Water activated disposable paper battery.

We developed a disposable paper battery aiming to reduce the environmental impact of single-use electronics for applications such as point of care diagnosis, smart packaging and environmental sensing. The battery uses Zinc as a biodegradable metal anode, graphite as a nontoxic cathode material and paper as a biodegradable substrate. To facilitate additive manufacturing, we developed electrodes and current collector inks that can be stencil printed on paper to create water-activated batteries of arbitrary shape and size. The battery remains inactive until water is provided and absorbed by the paper substrate, taking advantage of its natural wicking behavior. Once activated, a single cell provides an open circuit potential of 1.2 V and a peak power density of 150 µW/cm2 at 0.5 mA. As a proof of concept, we fabricated a two cell battery and used it to power an alarm clock and its liquid crystal display.

Versatile carbon-loaded shellac ink for disposable printed electronics.

Emerging technologies such as smart packaging are shifting the requirements on electronic components, notably regarding service life, which counts in days instead of years. As a result, standard materials are often not adapted due to economic, environmental or manufacturing considerations. For instance, the use of metal conductive tracks in disposable electronics is a waste of valuable resources and their accumulation in landfills is an environmental concern. In this work, we report a conductive ink made of carbon particles dispersed in a solution of shellac. This natural and water-insoluble resin works as a binder, favourably replacing petroleum-derived polymers. The carbon particles provide electrical conductivity and act as a rheology modifier, creating a printable shear-thinning gel. The ink's conductivity and sheet resistance are 1000 S m-1 and 15 Ω sq-1, respectively, and remain stable towards moisture. We show that the ink is compatible with several industry-relevant patterning methods such as screen-printing and robocasting, and demonstrate a minimum feature size of 200 µm. As a proof-of-concept, a resistor and a capacitor are printed and used as deformation and proximity sensors, respectively.

Fully 3D Printed and Disposable Paper Supercapacitors.

With the development of the internet-of-things for applications such as wearables and packaging, a new class of electronics is emerging, characterized by the sheer number of forecast units and their short service-life. Projected to reach 27 billion units in 2021, connected devices are generating an exponentially increasing amount of electronic waste (e-waste). Fueled by the growing e-waste problem, the field of sustainable electronics is attracting significant interest. Today, standard energy-storage technologies such as lithium-ion or alkaline batteries still power most of smart devices. While they provide good performance, the nonrenewable and toxic materials require dedicated collection and recycling processes. Moreover, their standardized form factor and performance specifications limit the designs of smart devices. Here, exclusively disposable materials are used to fully print nontoxic supercapacitors maintaining a high capacitance of 25.6 F g-1 active material at an operating voltage up to 1.2 V. The presented combination of digital material assembly, stable high-performance operation, and nontoxicity has the potential to open new avenues within sustainable electronics and applications such as environmental sensing, e-textiles, and healthcare.

Enzyme Activities of Five White-Rot Fungi in the Presence of Nanocellulose.

White-rot fungi can degrade all lignocellulose components due to their potent lignin and cellulose-degrading enzymes. In this study, five white-rot fungi, Trametes versicolor, Trametes pubescens, Ganoderma adspersum, Ganoderma lipsiense, and Rigidoporus vitreus were tested for endoglucanase, laccase, urease, and glucose-6-phosphate (G6P) production when grown with malt extract and nanocellulose in the form of TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical) oxidized cellulose nanofibrils (CNF) and cellulose nanocrystals (CNC). Results show that temperature plays a key role in controlling the growth of all five fungi when cultured with malt extract alone. Endoglucanase activities were highest in cultures of G. adspersum and G. lipsiense and laccase activities were highest in cultures of T. versicolor and R. vitreus. Urease activities were highest in cultures of G. adspersum, G. lipsiense, and R. vitreus. Glucose-6-phosphate levels also indicate that cells were actively metabolizing glucose present in the cultures. These results show that TEMPO-oxidized CNF and CNC do not inhibit the production of specific lignocellulose enzymes by these white-rot fungi. The apparent lack of enzymatic inhibition makes TEMPO-oxidized CNF and CNC excellent candidates for future biotechnological applications in combination with the white-rot fungi studied here.

Integrated elastomer-based device for measuring the mechanics of adherent cell monolayers.

Cells in the body collectively sustain mechanical deformations in almost all physiological functions. From the morphogenesis stage, cells' ability to sustain stress is essential for the body's well-being. Several pathologies have been associated with abnormal mechanical properties, thus suggesting the Young's modulus as a biomarker to diagnose diseases and determine their progression. Advancements in the field are quite slow because current techniques for measuring cell and tissue mechanics rely on complex and bulky measurement platforms that have low repeatability rates and limited measurement time-scales. We present the first miniaturized system that allows accurate quantification of the Young's modulus of adherent cell monolayers over a longer time (1-2 days). Our approach is based on tensile testing and optical read-out. Thanks to a thoughtful design and material choice, we are able to miniaturize tensile testing platforms into a 1 cm × 2 cm device. We provide highly repeatable Young's modulus measurements in the relevant range between 3 kPa and 300 kPa, over time and under physiological conditions, thus representing an interesting alternative to existing measurement platforms. Furthermore, the compatibility with standard biological equipment, continuous optical imaging and measurements on all types of adherent cells make this device highly versatile. Measurements on human sarcoma osteogenic (SaOS2) and Madin-Darby canine kidney cells (MDCK) are reported. The demonstrated capability to measure real-time changes in mechanical properties, such as after chemical treatment, opens the door for investigating the effects of drugs on cell mechanics.

High-speed mechano-active multielectrode array for investigating rapid stretch effects on cardiac tissue.

Systematic investigations of the effects of mechano-electric coupling (MEC) on cellular cardiac electrophysiology lack experimental systems suitable to subject tissues to in-vivo like strain patterns while simultaneously reporting changes in electrical activation. Here, we describe a self-contained motor-less device (mechano-active multielectrode-array, MaMEA) that permits the assessment of impulse conduction along bioengineered strands of cardiac tissue in response to dynamic strain cycles. The device is based on polydimethylsiloxane (PDMS) cell culture substrates patterned with dielectric actuators (DEAs) and compliant gold ion-implanted extracellular electrodes. The DEAs induce uniaxial stretch and compression in defined regions of the PDMS substrate at selectable amplitudes and with rates up to 18 s-1. Conduction along cardiomyocyte strands was found to depend linearly on static strain according to cable theory while, unexpectedly, being completely independent on strain rates. Parallel operation of multiple MaMEAs provides for systematic high-throughput investigations of MEC during spatially patterned mechanical perturbations mimicking in-vivo conditions.

An ultra-fast mechanically active cell culture substrate.

We present a mechanically active cell culture substrate that produces complex strain patterns and generates extremely high strain rates. The transparent miniaturized cell stretcher is compatible with live cell microscopy and provides a very compact and portable alternative to other systems. A cell monolayer is cultured on a dielectric elastomer actuator (DEA) made of a 30 µm thick silicone membrane sandwiched between stretchable electrodes. A potential difference of several kV's is applied across the electrodes to generate electrostatic forces and induce mechanical deformation of the silicone membrane. The DEA cell stretcher we present here applies up to 38% tensile and 12% compressive strain, while allowing real-time live cell imaging. It reaches the set strain in well under 1 ms and generates strain rates as high as 870 s-1, or 87%/ms. With the unique capability to stretch and compress cells, our ultra-fast device can reproduce the rich mechanical environment experienced by cells in normal physiological conditions, as well as in extreme conditions such as blunt force trauma. This new tool will help solving lingering questions in the field of mechanobiology, including the strain-rate dependence of axonal injury and the role of mechanics in actin stress fiber kinetics.

Assessing the degradation of compliant electrodes for soft actuators.

We present an automated system to measure the degradation of compliant electrodes used in dielectric elastomer actuators (DEAs) over millions of cycles. Electrodes for DEAs generally experience biaxial linear strains of more than 10%. The decrease in electrode conductivity induced by this repeated fast mechanical deformation impacts the bandwidth of the actuator and its strain homogeneity. Changes in the electrode mechanical properties lead to reduced actuation strain. Rather than using an external actuator to periodically deform the electrodes, our measurement method consists of measuring the properties of an electrode in an expanding circle DEA. A programmable high voltage power supply drives the actuator with a square signal up to 1 kHz, periodically actuating the DEA, and thus stretching the electrodes. The DEA strain is monitored with a universal serial bus camera, while the resistance of the ground electrode is measured with a multimeter. The system can be used for any type of electrode. We validated the test setup by characterising a carbon black/silicone composite that we commonly use as compliant electrode. Although the composite is well-suited for tens of millions of cycles of actuation below 5%, we observe important degradation for higher deformations. When activated at a 20% radial strain, the electrodes suffer from important damage after a few thousand cycles, and an inhomogeneous actuation is observed, with the strain localised in a sub-region of the actuator only.

Flexible Zinc-Tin Oxide Thin Film Transistors Operating at 1 kV for Integrated Switching of Dielectric Elastomer Actuators Arrays.

Flexible high-voltage thin-film transistors (HVTFTs) operating at more than 1 kV are integrated with compliant dielectric elastomer actuators (DEA) to create a flexible array of 16 independent actuators. To allow for high-voltage operation, the HVTFT implements a zinc-tin oxide channel, a thick dielectric stack, and an offset gate. At a source-drain bias of 1 kV, the HVTFT has a 20 µA on-current at a gate voltage bias of 30 V. Their electrical characteristics enable the switching of DEAs which require drive voltages of over 1 kV, making control of an array simpler in comparison to the use of external high-voltage switching. These HVTFTs are integrated in a flexible haptic display consisting of a 4 × 4 matrix of DEAs and HVTFTs. Using a single 1.4 kV supply, each DEA is independently switched by its associated HVTFT, requiring only a 30 V gate voltage for full DEA deflection. The 4 × 4 display operates well even when bent to a 5 mm radius of curvature. By enabling DEA switching at low voltages, flexible metal-oxide HVTFTs enable complex flexible systems with dozens to hundreds of independent DEAs for applications in haptics, Braille displays, and soft robotics.

Dielectric elastomer actuator for mechanical loading of 2D cell cultures.

We demonstrate the use of dielectric elastomer actuators (DEAs) for mechanical stimulation of cells in vitro. The development of living tissues is regulated by their mechanical environment through the modification of fundamental cellular functions such as proliferation, differentiation and gene expression. Mechanical cues have been linked to numerous pathological conditions, and progress in cellular mechanobiology could lead to better diagnosis and treatments of diseases such as atherosclerosis and cancers. Research in this field heavily relies on in vitro models due to the high complexity of the in vivo environment. Current in vitro models however build on bulky and often complex sets of mechanical motors or pneumatic systems. In this work we present an alternative approach based on DEAs, a class of soft actuators capable of large deformation (>100%) and fast response time (<1 ms). The key advantage of DEAs is that they can be integrated within the culture substrate, therefore providing a very compact solution. Here we present a DEA-based deformable bioreactor which can generate up to 35% uniaxial tensile strain, and is compatible with standard cell culture protocols. Our transparent device also includes a static control area, and enables real-time optical monitoring of both the stimulated and control cell populations. As a proof of concept we cycled a population of lymphatic endothelial cells (LECs) between 0% and 10% strain at a 0.1 Hz frequency for 24 h. We observe stretch-induced alignment and elongation of LECs, providing the first demonstration that DEAs can be interfaced with living cells and used to control their mechanical environment.