RESUMO
The induction of sister-chromatid exchanges (SCE) and chromosomal aberrations (Ch.Ab.) by the herbicide maleic hydrazide (MH) has been investigated in Chinese hamster ovary (CHO) cells grown in vitro and in bone marrow cells of mice treated in vivo. MH induces SCE and Ch.Ab. in CHO cells without metabolic activation; however, no induction of SCE was found in the in vivo experiments.
Assuntos
Cromossomos/efeitos dos fármacos , Hidrazida Maleica/toxicidade , Piridazinas/toxicidade , Animais , Medula Óssea/efeitos dos fármacos , Ciclo Celular , Linhagem Celular , Aberrações Cromossômicas , Cricetinae , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Camundongos , Troca de Cromátide Irmã/efeitos dos fármacosRESUMO
The polycyclic aromatic hydrocarbon, fluoranthene (FT), has been shown to induce SCEs in vitro in CHO cells in the presence of metabolic activation. Negative results were obtained in vivo in mice. The HPLC analysis performed to investigate the presence of metabolites of FT both in the serum of treated mice and in culture medium of CHO cells confirmed some differences between in vitro and in vivo metabolism.
