RESUMO
The results from a comparative study of some methods for extraction of Rubella haemagglutinin from infected BHK-21 cells are presented. The best haemagglutinin extraction was achieved after the cells were treated with Tween 80-ether. The combined application of alkaline extraction and Tween 80-ether treatment also gave good results.
Assuntos
Hemaglutininas Virais/isolamento & purificação , Vírus da Rubéola/imunologia , Rubéola (Sarampo Alemão)/microbiologia , Animais , Linhagem Celular , Células Cultivadas/microbiologia , Cricetinae , Estudos de Avaliação como Assunto , Testes de Hemaglutinação , Inoculações Seriadas , Virologia/métodosRESUMO
The incorporation of labelled precursors of DNA, RNA and proteins in F. cells infected with Coxsackie B4 virus has been traced both in the presence and absence of total histone from nucleated erythrocytes during the infectious process. In comparison with the virus infection alone, the exogenous histone supressed the incorporation of 3H-thymidine in early hours and the incorporation of 3H-uridine and 14C-leucine in the 9th hour after virus infection. The established repression of the incorporation of the labelled precurssors for RNA and proteins correlates with a drop in the order of 90 to 99% in the yield of infectious virus.
