Fabrication of paper-based SERS substrate using a simple vacuum filtration system for pesticides detection.

Herein, we describe a simple and cost-effective fabrication of a paper-based SERS substrate by coating poly(diallyldimethylammonium chloride) (PDADMAC) and gold nanostars (AuNSs) on the filter paper using a vacuum filtration system. The paper-based SERS substrates were fabricated and ready to be used within an hour without any complicated equipment or processes. The cationic polymer, PDADAMAC, was pretreated on the filter paper to improve the absorbability of negatively charged AuNSs through electrostatic interaction. The PDADMAC/AuNS paper significantly intensified the SERS signal of 4-mercaptobenzoic acid (4-MBA) compared to that of pure AuNS-coated paper due to the high density of AuNSs absorbed on the SERS substrate. The PDADMAC/AuNS paper substrate provided a SERS enhancement factor (EF) of 1.08 × 107 with a low detection limit of 1 nM 4-MBA. The substrate shows excellent spot-to-spot reproducibility with a relative standard deviation (RSD) of 5.03%, and substrate-to-substrate reproducibility with an RSD of 3.20% for the Raman shift at 1080 cm-1. The paper substrate was then applied for the rapid detection of pesticides with a low detection limit of 0.51 µM (0.13 ppm) for paraquat, and 0.38 µM (0.09 ppm) for thiram, using a handheld Raman spectrometer. The development of this simple and cost-effective paper-based SERS substrate, and its applications for on-site monitoring of pesticides, could be beneficial for food security and environmental safety.

A simple fluorescence-based lateral flow test platform for rapid influenza B virus screening.

A simple fluorescence-based lateral flow test platform for rapid influenza B virus screening as a model target molecule was successfully developed. In this work, Cy5-loaded silica nanoparticles were directly conjugated to monoclonal antibodies, specific to the influenza B nucleoprotein, via a direct physisorption method and used as detector probes. Using this approach, the signal response to the detection was further determined using a fluorescent signal intensity measurement method via a portable reader, in combination with fluorescence imaging analysis. The degree to which the fluorescence signal response is detected is proportional to the amount of the target virus protein present in the system, reflected by the accumulation of the formed particle-antibody conjugates within the test system. Under optimized conditions, the system is capable of detecting the influenza B virus protein at a level of 0.55 µg per test within 30 min, using small sample volumes as low as 100 µL (R2 = 0.9544). In addition to its simplicity, further application of the system in detecting the influenza B virus protein was demonstrated using the viral transport media as specimen matrices. It was also shown that the system can perform the detection without cross-reactivity to other closely related respiratory viruses.