RESUMO
OBJECTIVES: Cystatin C is a low molecular protein that has been proposed to estimate the glomerular filtration rate. Here we investigated the performance of the Roche cystatin C assay on the COBAS 6000 analyzer. DESIGN AND METHODS: We studied the imprecision, recovery, limit of detection and quantification, linearity and interferences. For method comparison, split sample aliquots were assayed using the described method and a Siemens cystatin C assay. RESULTS: The assay displayed a low total imprecision and a good linearity over the entire range tested. Bilirubin and triglycerides did not interfere with the assay, and only a haemoglobin concentration higher than 6g/dl interfered with the assay. The assay agreed well with the Siemens assay. CONCLUSION: The Roche cystatin C assay is an acceptable method for determining the cystatin C and the glomerular filtration rate estimate. On a COBAS 6000, the assay improves and simplifies the laboratory's workload.
Assuntos
Cistatina C/sangue , Taxa de Filtração Glomerular , Nefelometria e Turbidimetria/métodos , Humanos , Modelos Lineares , Nefelometria e Turbidimetria/instrumentação , Valor Preditivo dos Testes , Sensibilidade e EspecificidadeRESUMO
Nine clinical laboratories in different regions of Spain have shared the search for reference individuals and the production of reference values for urinary component-to-creatininium concentration ratios measured in first morning urine samples using RD/Hitachi