RESUMO
The herbicide bentazon (CASRN 25057-89-0) is extensively used in agriculture in Brittany (France) to replace atrazine. Bentazon is not readily adsorbed by soil and therefore it enters adjacent freshwater ecosystems, making its way to estuarine and marine waters areas. Information regarding its effects on marine ecosystems is scarce. Phytotoxicity assessments were conducted in the laboratory on the common diatom Chaetoceros gracilis using both the active ingredient and its formulated product (Basamaïs). The 3 day EC(50) using cell counts were, respectively, 150 mg/L and 60 microg/L for bentazon active ingredient and for bentazon-formulated while cellular volume was increased. Although bentazon is known as a photosystem II inhibitor, it produced an increase of pigment (chlorophylls a, c, and carotenoids) content, ATP synthesis, rates of photosynthesis and respiration, and TBARS formation. Therefore, pigments cannot be used as biomarkers of toxicity. Algal cell recovery from bentazon effects occurred after 6-9 days, suggesting a tolerance mechanism.
