[Assessment of the intensity of lymphocyte blast transformation in the crypts of the palatal tonsils by using DNA cytophotometry]. / Otsenka intensivnosti blastnoi transformatsii limfotsitov v kriptakh nebnykh mindalin s pomoshch'iu tsitofotometrii DNK.

Cell fractions isolated from lacunae of palatine tonsils with different functional activities were taken from 40 children 6-15 years of age. The cell fractions were examined using vital phase-contrast microscopy, light microscopy of Pappenheim-stained smears and the Feulgen cytophotometry. Lymphocytes with signs of immunological involvement were shown to constitute 95 per cent of the whole cell lacunar population of the active functional tonsils. 35 per cent of lymphocytes, being in different stages of blast transformation, were found. The most intense blast transformation was revealed in patients with tonsil inflammation. 5-13 per cent of lacunar nuclei with DNA content above the diploid level were found in these patients. Some multicellular islets accumulating small lymphocytes, lymphocytes at different stages of blast transformation, and macrophages were revealed to imitate the cooperation of immunocompetent cells. The data obtained can be helpful in the practical surgery of palatine tonsils.

[Morphofunctional characteristics of palatine tonsil tissue and lacuna contents in chronic tonsillitis]. / Kharakteristika morfofunktsional'nogo sostoianiia tkani nebnykh mindalin i soderzhimogo lakun pri khronicheskom tonzillite.

Relationships between microbiological features and cell composition of lacunar content and morphofunctional status of the immunocompetent tissue of palatine tonsils were studied by histological, histochemical, morphometrical, microbiological, and cytological methods. Cytological and microbiological data on the lacuna content were found to reflect the reactive condition of the tonsils and should be regarded as a component of the morphofunctional characteristics of the palatine tonsils as a whole.