RESUMO
The results of the molecular-epidemiological analysis of HIV-1 variants circulating in Arkhangelsk and Murmansk - northern seaports of Russia - were presented. In these seaports the HIV-1 variants belonging to subtype A1 were predominant (93% in Murmansk, 83% in Arkhangelsk). In addition to these variants, viruses of other subtypes such as B, C, D and recombinant forms CRF02_AG and CRF03_AB were identifed. The heterogeneity of circulating HIV-1 variants was higher in Arkhangelsk than in Murmansk. According to the results of phylogenetic analysis, subtype A1 sequences formed the common branch with nucleotide sequences of IDU-A strains found in other regions of Russia. HIV-1 variants of subtype B sub-clustered with sequences of East European B-variants. The recombinant strains CRF02_AG formed the common branch with HIV-1 sequences from Central Asia republics of the former USSR. Among 124 therapy-naive patients from Arkhangelsk and Murmansk (n = 124) the transmitted resistance was less than 5%.
Assuntos
Infecções por HIV/epidemiologia , HIV-1/genética , Infecções por HIV/virologia , Humanos , Filogenia , Federação RussaRESUMO
A simple and sensitive method for determining magnesium in silicates low in aluminium and iron (glass, clay, feldspars, etc.) is presented. The method is applicable in the classical procedure of silicate analysis and magnesia is determined with Eriochrome Cyanine R in an ammonium buffer solution at pH 11.5. There is no interference by elements likely to occur in such samples. The accuracy and the precision of the proposed method are superior to those of the complexometric method. The limit of detection is 0.007% MgO.
