Non-linear models of 15N partitioning kinetics in late lactation dairy cows from individually-labeled feed ingredients.

Few studies have examined the N kinetics of individual feeds with stable isotope tracing. We hypothesized that N partitioning to milk and excreta pools as well as the rates of the processes that drive this partitioning would differ for alfalfa silage, corn silage, corn grain, and soybean meal. Feed ingredients were endogenously labeled with 15N and included in 4 diets to create treatments with the same dietary composition and different labeled feed. Diets were fed to 12 late-lactation dairy cows for 4 d (96 h) and feces, urine, and milk collection proceeded during the 4 d of 15N enrichment and for 3 d (80 h) after cessation of label feeding. Nonlinear models of 15N enrichment and decay were fit to milk (MN), urine (UN), and fecal N (FN) in R with the nlme package and feed-specific parameter estimates were compared. The estimated proportions of feed N that were excreted in feces supported our understanding that N from soybean meal and corn grain is more digestible than N from alfalfa and corn silage. Estimates for the N partitioning between milk (MN) and urine (UN) from the 2 concentrate feeds (soybean meal and corn grain) indicated that UN:MN ratios were less than or equal to 1:1 indicating either more or equal nitrogen partitioning to milk compared with urine. It is important to maintain factual accuracy in representing the results rather than implying a desired outcome unsupported by the data. In contrast, UN:MN ratios for forage feeds (corn and alfalfa silage) were > 1:1, indicating more N partitioning to urine than milk. The modeled proportion of total FN that originated from feed N was 82.2% which is in line with previous research using a similar 15N measurement timeframe. However, the proportion of urinary and MN originating from feed N was much lower (60.5% for urine, 57.9% for milk), suggesting that approximately 40% of urinary and MN directly originate from body N sources related to protein turnover.

Soil greenhouse gas flux and nitrogen mineralization following manure application from tannin-fed dairy cows.

Growing concerns about environmental impacts of dairy farms have driven producers to address greenhouse gas (GHG) emissions and nitrogen (N) losses from soil following land application of dairy manure. Tannin dietary additives have proved to be a successful intervention for mitigating GHG and ammonia (NH3 ) emissions at the barn scale. However, it is unknown how land application of dairy manure from cows fed tannin diets affects crop-soil nitrogen dynamics and soil GHG flux. To test this, cows were fed diets at three levels of tannins (0.0%, 0.4%, and 1.8% of dry matter intake) and their manure was field applied at two N rates (240 and 360 kg N ha-1 ). Soil NH4 + -N, NO3 - -N, corn silage yield, and soil GHG flux were then measured over a full growing season. Soils amended with tannin manure had lower initial NH4 + -N concentrations and lower total mineral N (NH4 + -N + NO3 - -N) concentrations 19 days after application, compared to soils amended with no tannin manures. Despite lower early season N availability in tannin-fertilized plots, there were no differences in corn silage yield. No differences in soil GHG and NH3 emissions were observed between manure-amended treatments. These results demonstrate that while tannin addition to dairy cow feed does not offer short-term GHG or NH3 emissions reductions after field manure application, it can promote slower soil N mineralization that may reduce reactive N loss after initial application.

Estimating Excreted Nutrients to Improve Nutrient Management for Grazing System Dairy Farms.

Improving nutrient management in grazing system dairy farms requires determining nutrient flows through animals, the placement of cows within farms and potential for collection, and the re-use and loss of nutrients. We applied a model incorporating data collected at a range of temporal and spatial scales to quantify nutrient excretion in all locations that lactating herds visited on five days over a year on 43 conventional and organic grazing system dairy farms. The calculated nutrient loads excreted by cows in different places were highly skewed; while N, P and K deposited loads were consistent across the year, S, Ca and Mg loads varied between sampling times and seasons. The greatest mean and range in nutrient loads were deposited in paddocks, with the smallest amounts deposited in dairy sheds. All excreted nutrient loads increased with farm and herd sizes and milk production. Mean daily loads of 112, 15, 85, 11, 22 and 13 kg of N, P, K, S, Ca and Mg were deposited by the herds which, when standardised to a 305-day lactation, amounted to 24, 4, 20, 3, 5 and 3 t excreted annually, respectively. In addition to routine manure collection in dairy sheds, ensuring collection and recycling of nutrients excreted on feed pads and holding areas would decrease potential nutrient losses by 29% on average. Non-collected, recycled nutrients were disproportionately returned to paddocks in which cows spent time overnight, and except for S and Ca, nutrient loading rates were greater than rates applied as fertilisers. These data demonstrate the extent of excreted nutrients in grazing dairy systems and indicate the need to account for these nutrients in nutrient management plans for Australian dairy farms. We propose incorporating excretion data in current budgeting tools using data currently collected on most Australian grazing system dairy farms.

Nutrient Intake, Excretion and Use Efficiency of Grazing Lactating Herds on Commercial Dairy Farms.

Estimating excreted nutrients is important for farm nutrient management, but seldom occurs on commercial grazing system farms due to difficulties in quantifying pasture intake. Nitrogen (N), phosphorus (P), potassium (K), sulphur (S), calcium (Ca) and magnesium (Mg) intake, excretion and use efficiency of 43 commercial dairy herds grazing pasture were calculated to understand the range in nutrient intake and excretion in these systems. Milk production, feed (grazed and supplement), as well as farm and herd management data were collected quarterly on representative farms located in temperate, arid, subtropical and tropical regions of Australia. Lactating herd sizes on these farms averaged 267 (30 to 1350) cows, with an average daily milk yield of 22 (9 to 36) kg/cow per day and the herds walked from <0.01 to 4 km/day on a variety of terrains. The mean total metabolizable energy (ME) required by cows in the herds was estimated to be 195 (116 to 289) MJ/cow per day. Although these farms are considered grazing systems, feeding strategies ranged from total dependence on pasture to total mixed rations (TMRTMR) and consisted of a wide variety of nutrient and energy contents. Mean pasture dry matter intake (DMI) (9 kg/cow per day, from 0.1 to 22 kg/cow per day) was just over half of total DMI. Dietary concentration of crude protein, P, K, S, Ca and Mg concentrations were, on average, 19%, 0.45%, 2.1%, 0.29%, 0.65%, and 0.3%, respectively, for all herds and, except for N, supplement nutrient concentrations were always more variable than pasture. Approximately 72% and 88% of diets provided greater than recommended P and N intakes, respectively. Calculated mean N, P, K, S, Ca and Mg excretions were 433, 61, 341, 44, 92 and 52 g/cow per day, respectively. Of the farm characteristics examined, residual maximum likelihood (REML) analysis indicated that daily excreted N, P and S were significantly related to per ha milk production, and excreted P, K and Mg were related to percentage of herd DMI provided as supplement. Mean use efficiencies by cows of N, P, K, S, Ca and Mg were 21%, 25%, 9%, 16%, 23% and 4%, respectively. These estimates of nutrient excretion and feed nutrient use efficiencies can be used to improve nutrient management on grazing system commercial dairy farms.

Links among nitrification, nitrifier communities, and edaphic properties in contrasting soils receiving dairy slurry.

Soil biotic and abiotic factors strongly influence nitrogen (N) availability and increases in nitrification rates associated with the application of manure. In this study, we examine the effects of edaphic properties and a dairy (Bos taurus) slurry amendment on N availability, nitrification rates and nitrifier communities. Soils of variable texture and clay mineralogy were collected from six USDA-ARS research sites and incubated for 28 d with and without dairy slurry applied at a rate of ~300 kg N ha(-1). Periodically, subsamples were removed for analyses of 2 M KCl extractable N and nitrification potential, as well as gene copy numbers of ammonia-oxidizing bacteria (AOB) and archaea (AOA). Spearman coefficients for nitrification potentials and AOB copy number were positively correlated with total soil C, total soil N, cation exchange capacity, and clay mineralogy in treatments with and without slurry application. Our data show that the quantity and type of clay minerals present in a soil affect nitrifier populations, nitrification rates, and the release of inorganic N. Nitrogen mineralization, nitrification potentials, and edaphic properties were positively correlated with AOB gene copy numbers. On average, AOA gene copy numbers were an order of magnitude lower than those of AOB across the six soils and did not increase with slurry application. Our research suggests that the two nitrifier communities overlap but have different optimum environmental conditions for growth and activity that are partly determined by the interaction of manure-derived ammonium with soil properties.

Estimates of residual dairy manure nitrogen availability using various techniques.

It is common practice to repeatedly apply dairy manure to the same fields. To accurately assess the total plant availability of manure nutrients, it is necessary to account for the nutrients remaining in soil from previous manure applications. A field experiment studying manure nitrogen (N) uptake by corn (Zea mays L.) was conducted from 1998 to 2003 on a Plano silt loam (fine-silty, mixed, mesic, Typic Argiudolls). Plots received two rates of semisolid manure either every year, every 2 yr, or every 3 yr to estimate first-, second-, and third-year dairy manure N residuals. Residual manure N availability was estimated from single and multiple manure applications using (i) the fertilizer N equivalence method, (ii) the apparent recovery (difference) method, (iii) relative effectiveness method, and (iv) recovery of (15)N-labeled manure. Second-year availabilities after a single manure application using the fertilizer equivalence, difference, and relative effectiveness methods were estimated to be 12, 8, and 4% of total manure N applications, respectively. Estimates of third-year availability by these methods were 3, 1, and 5%, respectively. Measurement of (15)N recovered from labeled manure was 6 and 2% in the second and third year, respectively. Fertilizer equivalence, difference, and relative effectiveness methods showed great year to year variability, reducing the confidence in the residual manure N availability estimates by these methods, but using (15)N-labeled manures reduced variability substantially. Based on this and other studies, we suggest that second- and third-year residual N availability from a single application of semisolid dairy manure would be 9 to 12%, and 3 to 5% of the original manure N application, respectively.

Manure collection and distribution on Wisconsin dairy farms.

Manure management plans require knowing the amount of manure produced, collected, and available for land-spreading. Whereas much information is available to calculate manure production, little is known about the types and amounts of manure actually collected on typical dairy farms. This study of 54 representative Wisconsin dairy farms showed significant regional, housing, and herd size differences in collection of manure from lactating cows (Bos taurus), dry cows, and heifers. Significantly (P < 0.05) less manure is collected in the hilly southwest (56% of total annual herd production) than in the undulating south central (72%) or the flat northeast (68%) regions. Collection of lactating cow manure is significantly (P < 0.05) lower from stanchion (66% of total annual production) than free-stall (89%) housing, and significant (P < 0.05) positive relationships were found between the number of lactating cows a farm keeps and the percentage manure collected. Average annual manure N (range of 116-846 kg N ha(-1)) and P (range of 24-158 kg P ha(-1)) loading rates in areas where manure goes uncollected was highest in unvegetated barnyards followed by vegetated and partially vegetated outside areas. Once uncollected manure was accounted for, average annual loading rates on cereal cropland ranged from 128 to 337 kg ha(-1) of manure N, and from 45 to 139 kg ha(-1) of manure P. Compared with adjacent cropland, the accumulation of uncollected manure has vastly increased soil test P, K, and organic matter levels in outside areas. Manure management on Wisconsin dairy farms with small to medium herds might require assistance in managing manure in outside confinement areas to reduce the risk of impairing surface and ground water quality.

Corn residue level and manure application timing effects on phosphorus losses in runoff.

Growing interest in corn (Zea mays L.) silage utilization on Wisconsin dairy farms may have implications for nutrient losses from agricultural lands. Increasing the silage cutting height will increase residue cover and could reduce off-site migration of sediments and associated constituents compared with conventional silage harvesting. We examined the effects of residue level and manure application timing on phosphorus (P) losses in runoff from no-till corn. Treatments included conventional corn grain (G) and silage (SL; 10- to 15-cm cutting height) and nonconventional, high-cut (60-65 cm) silage (SH) subjected to different manure application regimes: no manure (N) or surface application in fall (F) or spring (S). Simulated rainfall (76 mm h(-1); 1 h) was applied in spring and fall for two years (2002-2003), runoff from 2.0- x 1.5-m plots was collected, and subsamples were analyzed for dissolved reactive phosphorus (DRP), total phosphorus (TP), and P mass distribution in four particle size classes. Total P and DRP loads were inversely related to percent residue cover, but both TP and DRP concentrations were unaffected by residue level. Manure application increased DRP concentrations in spring runoff by two to five times but did not significantly affect DRP loads, since higher concentrations were offset by lower runoff volumes. Spring manure application reduced TP loads in spring runoff by 77 to 90% compared with plots receiving no manure, with the extent of reductions being greatest at the lower residue levels (<24%). The TP concentration in sediments increased as particle size decreased. Manure application increased the TP concentration of the 0- to 2-microm fraction by 79 to 125%, but elevated the 2- to 10- and 10- to 50-microm fractions to a lesser extent. Recent manure additions were most influential in enriching transported sediments with P. By itself, higher residue cover achieved by high-cutting silage was often insufficient to lower P losses; however, the combination of manure application and higher residue levels significantly reduced P losses from corn fields harvested for silage.

Residue level and manure application timing effects on runoff and sediment losses.

There is growing interest in evaluating the effects of corn silage harvesting methods on erosion control. Increasing the silage cutting height will increase residue cover and could conceivably minimize off-site migration of sediments compared with conventional silage harvesting. The effects of residue level and manure application timing on runoff and sediment losses from no-till corn were examined. Treatments included conventional corn grain (G) and silage (SL) and nonconventional, high-cut (60-65 cm) silage (SH). Corn harvesting treatments were subjected to different manure application regimes: no manure (N) or surface application in fall (F) or spring (S). Simulated rainfall (76 mm/h; 1 h) was applied in spring and fall for two years (2002-2003), runoff from 2.0- x 1.5-m plots collected, and a subsample analyzed for sediment concentration and aggregate size distribution. Runoff volume was inversely related to residue cover. Manure addition to silage plots reduced spring runoff by 71 to 88%, attributable to an increase in soil organic matter content, compared with SH-N and SL-N. Differences in sediment concentration between SH and SL were not significant. For silage plots, spring-applied manure had the greatest influence on sediment export reducing it by 84 to 93% in spring runoff compared with corresponding N plots. Sediment loads were also 85 to 97% lower from SH-S compared with SL-N in all four seasons. Except for spring 2003, sediment export was lower from G compared with SL. The combination of manure and higher residue associated with high-cut silage often lowered sediment export compared with low-cut silage. Nearly identical aggregate size distributions were observed in sediments from SH and SL plots. High residue levels combined with spring-applied manure led to enrichment in the clay-sized fraction of runoff sediment. Recently applied manure and higher residue levels achieved by high-cutting silage can substantially lower sediment losses in spring runoff when soil is most susceptible to erosion.