Coriocarcinoma cervical metastásico en mujer de 45 años con antecedente de HSIL / Metastatic choriocarcinoma in a 45-year-old woman with a previous high-grade squamous intraepithelial lesion

El coriocarcinoma es una neoplasia epitelial rara, perteneciente al grupo de las enfermedades trofoblásticas gestacionales. Se caracteriza por la presencia de células del sincitotrofoblasto, citotrofoblasto y trofoblasto intermedio, que pueden presentar invasión tisular y vascular. Resumen Mujer de 45 años, última gestación 10 años atrás. Recibe tratamiento de lesión escamosa intraepitelial de alto grado (HSIL). Tras conización, presenta sangrados irregulares. Descartada la posibilidad de enfermedad cervical residual o patología de origen endometrial, nos orientamos hacia el diagnóstico de coriocarcinoma cuando la paciente presenta hemoptisis por metástasis pulmonares. Se realiza revisión de los criterios diagnósticos, manifestaciones clínicas y pronóstico (AU)

Choriocarcinoma is a rare epithelioid neoplasm belonging to the group of gestational trophoblastic disease. The histologic feature is syncytiotrophoblastic, cytotrophoblastic and intermediate trophoblastic cells which can permeate among the myometrial fibers and vessels. AbstractWe report a case of choriocarcinoma diagnosed in a 45-year-old woman 10 years after her last pregnancy. She was treated for a high-grade squamous intraepithelial lesion (HSIL). After surgical treatment, she showed irregular vaginal bleeding. Once residual cervical disease and endometrial disease had been excluded, we suspected metastatic gestational trophoblastic disease when the patient showed hemoptysis due to pulmonary metastasis. We review the diagnostic criteria, clinical expression and prognosis of this entity (AU)

Simultaneous determination of tyramine and tryptamine and their precursor amino acids by micellar liquid chromatography and pulsed amperometric detection in wines.

Two biogenic amines, tryptamine and tyramine, and their precursors, tryptophan and tyrosine, were determined by a liquid chromatographic procedure. A hybrid micellar mobile phase of sodium dodecyl sulphate (SDS) and 1-propanol, a C18 column and electrochemical detection were used. A pH study in the range of 3-9 was performed and pH 3 was finally selected in accordance with resolution and analysis time. Oxidation potential was also checked in the range 0.6-0.9V: the maximum area obtained in all those potentials was at 0.8V, which was selected to carry out the analysis using a sequence of pulsed amperometric detection waveform. The four compounds were resolved using a mobile phase of 0.15M SDS-5% 1-propanol with an analysis time of 16 min. Repeatabilities and intermediate precision were evaluated at three different concentrations for each compound with RSD values lower than 2.6 and 4.8%, respectively. Limits of detection and quantification were also obtained within the 10-40 and 33-135 ng/ml ranges, respectively. Finally, the applicability of the procedure was tested in several types of wine and no matrix effect was observed. The possibility of direct sample introduction simplifies and greatly expedites the treatments with reduced cost, improving the accuracy of the procedures.

Determination of trazodone in urine and pharmaceuticals using micellar liquid chromatography with fluorescence detection.

A simple and reliable liquid chromatographic procedure is described for the determination of trazodone in pharmaceutical formulations and urine samples. The optimized procedure uses fluorimetric detection, a C18 column and a micellar mobile phase of sodium dodecyl sulfate (SDS) and 1-butanol. The mobile phase selected for use was 0.2M SDS and 8% 1-butanol fixed at pH 3 with phosphate buffer. The total analysis time was 10 min. For the analysis of urine samples, one great advantage of the method is that no extraction step is required. The quantification limit was 9.5 ng mL(-1), ensuring the analysis of the drug in biological fluids. The procedure shows good accuracy, repeatability and selectivity. Repeatability and intermediate precision were tested for several concentrations of the drug. Good claim percentages were obtained in the analysis of pharmaceutical formulations. Calibration repeatability in urine matrix was also studied in the 0.06-22.4 microg mL(-1) range. Good recoveries were obtained from spiked urine samples. No interferences from common additives frequently administered with trazodone or from endogenous compounds in urine samples were found. The results show that the procedure is suitable for routine analysis of the drug.

Photostability studies for micellar liquid chromatographic determination of nifedipine in serum and urine samples.

Nifedipine is a photosensitive compound that is converted into its 4-(2-nitrophenyl) pyridine and 4-(2-nitrosophenyl) pyridine homologue. In order to obtain the most adequate conditions for handling nifedipine solutions in the analytical laboratory, a number of studies on the decomposition of this compound were performed. A simple micellar liquid chromatographic procedure was described to determine nifedipine in different biological matrices such as serum and urine, and to control its decomposition. To perform the analysis, nifedipine was dissolved in 0.1 m SDS at pH 3 and chromatographed using a mobile phase containing 0.125 m SDS-3% pentanol, pH 3 on a C18 column and UV detection at 235 nm. The chromatographic analysis time was 8 min. The response of the drug for both biological matrices was linear in the 1-100 microg/mL range, with r2>0.997 at all times. Repeatability, intermediate precision (CV, %) and limits of quantification and detection (ng/mL) were 0.19, 4.3, 104 and 31 in serum and 0.81, 2.1, 136 and 41 in urine. The method developed here does not show interferences or matrix effects produced by endogenous compounds. Micellar media and mobile phases have the advantage of stabilising the compounds, thus preventing photodegradation and allowing the direct injection of biological samples.