[Analysis of the dynamics of repeated admissions of patients to inpatient compulsory treatment in Moscow]. / Analiz dinamiki povtornykh postuplenii patsientov na prinuditel'noe lechenie v psikhiatricheskii statsionar Moskvy.

OBJECTIVE: To explore the dynamics of admission of patients for repeated compulsory treatment to a medical setting providing general and specialized psychiatric care. MATERIAL AND METHODS: The dynamics of admission of patients for repeated compulsory treatment in the «Moscow Psychiatric clinical hospital No5¼ for the period 1991-2019 was analyzed based on medical records of two groups of patients: the main group (n=1325) and the comparison group (n=15 100). RESULTS AND CONCLUSION: Over the past 5 years, there is a decrease in repeated admissions of patients to compulsory treatment who have committed a new socially dangerous act. This positive dynamic is due to compliance with the principles of continuity and phasing between inpatient and outpatient units of compulsory treatment. The transition of patients from inpatient compulsory treatment to compulsory treatment in outpatient settings is an effective measure to prevent patients from committing repeated socially dangerous acts.

["Our Patient - Our Citizen"].

The study conducted in the framework of "Our Patient - Our Citizen" Project has examined methods and possibilities of rehabilitating mentally ill people who were prone to anti-social behaviors and have been committed to involuntary treatment. The research has introduced the criteria for identifying patients with high rehabilitation potential. The work has described methods and possibilities of their rehabilitation in hospital (training psychological programs, ergotherapy, art therapy, occupational therapy, etc.) and analyzed possibility of their outpatient implementation. Concrete examples of such successful rehabilitation have been considered. Financial value was determined.

[Synthesis of prostaglandins E2 and F2alpha and quantitative changes in lipids in small intestinal mucosa in normal piglets and in piglets with diarrhea]. / Syntez prostahlandyniv E2 i F2alpha i kil'kisni zminy lipidiv u slyzovii obolontsi tonkoho kyshechnyka porosiat za normy i za diareï.

Small intestine mucose inflammation and diarrhea syndrome in piglets affected by salmonella endotoxin enhanced prostaglandin E2 synthesis and decreased prostaglandin F2 alpha synthesis. Under these conditions the share of free cholesterol decreased and the share of diacylglycerols increased in total lipid content in thin intestine mucose of piglets. The share of phosphatidic acid decreased and the share of lysophosphatidylcholine and phosphatidylinositol increased in the total content of phospholipids.

[Kinetics of 15 alpha-methyl-8-aza-16-oxagona-1,3,5(10), 13-tetraen-17-ona oxidation by cytochrome P-450 from rat liver microsomes]. / Kinetika okisleniia 15 alpha-metil-8-aza-16-oksagona-1,3,5(10), 13-tetraen-17-ona tsitokhromom P-450 mikrosom pecheni krys.

The kinetics of oxidation of 15 alpha-methyl-8-aza-16-oxagona-1,3,5(10),13-tetraen-17-on with rat liver microsomal cytochrome P-450 was investigated. The kinetic parameters, Km and Vmax, of the oxidation reaction were found to be equal to 1,3 X 10(-4) M and 4.0 X 10(-7) M X s-1, respectively. Using thin-layer chromatography, mass-spectrometry, PMR-spectroscopy and reciprocal synthesis, it was shown that 3-hydroxy-15 alpha-methyl-8-aza-16-oxagona-1,3,5(10), 13-tetraen-17-on is the main reaction product.

[Interaction of 8-aza-16-oxasteroids with rat liver microsomal cytochrome P-450]. / Vzaimodeistvie 8-aza-16-oksasteroidov s tsitokhromom P-450 mikrosom pecheni krys.

The interaction of the 8-aza-16-oxasteroid series (8-AS) with cytochrome P-450 from liver microsomes of intact and phenobarbital-induced rats has been studied. It has been shown that 8-AS are the substrates for the cytochrome P-450-dependent enzyme system, and that their affinity for cytochrome P-450 is determined by the structure of the compounds tested. Using inhibitory analysis, the site in the active center of hemoprotein responsible for 8-AS binding was examined. The possibility of direct participation of the 8-AS ketogroup in their binding with cytochrome P-450 is discussed.

[Antigenic structure of leptospires of the Canicola serogroup isolated in the north-western regions of the RSFSR]. / Kharakteristika antigennoi struktury leptospir serogruppy Canicola, vydelennykh na severo-zapade RSFSR.

The comparative study of 16 Leptospira cultures, serogroup Canicola, isolated from humans and animals in different years in the North-West of the RSFSR and 2 reference strains of the Canicola serovar, Hond Utrecht IV and Kashirsky, was carried out in the agglutinin cross-adsorption tests. The absence of the antigenic homogeneity of the cultures under test was established: 9 of them proved to be identical to strain Kashirsky and 7, to strain Hond Utrecht IV.

[Nature of the antibodies in persons inoculated with a leptospirosis vaccine]. / Priroda antitel u liudei, privitykh leptospiroznoi vaktsinoi.

The results obtained in the study of the intensity and character of immunity in persons immunized with commercial leptospirosis vaccine are presented. These results indicate that antibody formation occurs only in 12.5% of the vaccinees; antibodies are not formed to all Leptospira strains contained in the vaccine, and in 6 months all antibodies completely disappear. In persons immunized with a single vaccine dose the agglutinating activity of blood sera was linked only with IgM. In persons subjected to multiple immunizations for a number of years IgG could also be detected. Cases of leptospirosis among vaccinees have been registered.

[Comparative study of 1-naphthol oxidation by cytochrome P-450 and oxyhemoglobin]. / Sravnitel'noe izuchenie okisleniia 1-naftola s uchastiem tsitokhroma P-450 i oksigemoglobina.

The kinetics of 1-naphthol oxidation by rat liver microsomes in a phosphate buffer (pH 7.6) with participation of NADPH and O2 were studied at 9.5-27 degrees C. The activation energy of 1-naphthol oxidation (12.6 +/- 1.0 kcal/mol) was determined from the temperature dependence of initial oxidation rates. The kinetics of 1-naphthol oxidation by oxyhemoglobin in a phosphate buffer (pH 8.0) were studied at 13-25.5 degrees C. The activation energy equal to 17.7 +/- 1.0 kcal/mol for 1-naphthol oxidation by oxyhemoglobin was determined. The differences in oxidation mechanisms in the reactions with participation of cytochrome P-450 and oxyhemoglobin are discussed. Oxyhemoglobin Fe3+O2- interacts via one-electron way, whereas the microsomal cytochrome P-450 complex Fe3+O22- interacts with 1-naphthol via a two-electron way.

[Mechanisms of aliphatic alcohols oxidation by enzymatic systems of the liver]. / Mekhanizmy okisleniia alifaticheskikh spirtov fermentnymi sistemami.

The main pathways of aliphatic alcohols oxidation in human and mammalian liver, i.e. dehydration of alcohols by cytosolic alcohol dehydrogenases and oxidation in the presence of microsomal enzymatic system, catalase and hydrogen peroxide are described. A special emphasis is laid upon the interaction of alcohols with terminal oxidase of the microsomal hydroxylating system, i.e. cytochrome P-450. The relative role of these three oxidative pathways in alcohol conversions is evaluated.

[Interaction of aliphatid alcohols with cytochrome P-450 from rat liver microsomes]. / Vzaimodeistvie alifaticheskikh spirtov s tsitokhromom P-450 mikrosom pecheni krysy.

The interaction of alyphatic alcohols and cyclohexanol with cytochrome P-450 in microsomes has been investigated. All alchohols induced the modified 11 type spectral changes by mixing with microsomes. These changes are characterized by lambdamax = 412 and lambdamin = 380-382 nm in difference spectra. The dissociation constants of the alcohol cytochrome P-450 complexes are determined. On this dissociation constants influence pH and Triton X-100 presence. The interaction of the alcohols with cytochrome P-450 in phosphate buffer pH = 6,0 in the detergents absence is characterized by one dissociation constant for MeOH, EtOH, n-BuOH and cyclohexanol and by two dissociation constants for i-PrOH, i-BuOH and tert.-BuOH. The interaction of the alcohols with cytochrome P-450 in Tris-HCL-buffer (pH 7.5) in the Triton X-100 presence is characterized for all above alcohols by the dissociations constants, which are described by Taft equation with coefficient rho =-1.55. This fact confirms the interaction of alcohols HO-groups with heme iron of cytochrome P-450. The scheme of interaction of alcohols with cytochrome P-450 is discussed.

[Identification of sodium channels in vitro]. / Identifikatsiia natrievykh kanalov in vitro.

The membrane fraction isolated from cattle brain and vagal nerve was treated by ultrasonic irradiation in the presence of 22Na. It was shown that 22Na was trapped in vesicles formed from sonicated membranes in an osmotically active forms and slowly released into a nonradioactive solution. The efflux of 22Na from vesicles was studied in the presence and in the absence of substances which change the sodium conductance of excitable membranes. The local anesthetics (procaine, dibucaine) and tetrodotoxin at concentrations blocking electrical excitation in vivo decreased the sodium efflux from the vesicles. Veratrine, an activator of sodium channels, increased this efflux. The results are interpretated as an indication of the presence of functionally active sodium channels in the isolated membrane fragments.