RESUMO
Background: Teeth, one of the strongest structures of the human body, stands as reliable evidence in forensic odontology. The gender and age can be estimated from a tooth using various methods. We aimed to estimate the calcium content of an intact tooth and compare it with its average weight and dimension and also to find the possible correlation with the age and gender of an individual. Materials and Methods: A total of 180 extracted teeth were divided into group A (21-40), group B (41-60) and group C (61-80). Calcium estimation by decalcification followed by ethylene diamine tetra-acetic acid method analysis was performed. Results: Greatest calcium values were found in the males with mandibular premolars in group A, of maxillary premolars and mandibular molars in group B, as well as of maxillary canine, maxillary molars and mandibular incisors in group C. Greater calcium levels in the age of group B and C in mandibular incisors and male maxillary molars and in the jaws of group B, group C incisors and group C canines with P < 0.05. Calcium content and the weight of the teeth decreased with ageing. Conclusion: Calcium levels significantly varied in each tooth type, arch, gender and age.
RESUMO
Introduction: Immunohistochemistry (IHC) has not always been an easy field for the research beginners like postgraduates, research fellows and scientists. Meaningful interpretation of IHC positivity needs expertise. This could be made easier for beginners by developing a conceptual framework of markers. The literature review revealed a lack of qualitative evidence on the hitherto IHC studies on oral diseases about the overall expression of IHC markers and its comparison with pathology and normal tissues. Aim: This scoping review aimed to examine the literature and classify the various immunohistochemistry markers of oral diseases based on the tissue, cell and site of expression. Materials and Methods: The review was in accordance with Preferred Reporting Items for scoping reviews (PRISMA -ScR). Electronic databases such as PubMed and Cochrane were searched for relevant articles till 2021. Results: We included 43 articles. We found five different possibilities of the site of expression of a marker in a cell. They are the nucleus, cytoplasm, cell membrane, extracellular matrix or any of the above combinations. Based on the tissue of expression, we also mapped the markers expressed in oral diseases to their tissue of origin as ectoderm, endoderm, mesoderm and markers with multiple tissues of expression. Based on our results, we derived two classifications that give an overview of the expression of IHC markers in oral diseases. Conclusion: This scoping review derived new insight into the classification of IHC markers based on cell lineage, tissue and site of expression. This would enable a beginner to better understand a marker with its application and the interpretation of the staining in research. This could also serve as a beginner's guide for any researcher to thrive and explore the IHC world.
