RESUMO
Histopathology demonstrates disruption of the basal layer of the epidermis in lichen planus (LP) and altered expression of basement membrane zone (BMZ) components occurs in cutaneous and oral LP. This is the first study in erosive LP of the vulva to investigate the expression of components of the BMZ and extracellular matrix by indirect immunofluorescence. Six biopsies from lesional vulval erosive LP were compared with two biopsies from normal vulva and five biopsies from normal skin. In erosive vulval LP there was widespread disruption of several BMZ components compared to normal skin. The hemidesmosome antigens were disrupted and attenuated, or absent. Expression of lamina lucida proteins and anchoring filaments also showed some alteration. Lamina densa components were altered and in particular there was very marked thickening, streaking and fragmentation of the anchoring fibrils. Some dermal extracellular matrix proteins were increased. This study has demonstrated widespread damage to the BMZ in erosive LP of the vulva, in particular the hemidesmosomes (alpha6beta4 integrin, BP230, BP180) and anchoring fibrils (collagen VII). This suggests an alteration in antigenic expression in the BMZ that may lead to exposure of epitopes and thus make these proteins vulnerable to attack by autoantibodies.
Assuntos
Matriz Extracelular/imunologia , Hemidesmossomos/imunologia , Líquen Plano/imunologia , Pele/imunologia , Doenças da Vulva/imunologia , Autoantígenos/metabolismo , Autoimunidade , Membrana Basal/imunologia , Membrana Basal/metabolismo , Membrana Basal/patologia , Moléculas de Adesão Celular/metabolismo , Colágeno Tipo VII/metabolismo , Matriz Extracelular/metabolismo , Feminino , Hemidesmossomos/metabolismo , Humanos , Laminina/metabolismo , Líquen Plano/metabolismo , Líquen Plano/patologia , Pele/metabolismo , Doenças da Vulva/metabolismo , Doenças da Vulva/patologia , CalininaRESUMO
After bilateral or subtotal nephrectomy male rats were given intraperitoneal injections of the plasticiser di-2-ethylhexylphthalate (DEHP) at differing time intervals in doses that do not affect the liver of normal rats. 200mg DEHP/100g body weight had no influence on the parameters measured. After administering 7 x 200mg/100g body weight for 14 days, liver weight and in vitro activity of the mixed function oxidation system increased significantly. In addition, the hexobarbital sleeping time decreased, indicating a stimulation of the activity of drug metabolising enzymes in vivo. Similar results were obtained after the administration of 56 x 50mg DEHP/100g body weight for 19 weeks. It is concluded that uraemic rats are more susceptible to inducing effects, but that the DEHP toxicity is of little importance.
