Effects of macrophytes on ecosystem metabolism and net nutrient uptake in a groundwater fed lowland river.

Transport and transformation of inorganic nutrients are influenced by abiotic-biotic interactions and determine downstream water quality. Macrophytes play an important role in these complex ecological interactions. The role of macrophytes was studied in three reaches of the groundwater-fed, oligotrophic River Fischa with different macrophyte coverage and biomass. This was done by measuring metabolism and calculating changes in nutrient loading and concentrations, which were determined via an upstream-downstream mass balance approach. As the dominant autotrophs, we expected macrophytes (i) to have a direct effect by uptake and release, and (ii) an indirect effect by slowing down flow, which results in changed sedimentation patterns and altered conditions for heterotrophic microbial organisms implicating higher turnover and uptake rates. The seasonal development of macrophytes in 2017 had a strong impact on gross primary production, but not on ecosystem respiration. Increase in macrophyte biomass led to higher GPP (max. 5.4 g O2m-2d-1). ER was highest in autumn in the reach with intermediate macrophyte biomass (max. 10.1 g O2m-2d-1). We observed that the autotrophic uptake of phosphorus accounted for 80-145% of the P-PO4-flux and concluded that P-uptake by macrophytes from the sediment is an important source of phosphate for macrophytes in the river. By accumulating fine sediment, macrophytes are improving the availability of phosphate for their own long-term development. N-NO3, represented >99% of the nitrogen flux. N-NO3 net uptake was higher in the reaches with more macrophytes (0.84 vs. 0.12 g m-2d-1), but in average only 21% of the net uptake could be related to autotrophic nitrogen uptake in the reach with high macrophyte biomass. Dissimilatory uptake by heterotrophic organisms, most probably denitrification, were of high relevance. Macrophytes supported microbial uptake and release by improving conditions and slowing down flow. In the River Fischa, an oligotrophic river with low variability of environmental parameters, macrophytes greatly affected nutrient uptake by direct and indirect pathways.

Influence of water temperature and water depth on macrophyte-bacterioplankton interaction in a groundwater-fed river.

Biotic interactions shape the community structure and function of ecosystems and thus play an important role in ecosystem management and restoration. To investigate how water temperature (related to the season) and water depth (related to spatial patterns of river morphology) affect macrophyte-bacterioplankton interactions in a groundwater-fed river, we conducted the structural equation modeling on datasets grouped by hydrological conditions. In addition to direct effects on macrophyte growth and/or bacterioplankton development, water temperature and water depth could both regulate the role of different nutrients (inorganic and organic) on affecting these biological indicators. Deeper water depth intensified the positive relationship between macrophytes and bacterioplankton, while higher temperature switched the relationship from being positive to negative. Our study provides empirical evidences that abiotic variables, even with relatively low fluctuations, play a critical role in regulating the patterns and strengths of interaction between macrophytes and bacterioplankton.

Aquatic methane dynamics in a human-impacted river-floodplain of the Danube.

River-floodplain systems are characterized by changing hydrological connectivity and variability of resources delivered to floodplain water bodies. Although the importance of hydrological events has been recognized, the effect of flooding on CH4 concentrations and emissions from European, human-impacted river-floodplains is largely unknown. This study evaluates aquatic concentrations and emissions of CH4 from a highly modified, yet partly restored river-floodplain system of the Danube near Vienna (Austria). We covered a broad range of hydrological conditions, including a 1-yr flood event in 2012 and a 100-yr flood in 2013. Our findings demonstrate that river-floodplain waters were supersaturated with CH4, hence always serving as a source of CH4 to the atmosphere. Hydrologically isolated habitats in general have higher concentrations and produce higher fluxes despite lower physically defined velocities. During surface connection, however, CH4 is exported from the floodplain to the river, suggesting that the main channel serves as an "exhaust pipe" for the floodplain. This mechanism was especially important during the 100-yr flood, when a clear pulse of CH4 was flushed from the floodplain with surface floodwaters. Our results emphasize the importance of floods differing in magnitude for methane evasion from river-floodplains; 34% more CH4 was emitted from the entire system during the year with the 100-yr flood compared to a hydrologically "normal" year. Compared to the main river channel, semiisolated floodplain waters were particularly strong sources of CH4. Our findings also imply that the predicted increased frequency of extreme flooding events will have significant consequences for methane emission from river-floodplain systems.

Current status and restoration options for floodplains along the Danube River.

Floodplains are key ecosystems of riverine landscapes and provide a multitude of ecosystem services. In most of the large river systems worldwide, a tremendous reduction of floodplain area has occurred in the last 100 years and this loss continues due to pressures such as land use change, river regulation, and dam construction. In the Danube River Basin, the extent of floodplains has been reduced by 68% compared to their pre-regulation area, with the highest losses occurring in the Upper Danube and the lowest in the Danube Delta. In this paper, we illustrate the restoration potential of floodplains along the Danube and its major tributaries. Via two case studies in the Upper and Lower Danube, we demonstrate the effects of restoration measures on the river ecosystem, addressing different drivers, pressures, and opportunities in these regions. The potential area for floodplain restoration based on land use and hydromorphological characteristics amounts to 8102 km(2) for the whole Danube River, of which estimated 75% have a high restoration potential. A comparison of floodplain status and options for restoration in the Upper and Lower Danube shows clear differences in drivers and pressures, but certain common options apply in both sections if the local context of stakeholders and societal needs are considered. New approaches to flood protection using natural water retention measures offer increased opportunities for floodplain restoration, but conflicting societal needs and legal frameworks may restrict implementation. Emerging issues such as climate change and invasive non-native species will need careful consideration in future restoration planning to minimize unintended effects and to increase the resilience of floodplains to these and other pressures.

Impact of drying and re-flooding of sediment on phosphorus dynamics of river-floodplain systems.

One of the consequences of human impacts on floodplains is a change in sedimentation leading to enhanced floodplain aggradation. Thus, accumulated sediments rich in nutrients might interfere with floodplain restoration. In this study we investigated the phosphorus release behavior of sediments from shallow backwaters of an isolated floodplain of the Danube River situated east of the city of Vienna with the aim to understand the effects of changes in dry/wet cycles on established floodplain sediments. In the light of restoration plans aiming at increased surface water exchange with the river main channel, the response of sediments to frequent alternations between desiccation and inundation periods is a key issue as changes of sediment properties are expected to affect phosphorus release. In order to determine the effect of changing hydrological conditions on internal phosphorus loading, we exposed sediments to different dry/wet treatments in a laboratory experiment. Total phosphorus (TP) release from sediments into the water column increased with increasing duration of dry periods prior to re-wetting. Partial correlation analysis showed significant positive correlations between ΔTP and ΔNH(4)(+) as well as between ΔTP and ΔFe(3+) concentrations (Δ refers to the difference between the final and initial concentration during the wetting period), indicating that enhanced mineralization rates leading to a concomitant release of NH(4)(+) and TP and the reduction of iron hydroxides leading to a concomitant release of Fe(3+) and TP are the mechanisms responsible for the rise in TP. Repeated drying and wetting resulted in elevated phosphorus release. This effect was more pronounced when drying periods led to an 80% reduction in water content, indicating that the degree of drying is a major determinant controlling phosphorus release upon re-wetting. The reconnection of isolated floodplains will favor fluctuating hydrologic conditions and is therefore expected to initially lead to high rates of phosphorus release from sediments.

Sources and composition of organic matter for bacterial growth in a large European river floodplain system (Danube, Austria).

Dissolved and particulate organic matter (DOM and POM) distribution, lignin phenol signatures, bulk elemental compositions, fluorescence indices and microbial plankton (algae, bacteria, viruses) in a temperate river floodplain system were monitored from January to November 2003. We aimed to elucidate the sources and compositions of allochthonous and autochthonous organic matter (OM) in the main channel and a representative backwater in relation to the hydrological regime. Additionally, bacterial secondary production was measured to evaluate the impact of organic carbon source on heterotrophic prokaryotic productivity. OM properties in the backwater tended to diverge from those in the main channel during phases without surface water connectivity; this was likely enhanced due to the exceptionally low river discharge in 2003. The terrestrial OM in this river floodplain system was largely derived from angiosperm leaves and grasses, as indicated by the lignin phenol composition. The lignin signatures exhibited significant seasonal changes, comparable to the seasonality of plankton-derived material. Microbially-derived material contributed significantly to POM and DOM, especially during periods of low discharge. High rates of bacterial secondary production (up to 135 µg C L(-1) d(-1)) followed algal blooms and suggested that autochthonous OM significantly supported heterotrophic microbial productivity.