RESUMO
PURPOSE: Myofascial release techniques at the time of complex hernia repair allow for tension-free closure of the midline fascia. Two common techniques are the open external oblique release (EOR) and the transversus abdominis release (TAR). Each technique has its reported advantages and disadvantages, but there have been few comparative studies. The purpose of this project was to compare the outcomes of these two myofascial release techniques. METHODS: The Americas Hernia Society Quality Collaborative (AHSQC) database was queried and produced a data set on 24 May 2018. All patients undergoing open incision hernia repair with an open EOR or TAR were evaluated, and outcomes were compared including hernia recurrence, quality of life, and 30-day wound-related complications. RESULTS: 3610 patients met the inclusion criteria of undergoing open incisional hernia repair (501 undergoing EOR and 3109 undergoing TAR). Seventy surgeons from 50 institutions contributed EOR patients, and 124 surgeons from 89 institutions contributed TAR patients with no differences between the two groups in surgeons' affiliation. Comparing open EOR and TAR showed no significant differences in hernia recurrence, quality of life, or 30-day surgical site infection rate. EOR had a significantly higher rate of surgical site occurrences compared with TAR (p < 0.05); however, this did not result in an increase in surgical site occurrences requiring procedural interventions. CONCLUSIONS: Equivalent outcomes were achieved using the EOR or TAR techniques in the open repair of incisional hernias. Both techniques offer consistently good outcomes and are important adjuncts in the repair of complex incisional hernias.
Assuntos
Hérnia Ventral , Hérnia Incisional , Músculos Abdominais/cirurgia , Hérnia Ventral/cirurgia , Herniorrafia/efeitos adversos , Humanos , Hérnia Incisional/cirurgia , Qualidade de Vida , Telas CirúrgicasRESUMO
Reactive nitrogen (Nr) species include oxides of nitrogen [N; nitric oxide, nitrogen dioxide and nitrous oxide (NO)], anions (nitrate and nitrite), and amine derivatives [ammonia (NH), ammonium salts and urea]. Of the different Nr species, air emissions from swine and poultry facilities are predominantly NH followed by NO. Excreta emissions are NH, ammonium ions, and urea with trace amounts of nitrate and nitrite. Farm systems and practices that handle manure as a wet product without pH modification favor almost exclusive NH production. Systems and practices associated with dry manure handling and bedded systems emit more NH than NO. Results from a turkey grow-out study estimated that just under 1% of consumed N was emitted as NO from housing, compared with just under 11% emitted as NH. Despite generally less NO emissions from animal housing compared with crop field emissions, NO emissions from housing are often greater than estimated. Lagoon systems emit more NO than either slurry or deep pit swine systems. Deep pit swine buildings emit only one-third the NO that is emitted from deep bedded swine systems. Laying hen, broiler chicken, and turkey buildings emit over 4 times as much NO as swine housing, on a weight-adjusted basis. Critical control points for mitigation center on: 1) reducing the amount of N excreted and, therefore, excreted N available for loss to air or water during housing, manure storage, or following land application of manures; 2) capturing excreted N to prevent release of N-containing compounds to air, water, or soil resources; or 3) conversion or treatment of N-containing compounds to non-reactive N gas.
Assuntos
Amônia/metabolismo , Nitrogênio/metabolismo , Óxido Nitroso/metabolismo , Aves Domésticas/metabolismo , Espécies Reativas de Nitrogênio/metabolismo , Suínos/metabolismo , Amônia/análise , Criação de Animais Domésticos , Animais , Poluição Ambiental , Recuperação e Remediação Ambiental , Abrigo para Animais , Esterco/análise , Nitrogênio/análise , Óxido Nitroso/análise , Espécies Reativas de Nitrogênio/análise , Ureia/análise , Ureia/metabolismoRESUMO
Managing the demands of an academic appointment in extension can be a challenging task. Demands from constituent groups, expectations of supervisors, and rigors of promotion and tenure processes can create pressures that young faculty did not expect. Throw in spousal and family duties and you have created a situation that many will find hard to navigate. However, there are ways to cope and, even better news, there are ways to excel in meeting the demands of an academic appointment and enjoying life. Because many new extension faculty members do not have prior experience in extension, best practices in documenting programs and extension scholarship over the pretenure period are provided in this paper. Appointments that include both research and extension are quite common at many land grant universities. The advantages of joint appointments are numerous and include the fact that more and more grant agencies are seeking integrated research, teaching, and/or extension projects. However, the time demands of joint appointments can be challenging. Joint appointments can be designed to help faculty members conduct important translational research and have it be applied in a production setting. By seeking commonalities in research and extension efforts, joint appointments can be very synergistic. Development of highly successful programs requires planning on the front end with an emphasis on an in-depth needs assessment to determine stakeholder needs for both research and extension. Impact assessment should be part of this planning effort. Performing as a successful extension faculty member while maintaining relationships outside of work is challenging and requires deliberate effort on the part of employees and supervisors to realize there is more to life than work. Some authors have referred to this as work-life balance, but it may be more helpful to think of it as work-life effectiveness. To do this, one needs to 1) define what success looks like, 2) set boundaries and maintain control including control of your schedule, and 3) find time to ensure your physical, emotional, and spiritual well-being are nurtured in addition to your professional development. In summary, extension careers can be challenging at times as demands and expectations of stakeholders, supervisors, and rigors of the tenure system create formidable obstacles. However, by keeping a focus on the priorities of the position and looking for synergy in research and extension work, they can actually be quite enjoyable and very rewarding.
Assuntos
Educação em Veterinária/organização & administração , Docentes , Universidades , Medicina Veterinária , Equilíbrio Trabalho-Vida , Animais , Humanos , Médicos VeterináriosRESUMO
Two experiments evaluated the effect of calcium ammonium nitrate decahydrate (calcium nitrate [NIT]) and monensin sodium (MON) on in vitro fermentation parameters of 2 contrasting diets (100:0 and 10:90 forage-to-concentrate ratios). Diet addition of NIT (0, 1.25, and 2.5 g/100 g DM) and MON (0, 3, and 6 mg/L) were tested alone and combined (9 treatments total; 5 bottles per treatment). Mixed ruminal microorganisms were incubated in anaerobic media containing 0.5 g of substrate diet, 1 of 9 treatments, and 40 mL buffer solution. Incubations were performed in batch cultures for 48 h at 39°C. Headspace gas volume was measured and sampled at 4, 8, 12, 24, and 48 h, and the VFA profile was assessed at the end of the experiment. Total gas production was reduced by NIT (87.9 vs. 94.6 mL; < 0.01) and MON (78.6 vs. 94.6 mL; < 0.01) and, in Exp. 2, further reduced by NIT+MON when the additives were combined (161.1 vs. 196.9 mL; < 0.01). Methane production from control in Exp. 1 and Exp. 2 averaged 9.1 and 15.3 mL, respectively, and was decreased by NIT (3.4 and 8.3 mL in Exp. 1 and Exp. 2, respectively; P < 0.01), MON (4.1 and 7.7 mL; in Exp. 1 and Exp. 2, respectively; < 0.01) and NIT+MON (1.1 and 1.5 mL; in Exp. 1 and Exp. 2, respectively; < 0.01). Both experiments demonstrated a significant increase in nitrous oxide (NO; < 0.01) when NIT was added. Compared to the control treatment, IVDMD was reduced when NIT+MON was added at the higher doses in EXP1 (31.7 vs. 37.4%; < 0.01) and EXP2 (76.6 vs. 79.9 %; < 0.01). Net VFA production was not affected by treatments ( > 0.10), but molar proportions of acetate and butyrate were reduced by MON ( < 0.01). Propionate molar proportion was increased in both experiments by MON ( < 0.01) and further increased in Exp. 2 when the additives were combined at lower doses ( < 0.01). Compared to the control treatment, the acetate:propionate (A:P) ratio was reduced by MON in Exp. 1(1.2 vs. 2.8; < 0.01) and Exp. 2 (1.0 vs. 2.3; < 0.01). Fermentation efficiency (%) was increased by MON (81.7 vs. 73.7%; < 0.01) and further increased in Exp. 2 when the additives were combined at lower doses (87.2 vs. 76.6%; < 0.01). The combination of NIT and MON in 2 contrasting diets proved beneficial by altering fermentation products toward lower CH and more propionate; however, the addition of NIT consistently increased NO production. Negative effects of the additives on IVDMD were found only when the additives were combined at higher doses.
Assuntos
Compostos de Cálcio/farmacologia , Bovinos/metabolismo , Fermentação/efeitos dos fármacos , Metano/metabolismo , Monensin/farmacologia , Nitratos/farmacologia , Rúmen/efeitos dos fármacos , Ração Animal/análise , Animais , Dieta/veterinária , Digestão/efeitos dos fármacos , Feminino , Rúmen/metabolismoRESUMO
Given increased interest by all stakeholders to better understand the contribution of animal agriculture to climate change, it is important that appropriate methodologies be used when measuring greenhouse gas (GHG) emissions from animal agriculture. Similarly, a fundamental understanding of the differences between methods is necessary to appropriately compare data collected using different approaches and design meaningful experiments. Sources of carbon dioxide, methane, and nitrous oxide emissions in animal production systems includes the animals, feed storage areas, manure deposition and storage areas, and feed and forage production fields. These 3 gases make up the primary GHG emissions from animal feeding operations. Each of the different GHG may be more or less prominent from each emitting source. Similarly, the species dictates the importance of methane emissions from the animals themselves. Measures of GHG flux from animals are often made using respiration chambers, head boxes, tracer gas techniques, or in vitro gas production techniques. In some cases, a combination of techniques are used (i.e., head boxes in combination with tracer gas). The prominent methods for measuring GHG emissions from housing include the use of tracer gas techniques or direct or indirect ventilation measures coupled with concentration measures of gases of interest. Methods for collecting and measuring GHG emissions from manure storage and/or production lots include the use of downwind measures, often using photoacoustic or open path Fourier transform infrared spectroscopy, combined with modeling techniques or the use of static chambers or flux hood methods. Similar methods can be deployed for determining GHG emissions from fields. Each method identified has its own benefits and challenges to use for the stated application. Considerations for use include intended goal, equipment investment and maintenance, frequency and duration of sampling needed to achieve desired representativeness of emissions over time, accuracy and precision of the method, and environmental influences on the method. In the absence of a perfect method for all situations, full knowledge of the advantages and disadvantages of each method is extremely important during the development of the experimental design and interpretation of results. The selection of the suitable technique depends on the animal production system, resource availability, and objective for measurements.
Assuntos
Ração Animal/análise , Criação de Animais Domésticos/métodos , Dióxido de Carbono/química , Gases , Esterco/análise , Metano/química , Poluentes Atmosféricos , Animais , Mudança Climática , Efeito Estufa , Metano/metabolismo , Modelos Teóricos , Óxido NitrosoRESUMO
Greenhouse gas (GHG) fluxes and soil organic carbon (SOC) accumulation in grassland ecosystems are intimately linked to grazing management. This study assessed the carbon equivalent flux (Ceq) from 1) an irrigated, heavily stocked, low-density grazing system, 2) a nonirrigated, lightly stocked, high-density grazing system, and 3) a grazing-exclusion pasture site on the basis of the GHG emissions from pasture soils and enteric methane emissions from cows grazing different pasture treatments. Soil organic carbon and total soil nitrogen stocks were measured but not included in Ceq determination because of study duration and time needed to observe a change in soil composition. Light- and heavy-stocking systems had 36% and 43% greater Ceq than nongrazed pasture sites, respectively ( < 0.01). The largest contributor to increased Ceq from grazing systems was enteric CH emissions, which represented 15% and 32% of the overall emissions for lightly and heavily stocked grazing systems, respectively. Across years, grazing systems also had increased nitrous oxide (N2O; < 0.01) and CH emissions from pasture soils ( < 0.01) compared with nongrazed pasture sites but, overall, minimally contributed to total emissions. Results indicate no clear difference in Ceqflux between the grazing systems studied when SOC change is not incorporated ( = 0.11). A greater stocking rate potentially increased total SOC stock ( = 0.02), the addition of SOC deeper into the soil horizon ( = 0.01), and soil OM content to 30 cm ( < 0.01). The incorporation of long-term annual carbon sequestration into the determination of Ceq could change results and possibly differentiate the grazing systems studied.
Assuntos
Criação de Animais Domésticos , Ciclo do Carbono , Bovinos/fisiologia , Ecossistema , Poluentes Atmosféricos , Animais , Feminino , Metano/metabolismo , Nitrogênio/metabolismo , Óxido Nitroso/metabolismo , SoloRESUMO
The objective of this study was to test the hypothesis that reducing dietary CP by 1.5% and supplementing crystalline AA (CAA) to meet the standardized ileal digestible (SID) AA requirements for growing and finishing pigs decreases air emissions of ammonia (NH), nitrous oxide (NO), and carbon dioxide (CO) compared with an industry standard diet, without reducing growth performance. Seventy-two pigs were allocated to 12 rooms (6 pigs per room) and 2 diets (6 rooms per diet) formulated according to a 5-phase feeding program across the grow-finish period (107 d total). The diets consisted of a standard diet containing 18.5 to 12.2% CP or a reduced CP diet containing 17.5 to 11.0% CP + CAA over the course of the 5-phase feeding program. Gases (NH, NO, hydrogen sulfide, methane, nonmethane total hydrocarbon, and CO) and ventilation rates were measured continuously from the rooms. Compared with standard diet, ADG and feed conversion of pigs fed reduced CP + CAA diets did not differ (2.7 kg gain/d and 0.37 kg gain/kg feed, respectively). Compared with standard diet, feeding reduced CP + CAA diets decreased ( < 0.01) NH emissions by 46% over the 107-d period (5.4 and 2.9 g · pig · d, respectively). Change in NH emissions for each percentage unit reduction in dietary CP concentration corresponded with 47.9, 53.2, 26.8, 26.5, and 51.6% during Phases 1 through 5, respectively. Emissions of other gases did not differ between diets. Feeding reduced CP diets formulated based on SID AA requirements for grow-finisher swine is effective in reducing NH emissions from housing compared with recent industry formulations and does not impact growth performances.
Assuntos
Aminoácidos/metabolismo , Ração Animal/análise , Proteínas Alimentares/metabolismo , Suplementos Nutricionais , Flatulência/metabolismo , Abrigo para Animais , Suínos/metabolismo , Poluição do Ar em Ambientes Fechados , Aminoácidos/administração & dosagem , Amônia/metabolismo , Animais , Dióxido de Carbono/metabolismo , Sulfeto de Hidrogênio/metabolismo , Masculino , Esterco/análise , Metano/metabolismo , Distribuição AleatóriaRESUMO
There is a lack of information regarding carbon dioxide (CO), methane (CH), and nitrous oxide (NO) emissions from pasture soils and the effects of grazing. The objective of this study was to quantify greenhouse gas (GHG) fluxes from pasture soils grazed with cow-calf pairs managed with different stocking rates and densities. The central hypothesis was that irrigated low-density stocking systems (SysB) would result in greater GHG emissions from pasture soils than nonirrigated high-density stocking systems (SysA) and grazing-exclusion (GRE) pasture sites. The nonirrigated high-density stocking systems consisted of 120 cow-calf pairs rotating on a total of 120 ha (stocking rate 1 cow/ha, stocking density 112,000 kg BW/ha, rest period of 60 to 90 d). The irrigated low-density stocking systems consisted of 64 cow-calf pairs rotating on a total of 26 ha of pasture (stocking rate 2.5 cows/ha, stocking density 32,700 kg BW/ha, rest period of 18 to 30 d). Both systems consisted of mixed cool-season grass-legume pastures. Static chambers were randomly placed for collection of CO, CH, and NO samples. Soil temperature (ST), ambient temperature (temperature inside the chamber; AT), and soil water content (WC) were monitored and considered explanatory variables for GHG emissions. GHG fluxes were monitored for 3 yr (2011 to 2013) at the beginning (P1) and at the end (P2) of the grazing season, always postgrazing. Paddock was the experimental unit (3 pseudoreplicates per treatment), and chambers (30 chambers per paddock) were considered multiple measurements of each experimental unit. A completely randomized design considered the term year × period as a repeated measure and chamber nested within paddock and treatment as the random term. Generally, SysB had greater CO emissions than SysA and GRE pasture sites across years and periods ( < 0.01). Soil temperature, AT, and WC had effects on CO emissions. Methane and NO emissions were observed from pasture sites of the 3 systems, but the effect of grazing was not constantly significant for CH and NO emissions. In addition, ST, AT, and WC did not conclusively explain CH and NO emissions. No clear trade-offs between GHG were observed; generally, GHG emissions increased from 2011 to 2013, which was likely associated with weather conditions, such as higher daily temperature and precipitation events. The central hypothesis that SysB would result in greater GHG emissions from pasture soils than SysA and GRE was not confirmed.
Assuntos
Dióxido de Carbono/química , Bovinos/fisiologia , Metano/química , Óxido Nitroso/química , Solo/química , Criação de Animais Domésticos , Animais , Feminino , Poaceae , Estações do Ano , Temperatura , Água , Tempo (Meteorologia)RESUMO
The objective of this study was to compare methane (CH) emissions from lactating beef cows grazed with different combinations of stocking rate and density. We hypothesized that a low stocking rate coupled with high-stocking-density grazing management would result in poorer forage quality, thereby increasing enteric CH emissions. System A (SysA) consisted of 120 cow-calf pairs rotating on a total of 120 ha divided into 2-ha pastures (stocking rate 1 cow/ha, stocking density 112,000 kg BW/ha, rest period of 60 to 90 d). System B (SysB) consisted of 16 groups of 4 cow-calf pairs each rotating on a 1.6-ha pasture (stocking rate 2.5 cows/ha, stocking density 32,000 kg BW/ha, rest period of 18 to 30 d). Enteric CH measurements were collected using a sulfur hexafluoride (SF) tracer gas method. Sampling occurred during 2012 and 2013 in 2 periods: the beginning (P1) and end of the grazing season (P2). Cannulated Angus cows were stratified by weight, age, and parity and were assigned to each treatment ( = 6) in a crossover design with a doubly repeated measures design, with period and day as repeated measures (α = 0.05). Dry matter intake was determined using chromic oxide (CrO) as a marker. Forage samples were collected ( = 3) for nutrient composition analyses and total forage mass determination. Forage botanical composition was determined using the dry-weight-rank method. Postgrazing herbage mass was greater for SysA during P2 in 2012 ( < 0.01) and 2013 ( = 0.01). Grasses were predominant and represented 67% to 96% of pastures; legumes contributed 3% to 21% of pastures across periods and treatments. The proportion of legumes tended to be higher in SysB pasture sites in P2 than in P1. There were no treatment effects on DMI. There was a period effect on DMI ( < 0.01); DMI of SysA and SysB cows increased from P1 to P2 (4 and 1.1 kg DMI/d increase, respectively). Cows ingested, on average, 2.6% (SysA) and 2.8% (SysB) of their BW. There was no year effect on CH emissions ( = 0.16). Daily enteric CH emissions did not vary with treatment and ranged from 195 to 249 g CH/d across treatment. Enteric CH emissions per unit GE intake varied with treatment during P1 (6.4% and 3.8% for SysA and SysB, respectively; < 0.01). Across treatments and periods, enteric CH emission per unit GE intake was 4.6%, which could be considered low for grazing lactating beef cows. It is likely that cows in the present study were selecting high-quality forage and produced comparatively lower CH emissions.
Assuntos
Criação de Animais Domésticos/métodos , Bovinos/fisiologia , Lactação/fisiologia , Metano/metabolismo , Ração Animal/análise , Animais , Peso Corporal , Dieta/veterinária , Ingestão de Alimentos , Feminino , Paridade , Poaceae , Gravidez , Estações do AnoRESUMO
The objective of this study was to determine the effect of diet CP levels on nitrous oxide (N2O), ammonia (NH3), and methane (CH4) emissions from 1) cattle housed in confined settings and 2) cattle manure following surface application to incubated soils. Twelve 500-kg Holstein steers were fed diets containing 10% CP (10CP) and 13% CP (13CP). The experimental design was a 2 × 2 Latin square conducted during two 20-d periods. Diets were fed for 14 d before each measurement period to allow for diet acclimation. Steers were housed in environmentally controlled rooms allowing for continuous emission measures of N2O, NH3, and CH4. At the end of the second period, manure was collected and surface applied to incubated soils to verify potential NH3 and N2O emissions. To assess emissions from incubated soils, 2 experiments were set up with 3 replicates each: Exp. 1, in which soil fertilization was based on manure mass (496 g of manure), and Exp. 2, in which soil fertilization was based on manure N content (targeted at 170 kg N/ha). Manure emissions were monitored for 7 d. Steers fed 13CP diets had increased daily NH3 emissions when compared to steers fed 10CP diets (32.36 vs. 11.82 ± 1.10 g NH3/d, respectively; P < 0.01). Daily N2O emissions from steers fed 13CP and 10CP diets were significantly different only during Period 1 (0.82 vs. 0.31 ± 0.24 g N2O/d; P = 0.04). Steers fed the 10CP diet had greater N2O emissions per unit of N consumed than steers fed the 13CP diet (9.73 vs. 4.26 ± 1.71 mg N2O/g N intake; P = 0.01). Diet CP levels did not affect enteric CH4 production from steers. In terms of soil emissions, different CP levels did not affect NH3, N2O, or CH4 emissions when soil fertilization was based on manure mass. However, NH3 emissions were reduced when manure from steers fed the 10CP diet was applied to soil based on N content. Ammonia emissions decreased during the 7-d incubation period. Conversely, N2O emissions increased over the period. Our results indicated that management of diet CP levels of confined finishing steers mitigates NH3 emissions from steers but does not affect enteric CH4. In addition, results suggested that soil characteristics might be as important as manure N content to generate NH3 and greenhouse gases from soils receiving manure fertilization.
Assuntos
Amônia/metabolismo , Bovinos/fisiologia , Proteínas Alimentares/farmacologia , Abrigo para Animais , Metano , Óxido Nitroso , Amônia/química , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Dieta/veterinária , Proteínas Alimentares/química , Masculino , Esterco , SoloRESUMO
Two experiments were conducted to study effects of dietary nitrate on enteric methane production, blood methemoglobin concentration, and growth rate in cattle. In Exp. 1, 36 Holstein steers (288 ± 25 kg BW) were fed increasing levels of dietary nitrate (6 levels; 0 to 3.0% of feed DM) in corn silage-based total mixed rations. Nitrate was introduced gradually in a 25-d adaptation period before methane production was determined in environmentally controlled rooms. In the rooms, feed intake was restricted and similar among all treatments. Methane production (g/d) decreased linearly as dietary nitrate concentration increased (P < 0.01). The apparent efficiency (measured methane reduction divided by potential methane reduction) with which enteric methane was mitigated was 49%. Blood methemoglobin levels increased with increasing nitrate dose. In Exp. 2, 300 Nelore bulls (392 ± 28 kg) were fed increasing levels of nitrate (6 levels; 0 to 2.4% of feed DM) in high-concentrate total mixed rations offered ad libitum. Feed intake decreased linearly with increasing level of dietary nitrate (P < 0.01). However, ADG was not affected by nitrate dose (P = 0.54), resulting in a linear improvement in G:F (P = 0.03) as dietary nitrate level increased. Carcass dressing percentage showed a quadratic response to incremental dietary nitrate, reaching the highest value at 0.96% of NO3/kg DM (P = 0.04).
Assuntos
Bovinos/fisiologia , Dieta/veterinária , Metano/metabolismo , Nitratos/farmacologia , Silagem/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Bovinos/crescimento & desenvolvimento , Masculino , Nitratos/administração & dosagemRESUMO
Literature on NH3 and H2S emissions from swine production facilities in North America was reviewed, and a meta-analysis was conducted on measured emissions data from swine houses and manure storage facilities as well as concentration data in the vicinity of swine production facilities. Results from more than 80 studies were compiled with results from the 11 swine sites in the National Air Emissions Monitoring Study (NAEMS). Data across studies were analyzed statistically using the MIXED procedures of SAS. The median emission rates from swine houses across various production stages and manure handling systems were 2.78 and 0.09 kg/yr per pig for NH3 and H2S, respectively. The median emission rates from swine storage facilities were 2.08 and 0.20 kg/yr per pig for NH3 and H2S, respectively. The size of swine farm that may trigger the need to report NH3 emissions under the Emergency Planning and Community Right-to-Know Act (EPCRA) is 3,410 pigs on the basis of the median NH3 emission rate (4.86 kg/yr per pig), but the threshold can be as low as 992 pigs on the basis of the 90th-percentile emission rates (16.71 kg/yr per pig). Swine hoop houses had significantly higher NH3 emission rate (14.80 kg/yr per pig) than other manure-handling systems (P < 0.01), whereas deep-pit houses had the highest H2S emission rate (16.03 kg/yr per pig, P = 0.03). Farrowing houses had the highest H2S emission rate (2.50 kg/yr per pig), followed by gestation houses, and finishing houses had the lowest H2S emission rate (P < 0.01). Regression models for NH3 and H2S emission rates were developed for finishing houses with deep pits, recharge pits, and lagoons. The NH3 emission rates increased with increasing air temperature, but effects of air temperature on H2S emission rates were not significant. The recharge interval of manure pits significantly affected H2S but not NH3 emission rates. The H2S emission rates were also influenced by the size of the operation. Although NH3 and H2S concentrations at the edge of swine houses or lagoons were often higher than corresponding acute or intermediate minimum risk levels (MRL), they decreased quickly to less than corresponding chronic or intermediate MRL as distances from emission sources increased. At the distances 30 to 1,185 m from emission sources, the average ambient concentrations for NH3 and H2S were 46 ± 46 µg/m(3) and 4.3 ± 8.6 µg/m(3) respectively.
Assuntos
Amônia/química , Abrigo para Animais , Sulfeto de Hidrogênio/química , Suínos/metabolismo , Amônia/metabolismo , Animais , Sulfeto de Hidrogênio/metabolismo , América do Norte , Eliminação de ResíduosRESUMO
The objective was to provide a systematic review of the literature on greenhouse gas (GHG) emissions from swine operations, with a meta-analysis that integrates results of independent studies. A total of 53 studies that measured GHG emissions from swine operations were included in the analyses. Results showed that the Intergovernmental Panel on Climate Change (IPCC) approaches were effective in estimating the overall CH4 and N2O emission levels from swine operations, but the variation of the measured emissions is not adequately captured. An overestimation by the IPCC approaches for CH4 emissions was observed for swine buildings with pit systems in European studies and the average percentage relative difference (PRD) between the measured and the IPCC values is -21.1%. The observed CH4 emissions from lagoons were lower than the IPCC estimated values and the average PRD is -33.9%. In North American studies the observed N2O emission factors for swine buildings with pit systems were significantly lower than the IPCC default values whereas in European studies they were significantly greater than the IPCC default values. The measured CH4 and N2O emissions were significantly affected by stage of production (P = 0.05 and <0.01, respectively) and geographic regions (P = 0.04 and 0.02, respectively). The IPCC approaches were effective in simulating the effect of temperature on CH4 emissions from outdoor slurry storage facilities whereas they could overestimate CH4 emissions from lagoons at low temperatures. The CH4 emissions from pits inside swine buildings were not significantly affected by average ambient temperatures. A positive relationship between diet CP content and CH4 emissions was confirmed in the meta-analysis. The obtained knowledge can be helpful in efforts to improve estimation of GHG emissions from swine operations.
Assuntos
Criação de Animais Domésticos , Mudança Climática , Metano/metabolismo , Óxido Nitroso/metabolismo , Suínos/fisiologia , Poluentes Atmosféricos/química , Poluentes Atmosféricos/metabolismo , Animais , Metano/química , Óxido Nitroso/químicaRESUMO
Dietary coconut oil (CNO) can reduce dry matter intake (DMI), enteric methane (eCH(4)) emissions, and milk fat yield of lactating cows. The goals of this research were to examine responses to different CNO concentrations during the habituation period (34-d) and to evaluate temporal patterns of DMI, eCH(4), and milk fat yield. Treatment diets contained (dry basis): 0.0% (CNO0), 1.3% (CNO1.3), 2.7% (CNO2.7), 3.3% (CNO3.3), or 4.0% CNO (CNO4). In experiment 1, 12 primi- or small secundiparous cows were housed in individual, environmentally controlled rooms and fed CNO0, CNO1.3, CNO2.7, or CNO4. Measurements included DMI, eCH(4), and milk yield and composition. Due to a precipitous drop in DMI (26%), cows fed CNO4 were replaced with cows fed CNO3.3 following d 10. Dietary CNO of 2.7% or more reduced eCH(4) emissions. Reduction was greater with increased CNO and during the first than the second half of the day. Simultaneously, decline in DMI of cows fed CNO2.7, CNO3.3, or CNO4 was increasingly precipitous with increased CNO concentration. Total-tract neutral detergent fiber (NDF) digestibility during wk 5 was reduced in cows fed CNO2.7 or CNO3.3, which in part explained concomitantly reduced eCH(4)/DMI. In addition, milk fat yield was depressed at an increasing rate in cows fed CNO2.7, CNO3.3, and CNO4. In experiment 2, DMI was measured individually in 12 multiparous cows during habituation to CNO0, CNO1.3, CNO2.7, or CNO3.3 for 21 d before relocation to individual, environmentally controlled rooms. Dietary CNO2.7 or CNO3.3 reduced DMI by d 4 and total-tract NDF digestibility during wk 5. Relocation to individual rooms was associated with a 15% reduction in DMI, which was not affected by treatment. Results showed that 2.7% or more dietary CNO reduced eCH(4) and DMI, caused milk fat depression, and decreased NDF digestibility.
Assuntos
Lactação/efeitos dos fármacos , Metano/biossíntese , Óleos de Plantas/farmacologia , Animais , Bovinos , Óleo de Coco , Dieta/veterinária , Fibras na Dieta/farmacologia , Ingestão de Alimentos/efeitos dos fármacos , Gorduras/análise , Feminino , Leite/químicaRESUMO
SUMMARY: The Kety-Schmidt technique provides quantitative measurement of whole-brain CBF. CBF is measured as the area between the arterial and venous washout curves of a diffusible tracer. Oxygen extraction and metabolism may be calculated from arterial and venous samples. In this report, we present a method for performing these measurements in an MR imaging environment. This technique could be useful for validation of MR imaging methods of hemodynamic and metabolic measurements in humans.
Assuntos
Algoritmos , Encéfalo/metabolismo , Angiografia por Ressonância Magnética/métodos , Óxido Nitroso/farmacocinética , Oximetria/métodos , Oxigênio/metabolismo , Animais , Encéfalo/anatomia & histologia , Simulação por Computador , Feminino , Humanos , Interpretação de Imagem Assistida por Computador/métodos , Macaca fascicularis , Masculino , Modelos Biológicos , Consumo de Oxigênio/fisiologia , Papio anubis , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
A series of experiments were conducted to quantify the effects of saponin extracts from Quillaja saponaria Molina (QS), Yucca schidigera Roezl ex Ortgies (YS), and Camellia sinensis (L.) Kuntze (TS) on gaseous emissions from steers (Bos taurus). During Exp. 1, a control diet [C1, corn (Zea mays L.) and corn silage basal diet] was compared with YS1 (C1 + 0.64% dietary DM of YS) and QS1 (C1 + 1.5% dietary DM of QS), with 4 replicates per treatment. During Exp. 2, the control diet (C2, corn and corn silage basal diet) was compared with TS2 (C2 + 0.25% dietary DM of TS). Product inclusion levels were established to provide the same concentration of saponin compounds across studies for Exp. 1 and 2. Experiment 3 compared C3 (corn and corn silage basal diet), QS3 (C3 + 1.5% QS), YS3 (C3 + 1.5% YS), and TS3 (C3 + 0.5% TS). Holstein steers (n = 12) at initial BW of 354 ± 10 kg (Exp. 1), 429 ± 10 kg (Exp. 2), 382 ± 16 kg (Period 1, Exp. 3) and 400 ± 12 kg (Period 2, Exp. 3) were individually housed in environmental rooms for 22 d per study. Gaseous emissions including methane (CH(4)), ammonia (NH(3)), and nitrous oxide (N(2)O) were monitored in room exhaust air. No differences in DMI (7.54 ± 0.09 kg) and ADG (1.16 ± 0.19 kg) were observed in Exp. 1 (P > 0.05). Adding TS2 to the diet improved DMI in Exp. 2 (8.94 kg in TS2 vs. 8.53 in C2; P < 0.01), whereas ADG was not affected by diet. During Exp. 3, steers fed the TS3 diet ate less (6.36 kg/d) and gained less BW (0.31 kg/d) compared with the other 3 treatments. Saponin inclusion did not alter daily CH(4) emission per unit DMI (13.17, 10.90, and 13.21 g/kg DMI, for Exp. 1, 2, and 3, respectively). Emissions of NH(3) per unit N intake were not affected by diets in Exp. 1 (134.89 mg/g N consumed) and Exp. 3 (134.99 mg/g N consumed). Feeding TS2 reduced NH(3) emission per unit of N consumed by 30% compared with C2 (P < 0.01). Feeding up to 0.5% of TS failed to reduce CH(4) emissions without impairing steer growth. Nitrous oxide emissions were not affected by TS addition. Air emissions were not affected by feeding steers with up to 1.5% YS. Feeding 1.5% QS to steers had an inconsistent effect upon NH(3) emissions and no other effects upon gaseous emissions were seen from steers.
Assuntos
Bovinos/fisiologia , Nitrogênio/química , Saponinas/farmacologia , Ração Animal , Fenômenos Fisiológicos da Nutrição Animal , Animais , Dieta/veterinária , Feminino , Nitrogênio/metabolismo , Plantas/química , Saponinas/químicaRESUMO
To determine if dietary medium-chain fatty acids (FA; C(8) to C(14)) may mitigate enteric methane emissions, 24 cows were blocked by body size (n=2) and randomly assigned to 1 sequence of dietary treatments. Diets were fed for 35 d each in 2 consecutive periods. Diets differed in concentrations of coconut oil (CNO; ~75% medium-chain FA): 0.0 (control) or 1.3, 2.7, or 3.3% CNO, dry matter basis. The control diet contained 50% forage (74% from corn silage), 16.5% crude protein (60% from rumen-degradable protein), 34% neutral detergent fiber (NDF; 71% from forage), and 28% starch, dry matter basis. Data and sample collections were from d 29 to 35 in environmentally controlled rooms to measure methane (CH(4)) production. Methane emitted was computed from the difference in concentrations of inlet and outlet air and flux as measured 8 times per day. Control cows emitted 464 g of CH(4)/d, consumed 22.9 kg of DM/d, and produced 34.8 kg of solids-corrected milk/d and 1.3 kg of milk fat/d. Treatment with 1.3, 2.7, or 3.3% dietary CNO reduced CH(4) (449, 291, and 253 g/d, respectively), but concomitantly depressed dry matter intake (21.4, 17.9, and 16.2 kg/d, respectively), solids-corrected milk yield (36.3, 28.4, and 26.8 kg/d, respectively), and milk fat yield (1.4, 0.9, and 0.9 kg/d, respectively). The amount of NDF digested in the total tract decreased with increased dietary CNO concentrations; thus, CH(4) emitted per unit of NDF digested rose from 118 to 128, 153, and 166 g/kg across CNO treatments. Dietary CNO did not significantly affect apparent digestibility of CP but increased apparent starch digestibility from 92 to 95%. No FA C(10) or shorter were detected in feces, and apparent digestibility decreased with increasing FA chain length. Coconut oil concentrations of 2.7 or 3.3% decreased yields of milk FA
Assuntos
Lactação/efeitos dos fármacos , Metano/biossíntese , Óleos de Plantas/farmacologia , Ração Animal , Animais , Bovinos , Óleo de Coco , Dieta/veterinária , Relação Dose-Resposta a Droga , Ingestão de Alimentos , FemininoRESUMO
Effects of dietary adipic acid (0 vs. 1%) and corn dried distillers grains with solubles (DDGS; 0 vs. 20%) were evaluated on hen performance and egg characteristics from 26 to 34 wk of age. Four isocaloric and isonitrogenous diets were randomly assigned to blocks of 6 consecutive cages (36 cages per diet; 2 hens per cage). On wk 2 and 7 of the experiment, excreta were collected by cage block, mixed, and equally split into 2 containers. Sodium bisulfate (SBS) was spread (8.8 kg/100 m(2)) on the top surface of half of the containers. All containers were stored uncovered for 14 d at room temperature. Excreta pH, DM, and N content were measured on d 0, 7, and 14 of storage. Feed intake (112 g/d per hen), egg production (96.1%), and egg specific gravity (1.079 g/g) were not affected by diet. On excreta collection day, a synergy (P = 0.014) between dietary adipic acid and DDGS was detected, as the lowest excreta pH was obtained with the diet including both adipic acid and DDGS. On d 7 of storage, excreta pH was still reduced by dietary adipic acid (P = 0.046) and DDGS (P < 0.001), but a week later, only dietary DDGS decreased excreta pH (8.91 vs. 9.21; P < 0.001). Whereas dietary adipic acid had no influence on excreta N loss, excreta from hens fed 20% DDGS lost 19.7% more N (P = 0.039) during storage than hens not eating DDGS. Surface amendment of excreta with SBS increased excreta DM content, with the effect being even more marked on d 14 of storage (increase of 6.7 percentage units; P < 0.001), consistently decreased excreta pH during storage (P < 0.001) and reduced N loss by 26.1% for the 14 d of storage period.
Assuntos
Adipatos/farmacologia , Galinhas/fisiologia , Fezes/química , Nitrogênio/metabolismo , Sulfatos/química , Zea mays , Ração Animal , Fenômenos Fisiológicos da Nutrição Animal , Animais , Dieta/veterinária , Ovos/normas , Feminino , Concentração de Íons de Hidrogênio , Oviposição/efeitos dos fármacos , Água/química , Aumento de PesoRESUMO
OBJECTIVE: To determine whether unihemispheral hemodynamic failure is independently associated with cognitive impairment among participants in the National Institute of Neurological Disorders and Stroke-sponsored, multicenter, randomized clinical trial, Randomized Evaluation of Carotid Occlusion and Neurocognition (RECON). METHODS: Forty-three patients were randomized into RECON after recent symptomatic carotid artery occlusion and asymmetrically increased oxygen extraction fraction (OEF) by PET (OEF ratio >1.13), indicating stage II hemodynamic failure on the side of occlusion. The PET-positive patients were compared with 28 RECON-enrolled patients who met all clinical and radiographic inclusion/exclusion criteria but had no OEF asymmetry. A multivariable regression compared patients with PET OEF >1.13 or ≤1.13, stratifying by TIA vs. stroke as the qualifying event. The dependent variable was a composite neurocognitive score derived from averaging age-normalized z scores on a test battery that included global and internal carotid artery (ICA) side-relevant hemisphere-specific tests. RESULTS: There were no differences in demographic, clinical, or radiologic characteristics between the PET-positive and PET-negative patients except for PET OEF asymmetry. The unadjusted average neurocognitive z score was -1.45 for the PET-positive and -1.25 for the PET-negative patients, indicating cognitive impairment in both groups but no difference between them (p = 0.641). After adjustment for age, education, side of occlusion, depression, and previous stroke, there was a significant difference between PET-positive and PET-negative patients among those with TIA as a qualifying event (average z score = -1.41 vs. -0.76, p = 0.040). Older age and right ICA side were also significant in this model. CONCLUSION: Hemodynamic failure is independently associated with cognitive impairment in patients with carotid occlusion. This finding establishes the physiologic parameter upon which the extracranial-intracranial bypass will be tested.
Assuntos
Circulação Cerebrovascular , Transtornos Cognitivos/fisiopatologia , Transtornos Cognitivos/psicologia , Idoso , Doenças das Artérias Carótidas/complicações , Doenças das Artérias Carótidas/fisiopatologia , Doenças das Artérias Carótidas/psicologia , Transtornos Cognitivos/diagnóstico por imagem , Estudos de Coortes , Escolaridade , Feminino , Lateralidade Funcional , Humanos , Ataque Isquêmico Transitório/diagnóstico por imagem , Ataque Isquêmico Transitório/fisiopatologia , Ataque Isquêmico Transitório/psicologia , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Destreza Motora , Testes Neuropsicológicos , Tomografia por Emissão de Pósitrons , Acidente Vascular Cerebral/complicações , Acidente Vascular Cerebral/diagnóstico por imagem , Acidente Vascular Cerebral/psicologia , Teste de Sequência Alfanumérica , Percepção Visual , Testes de Associação de PalavrasRESUMO
TBA, or 2,4,6-tribromoanisole, is a musty-smelling metabolite of 2,4,6-tribromophenol that is used as a flame retardant and an antifungal agent for wooden pallets and packaging materials. The compound can impart its peculiar, often offensive, odor on product packaging to the concern of consumers for the safety of the package contents. These studies were conducted to evaluate the safety of TBA to humans ingesting products tainted with TBA. In addition to the 28-day oral study, a bacterial reverse mutation study was conducted, and confirmed that TBA was not mutagenic. To evaluate oral safety, TBA was evaluated in single dose and 5-day and 4-week repeated dose oral toxicity studies in rats. The test article, administered in single gavage doses of 2000, 5000 and 7500 mg/kg body weight (bw), in 5 daily repeated doses of 1000, 2000 or 3000 mg/kg bw/day or in 28 daily oral gavage doses of 0 001, 0.01, 100, and 1000 mg/kg bw/day did not result in any deaths. Also, the single and repeat dose studies resulted in no significant differences between control and treated groups on body weight gain, food consumption, clinical observations, blood biochemical values, and hematology findings. Treatment-related adverse findings were only detected in male rats during repeated dose studies and were associated with high plasma concentrations of TBA. The test article-related finding of hyaline droplets in the cortical tubular epithelium of kidneys was associated with increases in α(2 µ)-globulin content in the kidneys as indicated by the intensity of immunohistochemical staining. These findings were correlated with an increased weight of kidneys in males administered 1000mg/kgbw/day for 28days. Chemical induction of hyaline droplets containing α(2µ)-globulin in the renal proximal tubule is a process unique to the male rat and is not relevant for human risk assessment. Findings of increased liver weight with minimal centrilobular to diffuse hepatocellular hypertrophy in males treated with TBA at 1000mg/kg bw/day for 28days were considered to be an adaptive metabolic response to xenobiotic administration. The increased volume of urine, noted in both males and females treated with 1000mg/kg bw/day was considered adaptive and necessary to excrete the high xenobiotic burden resulting from TBA administration. TBA appeared to be highly bioavailable since high concentrations of TBA were detected in plasma, at 1, 4 and 8h after administration of TBA at 100 and 1000mg/kg bw for 1 and 28days. Levels were dose-related but did not clarify the course of TBA elimination with time after administration. These studies indicate that TBA, administered orally to rats, produced male rat-specific, treatment-related toxicity at the highest orally administered dose in repeated dose (5-day at 3000mg/kg bw and 28-day at 1000mg/kg bw) studies. Therefore, the NOAEL for the 28-day oral study was determined to be 1000mg/kg bw/day for the rat.