RESUMO
There is an emerging need for the development of new anticancer nanocomposite which exhibits imaging properties and targeted drug delivery. In the present study, a nanobiocomposite was prepared, in this direction, which contains carbon nanoparticles (CNP), methotrexate (Mtx) and asparaginase (Asp) coupled with fluorescein isothiocyanate (FITC). The prepared nanobiocomposite kills only the cancer cells due to the presence of Mtx which is a folic acid analogue and targets the cancer cells due to the over expression of folate receptors on their surface and apoptosis occurs due to the anticancer activity of enzyme asparaginase. The results obtained from the present study confirmed the sustained release of Mtx and Asp from the nanobiocomposite. The nanobiocomposite was found to be haemocompatible, biocompatible and showed more than 90% apoptosis. The drug pathway was clearly monitored due to the presence of FITC in the nanobiocomposite. This study proves the effectiveness of the fabricated nanoconjugate, as it helps in imaging, targeting and destructing the cancerous cells. The prepared nanoconjugate may be effectively applied in in vivo experiments before applying on to humans.
Assuntos
Antineoplásicos/administração & dosagem , Asparaginase/administração & dosagem , Carbono/química , Portadores de Fármacos/química , Transportadores de Ácido Fólico/metabolismo , Metotrexato/administração & dosagem , Nanopartículas/química , Células 3T3 , Animais , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Asparaginase/farmacologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Liberação Controlada de Fármacos , Hemólise/efeitos dos fármacos , Humanos , Metotrexato/farmacologia , Camundongos , Microscopia de Força Atômica , Nanocompostos/administração & dosagem , Nanocompostos/química , Espectroscopia de Infravermelho com Transformada de Fourier , Propriedades de Superfície/efeitos dos fármacosRESUMO
The modification of carbon nanoparticles (CNPs) using biological molecules is important in the field of chemical biology, as the CNPs have the potential to deliver the drugs directly to the targeted cells and tissues. We have modified the CNPs by coating bovine serum albumin (BSA) on their surfaces and loaded with methotrexate (Mtx). Infrared spectra have revealed the coating of BSA and Mtx on CNP (CBM). Scanning electron microscopy (SEM) and atomic force microscope (AFM) pictures have exhibited the spherical nature of the composite and coating of the proteins on CNPs. The prepared CBM biocomposite has exhibited a sustained release of drug. MTT assay using A549 lung cancer cell lines has revealed 83% cell death at 150 µg/ml concentration of CBM. These results indicate that CNPs based biocomposites may be tried as therapeutic agents in treatment of cancer like diseases.
