RESUMO
AIMS: To compare ELISA and Southern blot hybridisation for the detection of PCR amplified Chlamydia trachomatis DNA extracted from clinical samples; to assess the value of the ELISA method in a clinical setting. METHODS: DNA was extracted from urogenital samples of 508 patients, purified and amplified using C trachomatis specific primers, one of which was endlabelled with biotin. Amplification products were detected by Streptavidin biotin based ELISA and non-radioactive Southern blotting. RESULTS: Of the 508 samples, 29 were positive and 479 negative by both methods. No discrepant results were observed. CONCLUSION: Streptavidin biotin based ELISA and Southern blotting were equally sensitive for detecting PCR amplified C trachomatis DNA. Using ELISA, test results could be generated within a single day.
Assuntos
Infecções por Chlamydia/diagnóstico , Chlamydia trachomatis/isolamento & purificação , DNA Bacteriano/análise , Sequência de Bases , Southern Blotting , Infecções por Chlamydia/genética , Chlamydia trachomatis/genética , Ensaio de Imunoadsorção Enzimática , Humanos , Dados de Sequência Molecular , Reação em Cadeia da PolimeraseRESUMO
We present a 38-year old man suffering from a biphasic illness with fever and malaise, who developed a mild aseptic meningitis during the second phase. Lymphocytic choriomeningitis virus was found to be the causative agent.
