Taeniasis Saginata as Unexpected Cause in the Case of a Woman Scared of Getting Pregnant.

This reported case occurred in non-endemic taeniasis area, and neither the patient nor her family members had habit of eating raw beef. So previously there was no suspicion of taeniasis, moreover the main complaint in the case of a 24-yr-old Javanese woman living in Surabaya, East Java, Indonesia, was that she wished to get pregnant. She scared of getting pregnant, because she felt weak and unfit which was not pretty good for fetus that will be conceived. Other complaint was only sometimes diarrhea. Laboratory test showed eosinophilia (12%). It caused suspicion of intestinal parasitic infection. The examination of stool specimen showed gravid proglottids and the eggs were identified from Taenia saginata tapeworm. Treatment with albendazole successfully expelled the adult worm, and as days passed by, she felt better and healthier, and after seven months we found her to be pregnant.

Immunoglobulin subclass in experimental murine Toxocara cati infection.

AIM: The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human. MATERIALS AND METHODS: The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial. RESULTS: The specific IgG subclass in mice infected with T. cati mice was found to be IgG2ß. CONCLUSION: Subclass of IgG, especially IgG2ß, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.

Honey can repairing damage of liver tissue due to protein energy malnutrition through induction of endogenous stem cells.

AIM: This study was to evaluate effect of honey in repairing damage of liver tissue due to energy protein malnutrition and in mobilization of endogenous stem cells. MATERIALS AND METHODS: Male mice model of degenerative liver was obtained through food fasting but still have drinking water for 5 days. It caused energy protein malnutrition and damage of liver tissue. The administration of 50% (v/v) honey was performed for 10 consecutive days, while the positive control group was fasted and not given honey and the negative control not fasted and without honey. Observations of regeneration the liver tissue based on histologically examination, observation of Hsp70 expression, and homing signal based on vascular endothelial growth factor-1 (VEGF-1) expression using immunohistochemistry technique. Observation on expression of CD34 and CD45 as the marker of auto mobilization of hematopoietic stem cells using flow cytometry technique. RESULTS: There is regeneration of the liver tissue due to protein energy malnutrition, decrease of Hsp70 expression, increase of VEGF-1 expression, and high expression of CD34 and CD45. CONCLUSION: Honey can improve the liver tissue based on: (1) Mobilization of endogenous stem cells (CD34 and CD45); (2) Hsp70 and VEGF-1 expressions as regeneration marker of improvement, and (3) regeneration histologically of liver tissue.

Honeybee product therapeutic as stem cells homing for ovary failure.

AIM: Complexity of the method of isolation, cultivation in vitro and the expensive cost of transplantation process of stem cells, it would require an innovation to homing and differentiation of stem cells and increase folliculogenesis. The stem cells homing was achieved through the provision of food or beverages derived from natural materials like honeybee product. Through honeybee product, there will be homing of stem cells and accompany with the sources from the body itself will take place in regeneration of the ovary. MATERIALS AND METHODS: Female rats model of degenerative ovary was obtained through food fasting but still have drinking water for 5 days. It caused malnutrition and damage of the ovarian tissue. The administration of 50% honeybee product (T1) was performed for 10 consecutive days, while the positive control group (T0+) was fasted and not given honeybee product and the negative control (T0-) not fasted and without honeybee product. Observations were taken for homing of stem cells, raised of folliculogenesis, differentiation of stem cells, and regeneration of the ovarian tissue using routine H&E staining. RESULTS: Homing of stem cells shown the vascular endothelial growth factor and granulocyte colony-stimulating factor expression; enhancement of folliculogenesis was indicated by an increase of follicle dee Graaf count; enhancement of differentiation of stem cells was indicated by growth differentiation factor-9 expression; and regeneration of ovarian tissue indicated by intact ovarian tissue with growing follicles. CONCLUSION: Honeybee product can be induced endogenous stem cells in regeneration of ovary failure due to malnutrition.