Apical microleakage of radiolabeled lysozyme over time in three techniques of root canal obturation.

The three methods of obturation assessed in this study were lateral condensation (80 teeth), Thermafil (40 teeth), and McSpadden (40 teeth). All teeth were prepared to the master apical file 30 and widened coronally by Gates Glidden burs before being randomly assigned to experimental groups to be filled by each technique; they were then sealed with nail polish, except for the apical 1 mm. Quantitative evaluation of apical microleakage for each technique was obtained after periods of 1 day, 7 days, 14 days, and 28 days of immersion in a lysozyme solution labeled with radioactive iodine by preparing horizontal sections of the teeth and measuring the level of radioactivity in each section using a gamma counter. Initial (1-day) leakage was least in the Thermafil group and was significantly different from the other techniques. Leakage was greatest in the laterally condensed samples. By the end of the study (28th day) values for lateral condensation were lowest, but were significantly different only for the McSpadden group. For all techniques leakage was most significant in the first 3 mm from the apex and was very low below this level so that all methods can be considered as giving a hermetic seal below 3 mm.

Dissolution of synthetic hydroxyapatite in the presence of acidic polypeptides.

This article deals with the effect of two acidic polypeptides [polyaspartic acid (PA) and polyglutamic acid (PG)] onto hydroxyapatite (HAP) dissolution by separately considering their influence when they are present only at the HAP interface and when they are both adsorbed and present in the bulk solution. We first determined the amount of adsorbed PA and PG at pH 7.0 and 5.0 onto 10 mg of HAP. Dissolution experiments were performed at pH 5.0 under pH stat conditions by continuously following the consumed protons and released calcium versus time with the aid of specific electrodes. The released phosphate ions were determined by spectrophotometric analysis. The data show that, because of their calcium chelating properties, the polypeptides act as a driving force for HAP dissolution when PA and PG remain present in solution and the interfacial beneficial effect of the adsorbed peptides is erased by the chelating properties of PA and PG present in the solution. When the polypeptides are only adsorbed at the interface, even if a partial PA or PG desorption occurs, HAP dissolution inhibition is still observed.