Advanced glycation end products, measured as skin autofluorescence, during normal pregnancy and pregnancy complicated by diabetes mellitus.

BACKGROUND: Advanced glycation end products (AGEs) accumulate with age and in diabetes mellitus (DM). AGEs can be measured by the AGE Reader (DiagnOptics Technologies BV, Groningen, The Netherlands) using skin autofluorescence (SAF). SAF is related to chronic diabetes complications. In a previous study we reported that SAF is comparable in patients with gestational DM (GDM) and controls at 27 weeks of gestation. In the current study we investigated SAF at multiple time points during pregnancy in pregnancies complicated by DM (type 1 or type 2) or GDM and in controls. Furthermore, the relation between SAF levels and adverse pregnancy outcomes was investigated. SUBJECTS AND METHODS: In this single-center prospective observational study SAF was measured during pregnancy from 26 gestational weeks onward in 79 GDM patients, 21 patients with preexistent DM (type 1 or type 2), and 55 women without diabetes. Adverse pregnancy outcomes were recorded. RESULTS: SAF decreased slightly but significantly (ß = -0.018) during normal pregnancy but not in pregnancies complicated with hyperglycemia. At the end of pregnancy SAF was higher in patients with preexistent DM (1.91 arbitrary [AU] units) compared with patients with GDM (1.71 AU) or normal pregnancy (1.66 AU) but did not differ between the latter two groups. SAF was not related to adverse pregnancy outcomes. CONCLUSIONS: The decrease in SAF during normal pregnancy could be the result of physiological changes. Because SAF was not related to adverse pregnancy outcomes, it is unlikely that the AGE Reader will be of use in daily clinical practice for GDM patients as a marker for identifying high-risk pregnancy outcomes.

Advanced glycation end products, measured as skin autofluorescence, at diagnosis in gestational diabetes mellitus compared with normal pregnancy.

BACKGROUND: Advanced glycation end products (AGEs) are tissue proteins that accumulate with age and in diabetes mellitus (DM). AGEs can be measured by the AGE-Reader (DiagnOptics Technologies BV, Groningen, The Netherlands), which measures skin autofluorescence (SAF). SAF has been suggested as a measure to screen for undiagnosed DM or impaired glucose tolerance. SAF has never been investigated in gestational DM (GDM). Therefore we compared SAF at diagnosis in GDM patients with normal pregnancy. If SAF is elevated in GDM, future research could focus on the possible use of the AGE-Reader as a screening method for GDM. METHODS: In this monocenter observational study SAF was measured in 60 GDM patients at diagnosis and 44 pregnant women without diabetes. RESULTS: SAF did not differ between GDM at diagnosis (mean [SD], 1.74 [0.31] arbitrary units) and normal pregnancy (1.76 [0.32] arbitrary units). SAF was lower in white European patients than in patients with other ethnicity. CONCLUSIONS: This first study of tissue AGE accumulation in pregnancy shows no differences in SAF between women with GDM at diagnosis and normal pregnancy. This is most likely due to mild severity and short duration of hyperglycemia in GDM at diagnosis, but it does not exclude potential differences in SAF later in pregnancy. However, the fact that no differences are detected at diagnosis makes it unlikely that the AGE-Reader can be developed as a screening method for GDM in the future. Furthermore, we found that ethnicity should be taken into account when measuring SAF.