RESUMO
The pathways by which chondrocytes of articular cartilage sense their mechanical environment are unclear. Compelling structural evidence suggests that chondrocyte primary cilia are mechanosensory organelles. This study used a 3D agarose culture model to examine the effect of compressive strain on chondrocyte cilia. Chondrocyte/agarose constructs were subjected to cyclic compression (0-15%; 1 Hz) for 0.5-48 h. Additional constructs were compressed for 48 h and allowed to recover for 72 h in uncompressed free-swelling conditions. Incidence and length of cilia labelled with anti-acetylated alpha-tubulin were examined using confocal microscopy. In free-swelling chondrocytes, these parameters increased progressively, but showed a significant decrease following 24 or 48 h compression. A 72 h recovery partially reversed this effect. The reduced cilia incidence and length were not due to increased cell division. We therefore propose that control of primary cilia length is an adaptive signalling mechanism in response to varying levels and duration of mechanical loads during joint activity.
