Hydrodynamic cavitation-assisted acid pretreatment and fed-batch simultaneous saccharification and co-fermentation for ethanol production from sugarcane bagasse using immobilized cells of Scheffersomyces parashehatae.

A new alternative for hydrodynamic cavitation-assisted pretreatment of sugarcane bagasse was proposed, along with a simultaneous saccharification and co-fermentation (SSCF) process performed in interconnected columns. Influential variables in the pretreatment were evaluated using a statistical design, indicating that an ozone flow rate of 10 mg min-1 and a pH of 5.10 resulted in 86 % and 72 % glucan and xylan hydrolysis yields, respectively, in the subsequent enzymatic hydrolysis process. Under these optimized conditions, iron sulfate (15 mg L-1) was added to assess Fenton pretreatment, resulting in glucan and xylan hydrolysis yields of 92 % and 71 %, respectively, in a material pretreated for 10 min. In SSCF, ethanol volumetric productivities of 0.33 g L-1 h-1 and of 0.54 g L-1 h-1 were obtained in batch and fed-batch operation modes, achieving 26 g L-1 of ethanol in 48 h in the latter mode.

[Prevalence of myasthenia gravis in Colombia]. / Prevalencia de la miastenia grave en Colombia.

TITLE: Prevalencia de la miastenia grave en Colombia.

A review on recent developments in hydrodynamic cavitation and advanced oxidative processes for pretreatment of lignocellulosic materials.

Environmental problems due to utilization of fossil-derived materials for energy and chemical generation has prompted the use of renewable alternative sources, such as lignocellulose biomass (LB). Indeed, the production of biomolecules and biofuels from LB is among the most important current research topics aiming to development a sustainable bioeconomy. Yet, the industrial use of LB is limited by the recalcitrance of biomass, which impairs the hydrolysis of the carbohydrate fractions. Hydrodynamic cavitation (HC) and Advanced Oxidative Processes (AOPs) has been proposed as innovative pretreatment strategies aiming to reduce process time and chemical inputs. Therefore, the underlying mechanisms, procedural strategies, influence on biomass structure, and research gaps were critically discussed in this review. The performed discussion can contribute to future developments, giving a wide overview of the main involved aspects.

Pretreatment of sugarcane bagasse using hydrodynamic cavitation technology: Semi-continuous and continuous process.

Development of new technologies for pretreatment of lignocellulosic biomass is a current research challenge. In this way, hydrodynamic cavitation (HC) was used to assist alkaline hydrogen peroxide pretreatment of sugarcane bagasse (SCB) in sequential batches (SB-HC), semi-continuous (SC-HC) and continuous (C-HC) processes. Pretreatment resulted in compositional modifications in the material, mainly regarding the cellulose and lignin contents. The released sugars after enzymatic hydrolysis resulted, on average, in 42 g and 32-35 g of glucose per 100 g of SCB for samples treated in B-HC (10 min of process) and SC-HC process (7.5 min residence time), respectively. In C-HC process, with an average residence time of 7.5 min and 3.75 min, 38-46 g and 32-38 g of glucose per 100 g of SCB were obtained respectively in enzymatic hydrolysis step. HC technology was shown as a promising alternative for pretreatment of lignocellulosic biomass in all evaluated configurations aiming to produce high value bioproducts.

A new multiplex PCR for differential identification of Shigella flexneri and Shigella sonnei and detection of Shigella virulence determinants.

Most of the multiplex PCR (mPCR) used to identify Shigella do not discriminate between Shigella species or serotypes. We designed a mPCR to differentiate between S. flexneri and S. sonnei strains based on the detection of markers associated with the she pathogenicity island described in Shigella. In addition, specific primers were included to detect the Shigella virulence determinants ShET-1 and ShET-2 enterotoxin genes. The analysis of 304 Shigella strains from Chile and 79 Shigella strains from other geographic locations indicated that the mPCR described here detected all Shigella species and specifically differentiated S. flexneri and S. sonnei. The technique was sensitive, reproducible, specific and simple to perform, providing a new tool with the potential to be employed for epidemiological and diagnostic purposes.