Fortification/enrichment of milk and dairy products by encapsulated bioactive ingredients.

Bioactive compounds (bioactives) derived from plants and animals, are effective in increasing the safety and health of society through the treatment and prevention of diseases such as cancer. Fortifying conventional foods with bioactives is an accepted strategy by scientists, food manufacturers, and consumers. Milk and dairy products are among the most important foods used in our daily diet and can be a suitable option to deliver bioactives to the body, but there are challenges towards using these compounds in their original unprotected/free form. They can be degraded before reaching the target location in the body and interact with milk compounds, resulting in a negative impact on the quality characteristics of the corresponding foods. Thus, a suitable encapsulation technique can help to protect these sensitive compounds from environmental stresses and the process they encounter during the manufacture of food. This also prevents adverse interactions of bioactives with compounds in milk. This article aimed to review the recent literature about the addition of encapsulated bioactives such as vitamins, essential fatty acids, phenolic compounds, minerals, and enzymes into milk and dairy products, with a focus on common applied bioactives, methods of encapsulation, the interaction of bioactives with milk components, and the challenges facing the use of this technology in the dairy industry.

Application of Cornelian Cherry (Cornus mas L.) Peel in Probiotic Ice Cream: Functionality and Viability during Storage.

In this study, cornelian cherry (Cornus mas L.) peel (CCP) was incorporated into a probiotic ice cream formulation containing Bifidobacterium lactis to investigate the potential effect of CCP on the viability of B. lactis in the ice cream after simulated gastrointestinal stress and during 120 days of storage. Furthermore, the effect of the addition of CCP (3, 6, and 9%) on bioactive compounds, antioxidant activity, and physicochemical and sensory attributes of the ice cream was evaluated. The results showed that the addition of CCP significantly enhanced vitamin C, total polyphenols, total anthocyanin content, and antioxidant activity of the ice cream. During frozen storage of the ice cream, phenolic compounds and anthocyanins were quite stable, but vitamin C significantly decreased. The addition of CCP had no significant effect on the viability of B. lactis throughout the freezing process, but increments of 6% and 9% CCP increased the viability of B. lactis in the ice cream and after simulated gastrointestinal processes in all storage periods. These findings imply that CCP is a promising candidate to be used for producing functional ice cream.

Optimization of extraction and deamidation of edible protein from evening primrose (Oenothera biennis L.) oil processing by-products and its effect on structural and techno-functional properties.

The optimization of ultrasound-assisted alkaline extraction and enzymatic deamidation by protein-glutaminase (PG) on evening primrose seed cake (EPSC) protein and its effect on structure (amino acid composition, secondary structure and electrophoresis pattern) and techno-functional properties (water-holding and oil-binding capacities, solubility, emulsifying and foaming properties) of EPSC protein were evaluated. The optimum conditions of the both processes were measured using response surface methodology (RSM). The maximum yield (26.4%) and protein content (86.1%) were reached at the optimized extraction conditions. Optimal conditions of PG deamidation based on reaching a high degree of deamidation (DD) with a simultaneously low degree of hydrolysis (DH). Under these conditions, DD and DH were 39.40 and 2.11%, respectively. Ultrasound-assisted alkaline extraction and enzymatic deamidation by PG have great potential to produce edible EPSC protein with modified techno-functional characteristics that can be used for several aims in the food and pharmaceutical applications.

Chitosan nanoparticles loaded with clove essential oil: Characterization, antioxidant and antibacterial activities.

One of the recent trends in the food industry is application of natural antioxidant/antimicrobial agents. In this study, essential oil of clove buds was extracted and encapsulated in chitosan nanoparticles using a two-step technique of emulsion-ionic gelation. A good retention rate (55.8-73.4 %) of clove essential oil (CEO) loaded in chitosan nanoparticles was confirmed. Also, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses revealed the success of CEO encapsulation. Scanning electron microscopy (SEM) images illustrated regular distribution and spherical shape of nanoparticles with a size range of 223-444 nm. The antioxidant activity of CEO-loaded chitosan nanoparticles was higher than free CEO. Similarly, CEO-loaded chitosan nanoparticles had a high antibacterial activity against L. monocytogenes and S. aureus (inhibition halo diameter of 4.80-4.78 cm). This technique could improve the efficiency of CEO in food products and a delivery system for novel applications such as active packaging.