RESUMO
Renal cell carcinoma (RCC) are frequently chemo- and radiation resistant. Thus, there is a need for identifying biological features of these cells that could serve as alternative therapeutic targets. We performed suppression subtractive hybridization (SSH) on patient-matched normal renal and RCC tissue to identify variably regulated genes. 11 genes were strongly up-regulated or selectively expressed in more than one RCC tissue or cell line. Screening of filters containing cancer-related cDNAs confirmed overexpression of 3 of these genes and 3 additional genes were identified. These 14 differentially expressed genes, only 6 of which have previously been associated with RCC, are related to tumour growth/survival (EGFR, cyclin D1, insulin-like growth factor-binding protein-1 and a MLRQ sub-unit homologue of the NADH:ubiquinone oxidoreductase complex), angiogenesis (vascular endothelial growth factor, endothelial PAS domain protein-1, ceruloplasmin, angiopoietin-related protein 2) and cell adhesion/motility (protocadherin 2, cadherin 6, autotaxin, vimentin, lysyl oxidase and semaphorin G). Since some of these genes were overexpressed in 80-90% of RCC tissues, it is important to evaluate their suitability as therapeutic targets.
Assuntos
Biomarcadores Tumorais/análise , Carcinoma de Células Renais/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias Renais/genética , Northern Blotting , Adesão Celular , Sobrevivência Celular , Primers do DNA , DNA de Neoplasias/genética , Humanos , Neovascularização Patológica , Hibridização de Ácido Nucleico , Análise de Sequência com Séries de Oligonucleotídeos , Reação em Cadeia da Polimerase , Células Tumorais CultivadasRESUMO
We investigated the potential of carbon-11 choline positron emission tomography (PET) for the detection of lymph node and bone metastases in prostate cancer. A total of 23 patients were studied (known metastases: 8; suspicion of metastases: 3; primary staging: 12). Whole-body PET imaging was performed 5 min after injection of the tracer and completed within 1 h. Focally increased tracer uptake in bone or abdominal lymph node regions was interpreted as representing tumour involvement. All known bone and lymph node metastases could be recognized by [11C]choline PET. One out of ten negative scans for primary staging was false-negative (lymph node <1 cm) and one out of two positive scans was false-positive with regard to lymph node involvement (focal bowel activity). It is concluded that [11C]choline PET is a promising new tool for the primary staging of prostate cancer, with lymph node and bone metastases demonstrating high tracer uptake. Therapeutic management could be influenced by these results in that the technique may permit avoidance of surgical lymph node exploration.
Assuntos
Radioisótopos de Carbono , Colina/farmacocinética , Neoplasias da Próstata/diagnóstico por imagem , Tomografia Computadorizada de Emissão , Idoso , Humanos , Metástase Linfática , Masculino , Pessoa de Meia-IdadeRESUMO
Supported by irisfluorescein angiography it should be examined if systolic ciliary pressure found by oculooscillodynamography is to be seen in every part of the uvea. Iris vessels were found to be perfused at 42 mm Hg (Median, Q1-Q3 = 38-45 mm Hg) whereas oculooscillodynamography displayed systolic ciliary pressure to be 69 mm Hg (62-72 mm Hg). This different blood pressure in different districts of the uvea may be one explanation for the particular vulnerability of iris vessels e.g. in ischemic ophthalmopathy.
