RESUMO
OBJECTIVE: To evaluate the expression of a set of miRNAs to identify differentially expressed miRNAs that might be considered reliable biomarkers on Diabetic Retinopathy (DR) blood samples. RESULTS: Expression levels of MiR-320a, MiR-342-3p, MiR-155, MiR-99a, MiR-29a and MiR-27b were analyzed in 60 healthy controls, 48 Diabetes Melitus (DM) without DR patients and 62 DR patients by qRT-PCR. MiR-320a was shown to be downregulated in the plasma of DR patients compared with DM patients without DR and healthy subjects. Target genes were predicted using miRWalk3.0, miR targeting data and target gene interaction data were imported to Cytoscape to visualize and merge networks and top ranked predicted genes were run through Ontology Genes to perform enrichment analysis on gene sets and classification system to identify biological processes and reactome pathways associated with DR. Highly scored target genes of miR-320a were categorized for various biological processes, including negative regulation of cell aging, negative regulation of cellular protein metabolic process and regulation of cellular response to stress that are critical to the development of DR. Our findings suggest that MiR-320a may have a role in the pathogenesis of DR and may represent novel biomarkers for this disease.
Assuntos
Retinopatia Diabética/sangue , Retinopatia Diabética/genética , MicroRNAs/sangue , Biomarcadores/sangue , MicroRNA Circulante/sangue , MicroRNA Circulante/genética , Regulação para Baixo , Feminino , Perfilação da Expressão Gênica , Regulação da Expressão Gênica/genética , Ontologia Genética , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase em Tempo Real , Transdução de Sinais/genéticaRESUMO
Diabetic Retinopathy, the main cause of visual loss and blindness among working population, is a complication of Diabetes mellitus (DM), which has been described as a major public health challenge, so it is important to identify biomarkers to predict and to stratify patient´s possibility for developing DR. MicroRNAs (miRNAs) are small non-coding RNA molecules that have showed to be promising disease biomarkers and association of miRNAs with the possibility to develop DR has been reported. However, evaluating miRNA expression involves normalization of RT-qPCR data using internal reference genes that should be properly determined, considering their impact on expression levels calculation and, until date, there is no unanimity on reference miRNAs for the investigation of circulating miRNAs in DR. We aimed to estimate the appropriateness of a group of miRNAs as normalizers to identify which might be considered steady internal reference genes in expression studies on DR plasma samples. Expression levels of candidates were analyzed in 60 healthy controls, 48 DM without DR patients and 62 DR patients with two statistical tools: NormFinder and RefFinder. MiR-328-3p was the most stable gene and we also investigated the effect of gene normalization, demonstrating that different normalization strategies have important implications for accurate data interpretation.
Assuntos
Retinopatia Diabética/genética , MicroRNAs/genética , Adulto , Idoso , Biomarcadores/sangue , Estudos de Casos e Controles , MicroRNA Circulante/sangue , MicroRNA Circulante/genética , Diabetes Mellitus/sangue , Diabetes Mellitus/genética , Retinopatia Diabética/sangue , Feminino , Perfilação da Expressão Gênica , Marcadores Genéticos , Humanos , Masculino , MicroRNAs/sangue , Pessoa de Meia-Idade , Estabilidade de RNA , Reação em Cadeia da Polimerase em Tempo RealRESUMO
Objetivo: descrever o perfil dos resultados das Ultrassonografias Transfontanelas. Método: estudo descritivo, de abordagem quantitativa, retrospectivo, no levantamento documental, no livro de registro de exames de USGT realizadas nos recém-nascidos. Os dados foram apresentados em figuras. Resultados: o estudo contemplou 52 prontuários de RN. Quanto ao diagnóstico ultrassonográfico, 63% dos RNs apresentaram resultado normal. As USGT anormais (37%) foram classificadas em cinco tipos de injúrias: Hemorragia Intracraniana (HIC) e Hemorragia Peri intraventricular (HPIV) perfazendo 9% dos afetados, Encefalopatia Hipoxico-isquêmica (EHI, incluindo Leucomalácia Periventricular) com 17%, Dilatação ventricular (DV) com 9%e Malformações cerebrais com 2% da população. Conclusão: foi possível neste estudo identificar as doenças mais prevalentes diagnosticadas pelo exame de USGT. Esses dados são de grande valia pelo seu poder norteador na prática da assistência ao paciente recém-nascido.
