Assuntos
Proteínas de Bactérias/genética , Análise Mutacional de DNA , RNA Polimerases Dirigidas por DNA/genética , Farmacorresistência Bacteriana Múltipla/genética , Testes de Sensibilidade Microbiana , Mutação , Mycobacterium tuberculosis/genética , Testes Imediatos , Tuberculose Resistente a Múltiplos Medicamentos/diagnóstico , Antibióticos Antituberculose/uso terapêutico , Genótipo , Humanos , Mycobacterium tuberculosis/efeitos dos fármacos , Mycobacterium tuberculosis/patogenicidade , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Rifampina/uso terapêutico , Tuberculose Resistente a Múltiplos Medicamentos/tratamento farmacológico , Tuberculose Resistente a Múltiplos Medicamentos/microbiologiaRESUMO
The production of porous cross-linked enzyme aggregates (p-CLEAs) is a simple and effective methodology for laccase immobilization. A three-phase partitioning technique was applied to co-precipitate laccase and starch, followed by cross-linking with glutaraldehyde and removal of starch by α-amylase to create pores in the CLEAs. Scanning electron microscopy revealed a very smooth spherical structure with numerous large pores. The half-life of free laccase at 55°C was calculated to be 1.3h, while p-CLEAs did not lose any activity even after 14 h. p-CLEAs also exhibited improved storage stability, catalytic efficiency and could be recycled 15 times with 60% loss of activity. The catalysts decolorized triphenylmethane and reactive dyes by 60-70% at initial dye concentrations of 2 and 0.5 g L(-1), respectively, without any mediators. These results suggest the potential of CLEA technology in waste-water treatment.
Assuntos
Basidiomycota/enzimologia , Cor , Corantes/química , Lacase/síntese química , Compostos de Terfenil/química , Compostos de Tritil/química , Corantes/isolamento & purificação , Reagentes de Ligações Cruzadas/química , Enzimas Imobilizadas/síntese química , Porosidade , Compostos de Terfenil/isolamento & purificação , Compostos de Tritil/isolamento & purificaçãoRESUMO
Evidence is presented showing that the synthesis of the estrogen receptor activation factor (E-RAF) in the rat uterus is under both estrogenic and progestational control. An ELISA method for the measurement of E-RAF is described and used to measure the E-RAF titre in the pregnant rat uterus. It is proposed that E-RAF synthesis during pregnancy is under the exclusive control of progesterone.
