RESUMO
The n-hexane extract of Houttuynia cordata was shown to inhibit prostaglandin synthase in vitro. Phytochemical examination led to the identification of five fatty acids (linolenic, linoleic, oleic, palmitic and stearic), cepharanone B, phytol and stigmast-4-ene-3,6-dione. The inhibitory effect of the extract on prostaglandin formation in vitro could be attributed mainly to linoleic and linolenic acid.
RESUMO
Several extracts of Hyptis verticillata and isolated compounds were evaluated for their anti-inflammatory, antibacterial, antisecretory, and cytotoxic properties. The aerial parts yielded (R)-5-hydroxypyrrolidin-2-one and essential oil with the main components alpha-pinene, beta-pinene, and thymol. Spectroscopic methods (UV, IR, 1H-NMR, 13C-NMR, mass, CD) fully characterized (R)-5-hydroxypyrrolidin-2-one, as it was isolated by a bioassay guided fractionation. The essential oil, (R)-5-hydroxypyrrolidin-2-one, as well as the previously isolated rosmarinic acid and dehydropodophyllotoxine contributed to the antibacterial effects of H. verticillata. Furthermore, rosmarinic acid showed significant capillary stabilizing effects. Sideritoflavone inhibited prostaglandin synthase to a significant extent and had antisecretory effects comparable to those of NPPB. The cytotoxicity of the aqueous extract, as demonstrated using KB and HT 29 cell lines, may be of toxicological relevance in cases of internal application.
Assuntos
Anti-Infecciosos/isolamento & purificação , Anti-Inflamatórios não Esteroides/isolamento & purificação , Antineoplásicos/isolamento & purificação , Inibidores de Ciclo-Oxigenase/isolamento & purificação , Plantas Medicinais/química , Pirrolidinonas/isolamento & purificação , Animais , Anti-Infecciosos/farmacologia , Anti-Inflamatórios não Esteroides/farmacologia , Antineoplásicos/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Embrião de Galinha , Colo , Inibidores de Ciclo-Oxigenase/farmacologia , Humanos , Técnicas In Vitro , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/metabolismo , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Pirrolidinonas/farmacologia , Coelhos , Espectrofotometria , Células Tumorais CultivadasRESUMO
Polyunsaturated alkamides isolated from Achillea species, Echinacea angustifolia DC., Anacyclus pyrethrum (L.) Link, and Aaronsohnia pubescens (Desf.) Bremer & Humphries, (Compositae) were shown to possess inhibitory activity in in vitro cyclooxygenase (sheep seminal microsomes) and 5-lipoxygenase (porcine leukocytes) assays. Activity appeared to depend on the particular structure of the alkamides.
