Antifungal Efficacy of Spice Extracts against Candida albicans: An in vitro study.

BACKGROUND: Candida species are normal commensals and are isolated intra-orally in 17%-75% of healthy individuals and all debilitated people. Eradication of candidiasis is complicated by the emergence of Candida strains that are resistant to the currently used antifungal agents. Plants as remedies are gaining popularity in developed countries. Although many plants have already been investigated against Candida albicans, the search is still to find a long-term prevention or cure for oral candidiasis. OBJECTIVES: The objective of this study was (1) to evaluate the antifungal activity of black pepper, bay leaf, cinnamon, and cumin against C. albicans and (2) to determine the minimum inhibitory concentration (MIC) of spice extracts against C. albicans. MATERIALS AND METHODS: Spices obtained from the local market were dried and powdered. Solvent extracts were obtained by maceration with methanol followed by filtration and evaporation. The antifungal efficacy was assessed using cup-plate diffusion method followed by the determination of MIC by serial tube dilution technique. Statistical analysis was done using one-way analysis of variance followed by Tukey's post hoc test. P < 0.05 was considered as statistically significant. RESULTS: All the extracts evaluated showed variable degree of inhibition zones against C. albicans with cinnamon showing the highest inhibition (49.3 ± 0.52) mm and also with least MIC against C. albicans (<0.05 mg/ml). CONCLUSION: These results exhibit the antifungal activity of the spice extracts against C. albicans, which may be useful in the treatment of oral candidiasis.

Effect of Mouthrinses containing Olive Oil, Fluoride, and Their Combination on Enamel Erosion: An in vitro Study.

AIM: The aim of the study was to evaluate the effect of mouth-rinses containing olive oil, fluoride, and their combination on enamel erosion. MATERIALS AND METHODS: An in vitro study of 45 enamel specimens, which were prepared from 45 extracted teeth, was employed. Each specimen was subjected to 10 alternative demineralization and remineralization cycles. Remineralizing cycle includes 5 minutes exposure with one of the mouthrinse, and demineralizing cycle includes 3 minute exposure to 1% citric acid. Mean surface roughness (Ra) was measured from surfometry before and after cycles. Statistical tests used were Student's unpaired t-test and one-way analysis of variance (ANOVA), followed by Tukey's post hoc test. RESULTS: Among the three mouthrinses, Listerine and Xerostom showed maximum protection against erosion on enamel. The 2% olive oil mouthrinse showed the least protection against erosion on enamel. CONCLUSION: The study indicated that the Listerine and Xerostom mouthrinses are valuable preventive measures. CLINICAL SIGNIFICANCE: Mouthrinses are effective for lessening erosive demineralization and in aggregating remineralization of tooth surfaces which are the important factors to prevent enamel erosion.

Knowledge and Attitude about HIV/AIDS among HIV-Positive Individuals in Davangere.

A cross-sectional study was carried out on 100 HIV patients (55 male and 45 female) age 21 years and older registered at the Antiretroviral Therapy Centre of Chigateri General Hospital, Davangere, Karnataka, India, to evaluate their knowledge and attitude toward HIV. Overall, the source of knowledge about AIDS was through newspaper or television, 38% were aware of their AIDS status, 61% disclosed their status to family, 86% had incorrect knowledge about transmission, 57% experienced no moral support, 62% felt stigmatized, 52% discriminated against, 44% were denied treatment, 57% felt isolated, 54% experienced difficulty in carrying out daily activity, 52% of patients' social and personal life was affected, and 41% felt shy when disclosing their disease. This data reveals the need to consider the basic knowledge of the HIV patients about HIV/AIDS.

Comparison of Impact Strength and Fracture Morphology of Different Heat Cure Denture Acrylic Resins: An In vitro Study.

BACKGROUND: The fracture of acrylic resin denture is rather common occurrence and causes inconvenience to the patients. This study was carried out to evaluate and compare the impact strength and fracture morphology of four different heat cure acrylic materials. MATERIALS AND METHODS: Acrylic resin specimens were prepared using preformed metal die of dimension 65 × 10 × 3 mm. The specimens were finished, polished and subjected to impact strength evaluation using impact testing machine. The loads at which the specimens fracture are recorded and subjected to statistical analysis. Fracture surface analysis was done. Macroscopic analysis was performed by visual inspection of the fractured surfaces using a stereoscopic microscope. About 5 mm sections of all the fragments were subjected to scanning electron microscopy for microscopic analysis to verify fracture morphology. RESULTS: Mean values of the impact strength were compared by statistical methods. The impact strength data were subjected to variance homogeneity tests. Fracture surface analysis data was analyzed by statistical methods. The mean impact strength of Lucitone 199 was higher than Acrylyn-H, DPI Heat cure & Trevalon. CONCLUSION: Within the limitations of this study, it was concluded that the impact strength of the acrylic resins is affected by the reinforcement of fibers. Increased intermediate fractures increased impact strength. Brittle fractures morphology showed fewer undercuts and clearer surface. Intermediate fractures morphology showed more undercuts than clear surfaces.

The antimicrobial activity of the three commercially available intense sweeteners against common periodontal pathogens: an in vitro study.

AIM: To evaluate the in vitro antimicrobial activity of three commercially available intense sweeteners against two common periodontal pathogens Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. MATERIALS AND METHODS: Three commercially available intense sweeteners namely saccharin, aspartame and sucralose were obtained and powdered. Necessary concentrations of the sweeteners were prepared by mixing them with an inert solvent. The antimicrobial efficacy was assessed using agar well diffusion technique. Statistical analysis was done using one-way ANOVA followed by Tukey's post hoc test. p-value < 0.05 was considered statistically significant. RESULTS: All the three sweeteners showed significant antimicrobial activity against the periodontal pathogens tested. Sucralose containing sucralose showed maximum zone of inhibition, against Aggregatibacter actinomycetemcomitans. Saccharin and aspartame containing saccharin and aspartame respectively, showed maximum zone of inhibition, against Porphyromonas gingivalis. CONCLUSION: All the sweeteners used in this study have demonstrated significant antimicrobial activity. Therefore, these sweeteners could be recommended as an ideal alternative to sucrose. CLINICAL SIGNIFICANCE: Dental caries and periodontal diseases are ubiquitous diseases of mankind caused by microorganisms. Dental caries is caused by sucrose. By altering the source like intense sweetener we can combat caries as well as with its antimicrobial properties against periodontopathic bacteria, we can reduce prevalence of periodontal diseases.

The efficacy of antiseptic mouth rinses in comparison with dental floss in controlling interproximal gingivitis.

OBJECTIVES: Maintaining good oral hygiene is important to combat periodontal diseases. The use of tooth brush alone does not serve the purpose, especially in inaccessible areas like proximal embrasures, which demand the use of some adjuncts like proximal cleaning aids. Hence, the objective of this study was to compare the clinical efficacy of two antimicrobial mouth rinses (Cool mint Listerine and 0.2% Chlorhexidine gluconate) with dental floss in reducing interproximal gingivitis and dental plaque in an unsupervised condition. MATERIALS AND METHODS: A randomized, controlled, single-blind (observer), parallel-group clinical trial in accordance with the ADA guidelines was conducted for a period of 6 months. Four index age groups (12, 15, 35-44, and 65-74 years) were divided into four groups, i.e., brushing, brushing and flossing, brushing and rinsing with Listerine, and brushing and rinsing with Chlorhexidine, so that each group comprised 40 subjects. Interproximal gingivitis and dental plaque were assessed using Modified Gingival Index, Turesky-Gilmore-Glickman modified Quigley-Hein Plaque Index and Gingival Bleeding Index. Analysis of variance (ANOVA) was used for multiple group comparisons, followed by Tukey's post hoc for group-wise comparisons. RESULTS: Chlorhexidine and Listerine showed significant reduction in plaque and gingivitis level compared to others, the activity of Chlorhexidine being more significant. CONCLUSIONS: The level of interproximal gingivitis control efficacy provided by the Listerine and Chlorhexidine was "at least as good as" that provided by the dental floss. Hence, they can be recommended for the patients with gingivitis as an adjunctive to the usual home care routine.

A peer group approach model of oral health promotion among orphans at Puduchery, South India.

BACKGROUND: Interactions between oral and systemic health are bi-directional and complex, involving many pathways. The orphans have been sympathized with, ignored, vitrified or even hidden away in the community. Hence, providing health care services for orphans remains a challenge. Oral health education has a positive impact in lowering plaque and gingivitis scores in health educational programs. OBJECTIVE: The objective of this study is to assess the effectiveness of peer group health promotion model among 11 to 16 year old orphans at Puduchery, South India. MATERIALS AND METHODS: A clinical trial of 6 months duration was carried out among 72 orphans with age ranging from 11 to 16 years residing in Cluny Padmini Sneha Illam, at Puduchery, India. The baseline data regarding oral health and oral hygiene practices were obtained using a pre-tested close-ended questionnaire. Oral health education was provided to 4 randomly picked children who in turn provided the same to their peer group orphans, supervised by the investigator. Tooth brushes and fluoridated tooth pastes were provided to all the participants throughout the study period. Oral hygiene of the participants was assessed using Modified Plaque Index by Loe H and Gingival Index by Loe H and Silness at baseline, 3(rd) and 6(th) month interval. Paired Student t test was used to analyze the categorical data. RESULTS: Majority of the respondents felt consuming sugar was not harmful for dental health, while 15 respondents were not sure about the outcome. When asked if oral hygiene was important for general health, 45.8% were not aware of its association. Although all the participants brushed their teeth daily, none of them reported the use of dental floss. The findings of this study indicated a statisticallysignificant lower mean plaque score of 0.54 ± 0.20 at 6(th) month when compared to the baseline score of 1.76 ± 0.24. Similarly, statistically significant lower gingival index score of 0.65 ± 0.11 at 6(th) month was observed when compared to the baseline score of 1.76 ± 0.24. CONCLUSIONS: The results of this study indicate that oral hygiene of orphans was improved using a peer group approach model of oral health promotion.

Fluoride and bacterial content of bottled drinking water versus municipal tap water.

BACKGROUND: Water is a divine gift. People quench their thirst without questioning the source of water. But, apprehension about contaminants in municipal water supplies along with increased fear of fluorosis made bottled drinking water as one of the important tradable commodities. OBJECTIVES: The objectives of the study were to determine and compare the fluoride and bacterial contents of commercially available bottled drinking water and municipal tap water in Davangere city, Karnataka. MATERIALS AND METHODS: Fifty samples of 10 categories of bottled drinking water with different batch numbers were purchased and municipal water from different sources were collected. Fluoride levels were determined by an ion-selective electrode. Water was cultured quantitatively and levels of bacteria were calculated as colony-forming units (CFUs) per milliliter. RESULTS: Descriptive analysis of water samples for fluoride concentration was in the range of 0.07-0.33 for bottled drinking water, Bisleri showing the highest of 0.33. A comparison of the mean values of microbial count for bottled drinking water with that of municipal tap water showed no statistically significant difference, but was more than the standard levels along with the presence of fungus and maggots. CONCLUSION: The fluoride concentration was below the optimal level for both municipal tap water and bottled drinking water. CFUs were more than the recommended level in both municipal tap water and bottled drinking water.

Fluoride content in bottled drinking waters, carbonated soft drinks and fruit juices in Davangere city, India.

BACKGROUND: The regular ingestion of fluoride lowers the prevalence of dental caries. The total daily intake of fluoride for optimal dental health should be 0.05-0.07 mg fluoride/kg body weight and to avoid the risk of dental fluorosis, the daily intake should not exceed a daily level of 0.10 mg fluoride/kg body weight. The main source of fluoride is from drinking water and other beverages. As in other countries, consumption of bottled water, juices and carbonated beverages has increased in our country. OBJECTIVE: To analyze the fluoride content in bottled water, juices and carbonated soft drinks that were commonly available in Davangere city. MATERIALS AND METHODS: Three samples of 10 commercially available brands of bottled drinking water, 12 fruit juices and 12 carbonated soft drinks were purchased. Bottled water and carbonated soft drinks were stored at a cold place until fluoride analysis was performed and a clear juice was prepared using different fruits without the addition of water. Then, the fluoride analysis was performed. RESULTS: The mean and standard deviation of fluoride content of bottled water, fruit juices and carbonated soft drinks were measured, which were found to be 0.20 mg (±0.19) F/L, 0.29 mg (±0.06) F/L and 0.22 mg (±0.05) F/L, respectively. CONCLUSION: In viewing the results of the present study, it can be concluded that regulation of the optimal range of fluoride in bottled drinking water, carbonated soft drinks and fruit juices should be drawn for the Indian scenario.

Antifungal efficacy of Punica granatum, Acacia nilotica, Cuminum cyminum and Foeniculum vulgare on Candida albicans: an in vitro study.

BACKGROUND: The establishment and maintenance of oral microbiota is related not only to interbacterial coaggregations but also to interactions of these bacteria with yeasts. Hence, it is important for agents used in the treatment of oral diseases to have antifungal properties for effective therapy. OBJECTIVE: The main purpose of this study was to evaluate the in vitro antifungal efficacy of Punica granatum, Acacia nilotica, Cuminum cyminum and Foeniculum vulgare on Candida albicans. MATERIALS AND METHODS: The pomegranate peel is separated, dried and powdered. Fennel, cumin and acacia bark obtained from the tree are powdered. Candida is inoculated at 37˚C and seeded on Sabourauds agar medium. Sterilized filter papers saturated with 30 µl of the extracts are placed on the seeded plates and inoculated at 24 and 48 h. Zones of inhibition on all four sides are measured around the filter paper with a vernier caliper. The experiments were repeated on four plates, with four samples of each extract on one plate for all of the extracts. RESULTS: All the above-mentioned ingredients showed antifungal property, with Punica granatum showing the highest inhibition of Candida albicans with a mean zone of inhibition of 22 mm. P-values <0.05 were obtained for Punica granatum when compared with the other extracts. CONCLUSION: The results showed the potential use of these products as cheap and convenient adjuvants to pharmaceutical antifungal products.

Prevalence and severity of dental fluorosis among 13- to 15-year-old school children of an area known for endemic fluorosis: Nalgonda district of Andhra Pradesh.

OBJECTIVES: The present study was conducted to assess dental fluorosis and to compare fluorosis in incisor teeth among 13- to 15-year-old school children of Nalgonda district, Andhra Pradesh. METHODS: Cross-sectional analytical study was conducted. A total of 1000 school children aged 13 to 15 years were selected by stratified cluster sampling from 4 different areas with different levels of naturally occurring fluoride in drinking water. Fluorosis was recorded using TF index (TFI). RESULTS: Prevalence of fluorosis (TFI score, > or = 1) was 100% at all the 4 different fluoride levels. The prevalence and severity increased with increased fluoride levels in drinking water. Prevalence and severity of fluorosis did not show any significant variation between maxillary and mandibular incisor teeth. CONCLUSION: There is a high prevalence of mild-to-moderate fluorosis in Nalgonda district, even in areas with optimal fluoride levels in water.

Assessment of atmospheric microbial contamination in a mobile dental unit.

INTRODUCTION: Bioaerosols are important considerations in infection control as well as in occupational health. Bioaerosols may carry potentially hazardous microbes, viruses, fungi, allergens, and other toxic substances that may harm the dental operator, patient, and the dental assistant by causing nosocomial infections. OBJECTIVE: To assess the level of atmospheric microbial contamination before, during, and after dental treatment procedures in the dental operatory of a mobile dental unit (MDU). MATERIALS AND METHODS: The study included three treatment sessions on different working days, with an interval of one month. The MDU was fumigated before the start of the study. Brain Heart Infusion Agar with 5% sheep blood was used to collect the gravitometric settling of aerosols produced before, during, and after dental treatment procedures. The agar plates were sent for aerobic and anaerobic culture. RESULTS: The results showed that atmospheric microbial contamination (CFUs/plate) was 4 times higher during working sessions as compared to the levels before the working sessions. At the end of the working day, aerosols decreased by almost 3 times that seen during work. CONCLUSION: The aerosols increased during and after work sessions. This shows the increased risk of transmission of infectious agents to the dentists who work in the MDU. Hence, all necessary preventive measures should be advised and need to be followed strictly.

The effect of mango and neem extract on four organisms causing dental caries: Streptococcus mutans, Streptococcus salivavius, Streptococcus mitis, and Streptococcus sanguis: an in vitro study.

BACKGROUND AND OBJECTIVES: Chewing twigs of the mango or neem tree is a common way of cleaning the teeth in the rural and semi-urban population. These twigs are also believed to possess medicinal properties. The present study was conducted to evaluate the antimicrobial effects of these chewing sticks on the microorganisms Streptococcus mutans , Streptococcus salivarius , Streptococcus mitis , and Streptococcus sanguis which are involved in the development of dental caries. An additional objective was to identify an inexpensive, simple, and effective method of preventing and controlling dental caries. MATERIALS AND METHODS: The sticks were sun dried, ground into a coarse powder, and weighed into 5 gm, 10 gm, and 50 gm amounts. These were added to 100 ml of deionized distilled water. After soaking for 48 h at 4 degrees C, the water was filtered. The filtrate was inoculated onto blood agar plates containing individual species of microorganisms and incubated at 37 degrees C for 48 h. RESULTS: Mango extract, at 50% concentration, showed maximum zone of inhibition on Streptococcus mitis . Neem extract produced the maximum zone of inhibition on Streptococcus mutans at 50% concentration. Even at 5% concentration neem extract showed some inhibition of growth for all the four species of organisms. INTERPRETATION AND CONCLUSION: A combination of neem and mango chewing sticks may provide the maximum benefit. We recommend the use of both the chewing sticks.