , Encéfalo/ultraestrutura , Proteína Glial Fibrilar Ácida/metabolismo , Malária Cerebral/metabolismo , Malária Cerebral/patologia , Microscopia Eletrônica de Transmissão , Animais , Astrócitos/metabolismo , Astrócitos/parasitologia , Astrócitos/ultraestrutura , Encéfalo/parasitologia , Edema Encefálico/parasitologia , Dilatação Patológica , Modelos Animais de Doenças , Feminino , Imuno-Histoquímica , Malária Cerebral/parasitologia , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos CBA , Microvasos/metabolismo , Microvasos/parasitologia , Microvasos/ultraestrutura , Plasmodium berghei/patogenicidade , Fatores de Tempo
4.
Malar J ; 13: 4, 2014 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-24386908

RESUMO

BACKGROUND: Malaria in pregnancy remains a major health problem. Placental malaria infection may cause pathophysiological changes in pregnancy and result in morphological changes to placental villi. Quantitative histomorphological image analysis of placental biopsies was performed to compare placental villous architecture between active or treated placental malaria cases and controls. METHODS: A total of 67 placentas were studied from three clinical groups: control patients who did not have malaria (n = 27), active (n = 14) and treated (n=26) malaria cases, including both Plasmodium falciparum and Plasmodium vivax infections. Image analysis of histological placental sections was performed using ImageJ software to measure the number and size (area) of terminal villi, perimeter measurement per villus and total perimeter per unit area, and number of capillaries per villus (vascularity). Histological features of placental malaria were scored and these results were correlated with malaria status and clinical outcomes. RESULTS: Villous size correlated with vascularity (p <0.0001) but was inversely correlated with observed villi per unit area, (p = 0.0001). Significantly greater villous area and vascularity was observed in UK controls. Indices of histological malaria infection were significantly greater in active versus treated malaria cases. Active placental malaria cases showed significantly smaller villous area (p <0.0084), vascularity (p <0.0139) and perimeter (p <0.0006) than treated malaria cases or controls, but significantly more villi per unit area (p <0.0001). Villous size in treated malaria cases was significantly larger than active placental malaria cases (p <0.001) and similar to controls. There was a significant relationship between villous number and anaemia at the time of infection (p <0.0034), but not placental weight, birth weight or gestational age at delivery. No differences were found between histology or villous morphology comparing infections with P. falciparum or P. vivax. CONCLUSIONS: These results imply that villous size, perimeter and vascularity are acutely decreased during active placental malaria, decreasing the surface area available for gas exchange per villus. However the increased number of villi per unit area offsets this change and persists after treatment. Histopathological and villous architectural changes may be reversed by early detection and appropriate anti-malarial treatment.


Assuntos
Antimaláricos/uso terapêutico , Malária Falciparum/patologia , Malária Vivax/patologia , Placenta/patologia , Complicações Infecciosas na Gravidez/patologia , Adolescente , Adulto , Feminino , Humanos , Malária Falciparum/parasitologia , Malária Vivax/parasitologia , Placenta/parasitologia , Plasmodium falciparum/fisiologia , Plasmodium vivax/fisiologia , Gravidez , Tailândia , Adulto Jovem
5.
J Toxicol Pathol ; 26(3): 283-91, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24155561

RESUMO

A one-time oral gavage can be enough to cause of alveologenic edema with higher expression of AQP-1 and -4 than that with repeated-dose oral gavage, which caused both profound perivascular edema and hydrostatic pressure edema, while AQP-5 was similarly expressed. The alteration of AQPs expression was probably related to alveolar fluid clearance across the alveolar and bronchiolar epithelium in different stages of lung injury. The results clarified the type of lung edema in acute and sub-chronic toxicity studies without treatment related effect of tested material. The pathogenesis of pulmonary edema due to oral gavage toxicological study is associated with the cellular immune response to the reflux materials. Mast cell and leukocyte accumulation may contribute to increase vascular permeability leading to permeability edema. The increase in alveolar septum epithelium, perivascular and peribronchial cuffing, accumulation alveolar lipid containing macrophage and medial hyperplasia of the pulmonary artery might have been caused to increase airway resistance, which resulted in hydrostatic pressure edema.

6.
Malar J ; 11: 191, 2012 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-22682094

RESUMO

BACKGROUND: Malaria parasites and their products can activate a specific immune response by stimulating cytokine production in the host's immune cells. Transcription nuclear factor kappa B (NF-κB) is an important regulator for the control of many pro-inflammatory genes, such as interleukin-1 (IL-1) and tumor necrosis factor (TNF). The activation and expression of NF-κB p65 in peripheral blood mononuclear cells (PBMCs) of malaria patients were investigated and correlated with the levels of IL-10 and TNF to study the nature of NF-κB p65 and its linkage to inflammatory cytokines. METHODS: The sample group comprised 33 patients admitted with malaria caused by Plasmodium vivax (n = 11), uncomplicated Plasmodium falciparum (n = 11), and complicated Plasmodium falciparum (n = 11). Peripheral blood was collected at admission and on day 7 for PBMC isolation. Healthy subjects were used as a control group. The expressions of NF-κB p65 in the PBMCs from malaria patients and the plasma levels of IL-10 and TNF were measured by using enzyme-linked immunosorbent assay (ELISA). The immunofluorescence technique was used to determine NF-κB nuclear translocation. RESULTS: At admission, patients with P. vivax and uncomplicated P. falciparum had significantly elevated phospho-NF-κB p65 levels in the PBMCs compared with those of healthy controls. However, patients with complicated P. falciparum malaria had decreased levels of phospho-NF-κB p65. On day 7 post-treatment, significantly increased phospho-NF-κB p65 was found in the PBMCs of patients with complicated P. falciparum, compared with healthy controls. The plasma level of IL-10 was elevated in day 0 in patients with complicated P. falciparum malaria and was found to be negatively correlated with phospho-NF-κB p65 level (rs = -0.630, p = 0.038). However, there was no correlation between phospho-NF-κB p65 expression and TNF level in patients with complicated P. falciparum malaria. CONCLUSIONS: This is the first report demonstrating alterations in NF-κB p65 activity in the PBMCs of malaria patients. The altered lower features of NF-κB p65 in the PBMCs of patients with complicated P. falciparum at admission could be due to a suppressive effect of high IL-10 associated with complicated P. falciparum malaria.


Assuntos
Sangue/imunologia , Leucócitos Mononucleares/imunologia , Malária Falciparum/imunologia , Malária Vivax/imunologia , Fator de Transcrição RelA/biossíntese , Adolescente , Adulto , Ensaio de Imunoadsorção Enzimática , Feminino , Imunofluorescência , Humanos , Interleucina-10/sangue , Masculino , Pessoa de Meia-Idade , Fator de Necrose Tumoral alfa/sangue , Adulto Jovem
7.
Artigo em Inglês | MEDLINE | ID: mdl-23431813

RESUMO

We examined the ultrastructure of Plasmodium malariae-infected erythrocytes in peripheral blood and tissue biopsies of the liver, stomach, and duodenum from three patients infected with P. malariae. Ultrastructural features of P. malariae-infected erythrocytes in peripheral blood appear similar to those described previously. The surface membranes of P. malariae-infected erythrocytes had numerous knobs, as seen in P. falciparum-infected erythrocytes. There was no evidence of P. malariae-infected erythrocytes in the microvessels of the organs. This finding suggests the presence of knobs on P. malariae-infected erythrocytes is not associated with the attachment of P. malariae-infected erythrocytes to vascular endothelium and may be the reason for the mild symptoms of malariae malaria. The failure to find P. malariae-infected erythrocytes in the tissue biopsies using electron microscopy may be due to low parasitemia. More cases with higher parasitemia need to be studied to confirm these findings.


Assuntos
Duodeno/parasitologia , Eritrócitos/ultraestrutura , Fígado/parasitologia , Malária/diagnóstico , Parasitemia/parasitologia , Plasmodium malariae/ultraestrutura , Estômago/parasitologia , Adolescente , Adulto , Eritrócitos/parasitologia , Feminino , Humanos , Malária/parasitologia , Masculino , Microscopia Eletrônica
8.
Malar J ; 10: 267, 2011 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-21923924

RESUMO

BACKGROUND: The role of brain oedema in the pathophysiology of cerebral malaria is controversial. Coma associated with severe Plasmodium falciparum malaria is multifactorial, but associated with histological evidence of parasitized erythrocyte sequestration and resultant microvascular congestion in cerebral vessels. To determine whether these changes cause breakdown of the blood-brain barrier and resultant perivascular or parenchymal cerebral oedema, histology, immunohistochemistry and image analysis were used to define the prevalence of histological patterns of oedema and the expression of specific molecular pathways involved in water balance in the brain in adults with fatal falciparum malaria. METHODS: The brains of 20 adult Vietnamese patients who died of severe malaria were examined for evidence of disrupted vascular integrity. Immunohistochemistry and image analysis was performed on brainstem sections for activation of the vascular endothelial growth factor (VEGF) receptor 2 and expression of the aquaporin 4 (AQP4) water channel protein. Fibrinogen immunostaining was assessed as evidence of blood-brain barrier leakage and perivascular oedema formation. Correlations were performed with clinical, biochemical and neuropathological parameters of severe malaria infection. RESULTS: The presence of oedema, plasma protein leakage and evidence of VEGF signalling were heterogeneous in fatal falciparum malaria and did not correlate with pre-mortem coma. Differences in vascular integrity were observed between brain regions with the greatest prevalence of disruption in the brainstem, compared to the cortex or midbrain. There was a statistically non-significant trend towards higher AQP4 staining in the brainstem of cases that presented with coma (P = .02). CONCLUSIONS: Histological evidence of cerebral oedema or immunohistochemical evidence of localised loss of vascular integrity did not correlate with the occurrence of pre-mortem coma in adults with fatal falciparum malaria. Enhanced expression of AQP4 water channels in the brainstem may, therefore, reflect a mix of both neuropathological or attempted neuroprotective responses to oedema formation.


Assuntos
Coma/etiologia , Coma/fisiopatologia , Malária Cerebral/complicações , Plasmodium falciparum/patogenicidade , Adulto , Idoso , Aquaporina 4/análise , Barreira Hematoencefálica/patologia , Barreira Hematoencefálica/fisiopatologia , Edema Encefálico/patologia , Edema Encefálico/fisiopatologia , Feminino , Fibrinogênio/análise , Histocitoquímica , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Neuroimagem , Fator A de Crescimento do Endotélio Vascular/análise , Adulto Jovem
9.
Ultrastruct Pathol ; 35(5): 214-25, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21910567

RESUMO

Ultrastructural changes to P. falciparum-infected red blood cells were examined in vitro after treatment with antimalarial drugs. Artesunate had the most rapid parasitocidal effect. All three drugs caused structural changes within the parasite, including dilatation of the parasitophorus vacuole membrane, depletion of ribosomes, mitochondrial swelling, and decreased formation of hemozoin crystals. The structure of surface knobs and Maurer's clefts were similar to controls but reduced in number. Only depletion of free ribosomes correlated with antimalarial drug exposure. Drug treatment decreased movement of hemozoin granules within parasites on real-time microscopy, before recognizable morphological changes of parasite death.


Assuntos
Eritrócitos/parasitologia , Malária Falciparum , Plasmodium falciparum/ultraestrutura , Antimaláricos/farmacologia , Artemisininas/farmacologia , Artesunato , Células Cultivadas , Eritrócitos/ultraestrutura , Interações Hospedeiro-Parasita , Malária Falciparum/tratamento farmacológico , Malária Falciparum/patologia , Microscopia Eletrônica de Transmissão , Plasmodium falciparum/efeitos dos fármacos , Plasmodium falciparum/fisiologia , Quinina/farmacologia , Quinolinas/farmacologia , Ribossomos/efeitos dos fármacos , Ribossomos/ultraestrutura , Trofozoítos/efeitos dos fármacos , Trofozoítos/fisiologia
10.
Int J Clin Exp Pathol ; 4(6): 566-74, 2011 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-21904632

RESUMO

The pathogenic mechanisms underlying the occurrence of cerebral malaria (CM) are still incompletely understood but, clearly, cerebral complications may result from concomitant microvessel obstruction and inflammation. The extent to which brain edema contributes to pathology has not been investigated. Using the model of P. berghei ANKA infection, we compared brain microvessel morphology of CM-susceptible and CM-resistant mice. By quantitative planimetry, we provide evidence that CM is characterized by enlarged perivascular spaces (PVS). We show a dramatic aquaporin 4 (AQP4) upregulation, selectively at the level of astrocytic foot processes, in both CM and non-CM disease, but significantly more pronounced in mice with malarial-induced neurological syndrome. This suggests that a threshold of AQP4 expression is needed to lead to neurovascular pathology, a view that is supported by significantly higher levels in mice with clinically overt CM. Numbers of intravascular leukocytes significantly correlated with both PVS enlargement and AQP4 overexpression. Thus, brain edema could be a contributing factor in CM pathogenesis and AQP4, specifically in its astrocytic location, a key molecule in this mechanism. Since experimental CM is associated with substantial brain edema, it models paediatric CM better than the adult syndrome and it is tempting to evaluate AQP4 in the former context. If AQP4 changes are confirmed in human CM, it may represent a novel target for therapeutic intervention.


Assuntos
Aquaporina 4/metabolismo , Astrócitos/patologia , Edema Encefálico/patologia , Malária Cerebral/patologia , Animais , Astrócitos/metabolismo , Astrócitos/parasitologia , Biomarcadores/metabolismo , Encéfalo/irrigação sanguínea , Encéfalo/metabolismo , Encéfalo/parasitologia , Edema Encefálico/metabolismo , Edema Encefálico/parasitologia , Modelos Animais de Doenças , Feminino , Leucócitos/metabolismo , Leucócitos/patologia , Malária Cerebral/complicações , Malária Cerebral/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos CBA , Plasmodium berghei , Especificidade da Espécie , Regulação para Cima
11.
Histopathology ; 57(2): 282-94, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20716170

RESUMO

AIMS: Pathological or neuroprotective mechanisms in the brain in severe malaria may arise from microvascular obstruction with malaria-parasitized erythrocytes. This study aimed to investigate the role of hypoxia and induction of the vascular endothelial growth factor (VEGF) pathway in the neuropathophysiology of severe malaria. METHODS AND RESULTS: Immunohistochemistry was performed on post mortem brain tissue sections from 20 cases of severe malaria and examined for the expression of transcriptional regulators of VEGF [hypoxia-inducible factor-1 alpha (HIF-1alpha), HIF-2alpha], DEC-1, VEGF, VEGF receptors 1 and 2, and the activated, phosphorylated VEGF receptor 2 (pKDR). HIFs showed limited protein expression and/or translocation to cell nuclei in severe malaria, but DEC-1, which is more stable and regulated by HIF-1alpha, was observed. There was heterogeneous expression of VEGF and its receptors in severe malaria and non-malarial disease controls. pKDR expression on vessels was greater in malaria cases than in controls but did not correlate with parasite sequestration. VEGF uptake by malaria parasites was observed. CONCLUSIONS: VEGF and its receptor expression levels in severe malaria reflect a non-specific response to severe systemic disease. Potential manipulation of events at the vasculature by the parasite requires further investigation.


Assuntos
Encéfalo/metabolismo , Malária Cerebral/metabolismo , Malária Falciparum/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo , Adulto , Encéfalo/irrigação sanguínea , Encéfalo/parasitologia , Encéfalo/patologia , Estudos de Casos e Controles , Humanos , Imuno-Histoquímica , Malária Cerebral/parasitologia , Malária Cerebral/patologia , Malária Falciparum/parasitologia , Malária Falciparum/patologia , Microvasos/parasitologia , Fatores de Transcrição/metabolismo , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/metabolismo
12.
Asian Pac J Allergy Immunol ; 26(1): 37-45, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18595528

RESUMO

Plasmodium falciparum, the protozoan parasite responsible for severe malaria infection, undergoes a complex life cycle. Infected red blood cells (iRBC) sequester in host cerebral microvessels, which underlies the pathology of cerebral malaria. Using immunohistochemistry on post mortem brain samples, we demonstrated positive staining for vascular endothelial growth factor (VEGF) on iRBC. Confocal microscopy of cultured iRBC revealed accumulation of VEGF within the parasitophorous vacuole, expression of host VEGF-receptor 1 and activated VEGF-receptor 2 on the surface of iRBC, but no accumulation of VEGF receptors within the iRBC. Addition of VEGF to parasite cultures had a trophic effect on parasite growth and also partially rescued growth of drug treated parasites. Both these effects were abrogated when parasites were grown in serum-free medium, suggesting a requirement for soluble VEGF receptor. We conclude that P. falciparum iRBC can bind host VEGF-R on the erythrocyte membrane and accumulate host VEGF within the parasitophorous vacuole, which may have a trophic effect on parasite growth.


Assuntos
Eritrócitos/parasitologia , Malária Falciparum/parasitologia , Plasmodium falciparum/crescimento & desenvolvimento , Fator A de Crescimento do Endotélio Vascular/metabolismo , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Animais , Antimaláricos/farmacologia , Artemisininas/farmacologia , Artesunato , Células Cultivadas , Membrana Eritrocítica/metabolismo , Eritrócitos/metabolismo , Imunofluorescência , Humanos , Malária Falciparum/metabolismo , Microscopia Confocal , Plasmodium falciparum/efeitos dos fármacos , Fator A de Crescimento do Endotélio Vascular/farmacologia
13.
Trop Med Int Health ; 12(9): 1037-50, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17875015

RESUMO

OBJECTIVE: To use electron microscopy to examine the role of parasitized red blood cell (PRBC) sequestration in the pathogenesis of acute renal failure in severe falciparum malaria. METHODS: Ultrastructural pathological examination of renal tissues from Southeast Asian adults (n = 63) who died from severe falciparum malaria. Qualitative and quantitative determination of the major pathological features of disease, including PRBC and leukocyte sequestration. Clinico-pathological correlation with the pre-mortem clinical picture and peripheral parasite count. RESULTS: There was a high incidence of malaria-associated renal failure in this population (> 40%) and a correlation between this incidence, severe malarial anaemia and shock. Pathological features included PRBC sequestration in glomerular and tubulo-interstitial vessels, acute tubular damage and mild glomerular hypercellularity resulting from the accumulation of host monocytes within glomerular capillaries. No evidence for an immune complex mediated glomerulonephritis was found. There was a correlation between parasite sequestration in the kidney and pre-mortem renal failure, although overall levels of sequestration were relatively low. Levels of sequestration (Knob+ PRBC) were significantly higher in malaria-associated renal failure than in fatal cases without renal failure (P = 0.005). CONCLUSION: Malaria-associated renal failure is a common and serious complication of severe Plasmodium falciparum malaria in this population, associated with acute tubular injury rather than glomerulonephritis, and linked to localization of host monocytes in the kidney as well as sequestration of PRBCs.


Assuntos
Injúria Renal Aguda/mortalidade , Rim/ultraestrutura , Malária Falciparum/mortalidade , Injúria Renal Aguda/etiologia , Injúria Renal Aguda/patologia , Adolescente , Adulto , Idoso , Sudeste Asiático/epidemiologia , Criança , Pré-Escolar , Feminino , Humanos , Rim/patologia , Malária Falciparum/complicações , Malária Falciparum/patologia , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade
14.
Artigo em Inglês | MEDLINE | ID: mdl-17539270

RESUMO

Acute and chronic Toxoplasma infections were evaluated in mice using stage specific antibodies and immunocytochemistry. Mice with acute toxoplasmosis were less active, had erectile body hair and seldom took food or water resulting in weight loss. All mice died within 7 days post-inoculation. The immunohistochemical technique enhanced visualization of parasites allowing their distribution to be accurately followed. Following intraperitoneal infection, tachyzoites were initially identified on the surface of the liver and spleen. There was a rapid increase in the number of tachyzoites associated with invasion from the surrounding connective tissue into the organs with formation of inflammatory lesions in the liver. The focal inflammatory lesions showed increasing numbers of tachyzoites with the period post-inoculation. Similar increases in tachyzoites were observed for the spleen. In contrast, only a few individual tachyzoites were seen in the brain at the final time point. In chronic infections, the mice were asymptomatic but tissue cysts containing large numbers of bradyzoites were observed in all brains with the average number of 295 tissue cysts per half brain and the average cystic size of 46.02 +/- 5.08 microm. By histology and immunostaining, the tissue cysts were readily identifiable along with a mild inflammatory cell infiltration into the meninges and perivascular cuffing. Double immunocytochemical labelling confirmed the exclusive presence of tachyzoites during the acute phase and bradyzoites during the chronic phase.


Assuntos
Imuno-Histoquímica/métodos , Toxoplasma/imunologia , Toxoplasmose Animal/patologia , Doença Aguda , Animais , Anticorpos Antiprotozoários , Antígenos de Protozoários , Encéfalo/parasitologia , Doença Crônica , Feminino , Proteínas de Choque Térmico , Fígado/parasitologia , Masculino , Camundongos , Camundongos Endogâmicos ICR , Modelos Imunológicos , Proteínas de Protozoários , Baço/parasitologia , Toxoplasma/parasitologia , Toxoplasmose Animal/parasitologia
15.
Infect Immun ; 73(4): 1986-94, 2005 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15784539

RESUMO

The spleen is critical for host defense against pathogens, including Plasmodium falciparum. It has a dual role, not only removing aged or antigenically altered erythrocytes from the blood but also as the major lymphoid organ for blood-borne or systemic infections. The human malaria parasite P. falciparum replicates within erythrocytes during asexual blood stages and causes repeated infections that can be associated with severe disease. In spite of the crucial role of the spleen in the innate and acquired immune response to malaria, there is little information on the pathology of the spleen in human malaria. We performed a histological and quantitative immunohistochemical study of spleen sections from Vietnamese adults dying from severe falciparum malaria and compared the findings with the findings for spleen sections from control patients and patients dying from systemic bacterial sepsis. Here we report that the white pulp in the spleens of patients dying from malaria showed a marked architectural disorganization. We observed a marked dissolution of the marginal zones with relative loss of B cells. Furthermore, we found strong HLA-DR expression on sinusoidal lining cells but downregulation on cordal macrophages. P. falciparum infection results in alterations in splenic leukocytes, many of which are not seen in sepsis.


Assuntos
Malária Falciparum/imunologia , Baço/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Linfócitos B/patologia , Eritrócitos/patologia , Feminino , Antígenos HLA-DR/análise , Humanos , Imuno-Histoquímica , Imunofenotipagem , Contagem de Linfócitos , Malária Falciparum/patologia , Masculino , Pessoa de Meia-Idade , Células Mieloides/patologia , Príons/análise , Linfócitos T/patologia
16.
Southeast Asian J Trop Med Public Health ; 36(6): 1359-70, 2005 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-16610635

RESUMO

We performed a retrospective study of 25 patients who died of severe falciparum malaria in Thailand and Vietnam using electron microscopy. The aims of the study were: to determine if there was any significant association between parasitized red blood cells (PRBC) sequestered in liver and spleen and particular pre-mortem clinical complications, and to compare the degree of parasite load between the liver and spleen within the same patients. PRBC sequestrations in each organ were compared with the pre-mortem parasitemia, to calculate the sequestration index (S.I.). The S.I. showed that the degree of PRBC sequestration in the spleen was higher than the liver (S.I. median = 3.13, 0.87, respectively) (p < 0.05). The results of quantitative ultrastructural study showed a significantly high parasite load in the liver of patients with jaundice, hepatomegaly and liver enzyme elevation (p < 0.05). We found a significant correlation between PRBC sequestration in the liver and a high serum bilirubin level, a high aspartate aminotransferase (AST) level and an increase in the size of the liver (Spearman's correlation coefficient = 0.688, 0.572, 0.736, respectively). Furthermore, a higher parasite load was found in the liver of patients with acute renal failure (ARF) compared to patients without ARF (p < 0.05). These findings suggest that PRBC sequestration in the liver is quantitatively associated with pre-mortem hepatic dysfunction and renal impairment. There was no significant difference between splenomegaly and PRBC sequestration. The size of a palpable spleen was not correlated with parasite load in the spleen. When ultrastructural features were compared between the two reticuloendothelial organs, we found that the spleen had more PRBC and phagocytes than the liver. The spleen of non-cerebral malaria (NCM) patients had more phagocytes than cerebral malaria (CM) patients. This observation reveals that the spleen plays a major role in malaria parasite clearance, and is associated with host defence mechanisms against malaria.


Assuntos
Fígado/ultraestrutura , Malária Falciparum/mortalidade , Baço/ultraestrutura , Adolescente , Adulto , Idoso , Animais , Feminino , Humanos , Fígado/patologia , Malária Falciparum/patologia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Baço/patologia , Tailândia/epidemiologia , Vietnã
17.
Artigo em Inglês | MEDLINE | ID: mdl-16438223

RESUMO

The objective of this study was to investigate the ultrastructural changes of vascular pancreatic islets using a transmission electron microscopic technique. The major ultrastructural changes of microvessel in NOD mice are indicated by the swelling and vacuolization of the endothelial cell. Swollen cells are the first noticeable lesion of the cell response in reversible degeneration that is caused by the failure of homeostatic control. Loss in endothelial cell homeostasis is primarily a marker of endothelial dysfunction that plays a key role in the pathogenesis of diabetic vascular disease by losing the control of vascular tone. Diabetes also associates with an increased generation of oxygen-derived free radicals that may impair vasodilatation through the inactivation of vasodilators. In conclusion, consistent with a hypothesis that loss of the modulatory role of the endothelium may be a critical and initiating factor in the development of diabetic vascular disease, the ultrastructural changes in this study may indicate the first sign of endothelial dysfunction. This dysfunction correlates to the relationship between diabetes and reversible lesions of vessels in NOD mice, making for a better understanding of the pathophysiology of diabetic vascular disease to set the stage for further investigation to restore endothelial dysfunction in diabetes.


Assuntos
Diabetes Mellitus Tipo 1/fisiopatologia , Ilhotas Pancreáticas/ultraestrutura , Microcirculação/fisiopatologia , Animais , Células Endoteliais/patologia , Endotélio Vascular/fisiopatologia , Feminino , Ilhotas Pancreáticas/irrigação sanguínea , Camundongos , Camundongos Endogâmicos NOD , Microscopia Eletrônica , Medição de Risco , Fatores de Risco
18.
Acta Trop ; 89(3): 309-17, 2004 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-14744557

RESUMO

The pathophysiology of severe falciparum malaria is complex, but evidence is mounting that its central feature is the old concept of a mechanical microcirculatory obstruction. Autopsy studies, but also in vivo observations of the microcirculation, demonstrate variable obstruction of the microcirculation in severe malaria. The principal cause of this is cytoadherence to the vascular endothelium of erythrocytes containing the mature forms of the parasite, leading to sequestration and obstruction of small vessels. Besides, parasitized red cells become rigid, compromising their flow through capillaries whose lumen has been reduced by sequestered erythrocytes. Adhesive forces between infected red cells (auto-agglutination), between infected and uninfected red cells (rosetting) and between uninfected erythrocytes (aggregation) could further slow down microcirculatory flow. A more recent finding is that uninfected erythrocytes also become rigid in severe malaria. Reduction in the overall red cell deformability has a strong predictive value for a fatal outcome. Rigidity may be caused by oxidative damage to the red blood cell membrane by malaria pigment released at the moment of schizont rupture. Anti-oxidants, such as N-acetylcysteine can reverse this effect and are promising as adjunctive treatment in severe malaria.


Assuntos
Malária Falciparum , Humanos , Malária Falciparum/classificação , Malária Falciparum/patologia , Malária Falciparum/fisiopatologia , Microcirculação , Microscopia Eletrônica , Índice de Gravidade de Doença
19.
Am J Trop Med Hyg ; 69(4): 345-59, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-14640492

RESUMO

Cerebral malaria (CM) is a major cause of death in severe Plasmodium falciparum malaria. We present quantitative electron microscopic findings of the neuropathologic features in a prospective clinicopathologic study of 65 patients who died of severe malaria in Thailand and Vietnam. Sequestration of parasitized red blood cells (PRBCs) in cerebral microvessels was significantly higher in the brains of patients with CM compared with those with non-cerebral malaria (NCM) in all parts of the brain (cerebrum, cerebellum, and medulla oblongata). There was a hierarchy of sequestration with more in the cerebrum and cerebellum than the brain stem. When cerebral sequestration was compared with the peripheral parasitemia pre mortem, there were 26.6 times more PRBCs in the brain microvasculature than in the peripheral blood. The sequestration index was significantly higher in CM patients (median = 50.7) than in NCM patients (median = 6.9) (P = 0.042). The degree of sequestration of P. falciparum-infected erythrocytes in cerebral microvessels is quantitatively associated with pre-mortem coma.


Assuntos
Encéfalo/ultraestrutura , Malária Cerebral/patologia , Malária Falciparum/patologia , Plasmodium falciparum/patogenicidade , Adolescente , Adulto , Idoso , Animais , Encéfalo/irrigação sanguínea , Encéfalo/parasitologia , Cerebelo/parasitologia , Cerebelo/ultraestrutura , Eritrócitos/parasitologia , Eritrócitos/ultraestrutura , Feminino , Humanos , Masculino , Bulbo/parasitologia , Bulbo/ultraestrutura , Pessoa de Meia-Idade , Estudos Prospectivos , Telencéfalo/parasitologia , Telencéfalo/ultraestrutura , Tailândia , Vênulas/parasitologia , Vênulas/ultraestrutura , Vietnã
20.
Artigo em Inglês | MEDLINE | ID: mdl-12971509

RESUMO

We studied the pathology of acute toxoplasmosis in experimental mice inoculated with RH strain tachyzoites of Toxoplasma gondii. All died from severe disseminated toxoplasmosis involving the liver, spleen and pancreas. Pathological features of acute toxoplasmosis in susceptible mice could be regarded as an excellent model for acute reactivation of Toxoplasma in the immunosuppressed host.


Assuntos
Toxoplasma/patogenicidade , Toxoplasmose Animal/patologia , Doença Aguda , Animais , Camundongos , Microscopia Eletrônica
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