Multi-Faceted Bioactivity Assessment of an Exopolysaccharide from Limosilactobacillus fermentum NCDC400: Antioxidant, Antibacterial, and Immunomodulatory Proficiencies.

Exopolysaccharides (EPS) are acknowledged for their diverse functional and technological properties. This study presents the characterization of EPS400, an acidic exopolysaccharide sourced from the native probiotic Limosilactobacillus fermentum NCDC400. Notably, this strain has demonstrated previous capabilities in enhancing dairy food texture and displaying in vivo hypocholesterolemic activity. Our investigation aimed to unveil EPS400's potential biological roles, encompassing antioxidant, antibacterial, and immunomodulatory activities. The results underscore EPS400's prowess in scavenging radicals, including the 2,2-diphenyl-1-picrylhydrazyl radical, 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid) radical, superoxide radical, hydroxyl radical, and chelating activity targeting the ferrous ion. Furthermore, EPS400 displayed substantial antibacterial effectiveness against prevalent food spoilage bacteria such as Pseudomonas aeruginosa NCDC105 and Micrococcus luteus. Remarkably, EPS400 exhibited the ability to modulate cytokine production, downregulating pro-inflammatory cytokines TNF-α, IL-1ß, IL-6, and nitric oxide, while concurrently promoting the release of anti-inflammatory cytokine IL-10 within lipopolysaccharide-activated murine primary macrophages. Additionally, EPS400 significantly (p ≤ 0.05) enhanced the phagocytic potential of macrophages. Collectively, our findings spotlight EPS400 as a promising contender endowed with significant antioxidant, antibacterial, and immunomodulatory attributes. These characteristics propose EPS400 as a potential pharmaceutical or bioactive component, with potential applications in the realm of functional food development.

Effect of clove bud and curry leaf essential oils on the anti-oxidative and anti-microbial activity of burfi, a milk-based confection.

With scientific advancement in the field of food science and technology, there has been an increased availability of innovative ingredients that can be utilized towards value addition, quality enhancement, natural preservation, shelf life enhancement and adding novelty to traditional Indian dairy products. Here a scientific attempt has been made to increase the anti-oxidative and anti-microbial potential of burfi, a popular confection of Indian sub-continent with low shelf life using herbal essential oils (EOs) (natural preservative, antioxidant and antimicrobial) such as curry leaf (CRYF) (0.05-0.15 ppm) and clove bud (CLVB) (0.15-0.25 ppm) EO. Samples were subjected to physico-chemical, sensory, anti-oxidant and microbiological analysis and the results revealed that increasing the herbal EOs levels in burfi led to increase in anti-microbial and anti-oxidative attributes but simultaneously decreased the sensory attributes. Physico-chemical attributes remained unaffected upon EOs incorporation. Principal component analysis revealed 81.5% relation between the burfi samples and its quality attributes (DPPH activity, ABTS activity, total phenolic content, sensory attributes, standard plate count, yeast and mould count, moisture content, water activity, lightness, L* value, redness, a* value and yellowness, b* value). Herbal EOs i.e. CRYF@0.10 ppm and CLVB@0.20 ppm on khoa basis were found optimum for incorporation into burfi for enhancing storage stability without compromising the sensory acceptability.

Optimization of heat treatment and curcumin level for the preparation of anti-oxidant rich ghee from fermented buffalo cream by Central Composite Rotatable Design.

Adverse health effects of synthetic anti-oxidants have necessitated the use of natural anti-oxidants in food products. However, their incorporation may result into undesirable changes in physico-chemical and sensory attributes of the product. Hence, the present investigation was undertaken to prepare anti-oxidant rich ghee using curcumin (yellow pigment found in turmeric) as the natural anti-oxidant. Effects of varying curcumin levels (160-350 ppm), heating temperature (110-120 °C) and duration (16-22 min) on anti-oxidant, chemical and sensory attributes of ghee were studied. Increasing level of curcumin significantly increased the DPPH free radical scavenging activity and decreased the amount of conjugated dienes formation. Increasing heating time and temperature significantly decreased the anti-oxidant activity in ghee, but their combination significantly increased the activity. Increase in curcumin level and heating temperature improved the sensory attributes of ghee, but longer duration of heating decreased the same. Optimization using Central Composite Rotatable Design yielded 350 ppm of curcumin and heat treatment of 115 °C for 17.89 min for most acceptable, anti-oxidant rich ghee with a desirability value of 0.966. The model developed was found to predict the product characteristics adequately.