RESUMO
Orthotopic liver transplantation is the only effective form of therapy currently available for patients with fulminant hepatic failure (FHF). The use of an extracorporeal (EC) liver assist device (LAD) may result in improved presurgical clinical management. Alternatively, patients treated with LADs could avoid the transplantation procedure if they are able to regenerate a critical mass of hepatocytes that will sustain functional viability. In this study, the efficacy of a prototype hollow fiber LAD seeded with rabbit hepatocytes was assessed in vivo by the use of two different animal models: (1) normal rabbits injected with diazepam or lidocaine, and (2) a galactosamine (Gal)-intoxicated rabbit model of FHF. The EC LAD clearly decreased the blood levels of the two drugs and significantly generated diazepam and lidocaine metabolites indicating the maintenance of active P450 forms in the cellular component of the devices. A 6-hour EC treatment significantly increased the survival time and delayed the onset of hepatic encephalopathy (HE) in the Gal-intoxicated rabbits. Histological evaluations of postmortem livers showed greater hepatocyte regenerative activity in the animals treated with hepatocyte-seeded LADs than in the two control groups, e.g., rabbits not treated or treated with unseeded devices. These findings support the concept that a microporous hollow fiber LAD seeded with rabbit hepatocytes is able to sustain drug detoxification in vivo as well as to modify the course of FHF in a well-characterized animal model.
Assuntos
Órgãos Artificiais , Circulação Extracorpórea , Encefalopatia Hepática/terapia , Fígado , Animais , Diazepam/metabolismo , Estudos de Avaliação como Assunto , Galactosamina , Encefalopatia Hepática/induzido quimicamente , Encefalopatia Hepática/patologia , Lidocaína/metabolismo , Masculino , CoelhosRESUMO
Intravenous anaesthesia with a combination of ketamine (Ketalar) and midazolam (Dormicum) infusions was administered to a 49-year-old patient, with a documented history of opiate allergy, undergoing coronary artery bypass surgery. A ketamine infusion supplemented with indomethacin suppositories was used for postoperative pain control.
Assuntos
Anestesia Intravenosa , Ponte de Artéria Coronária , Ketamina , Midazolam , Hipersensibilidade a Drogas , Humanos , Cuidados Intraoperatórios , Ketamina/administração & dosagem , Masculino , Midazolam/administração & dosagem , Pessoa de Meia-Idade , Dor Pós-OperatóriaAssuntos
Anestesia Geral/efeitos adversos , Halotano/efeitos adversos , Hipertermia Maligna/etiologia , Óxido Nitroso/efeitos adversos , Succinilcolina/efeitos adversos , Adolescente , Colonoscopia , Humanos , Linfangiectasia Intestinal/diagnóstico , Linfangiectasia Intestinal/patologia , Linfangiectasia Intestinal/cirurgia , Masculino , Hipertermia Maligna/patologia , Hipertermia Maligna/terapia , Músculos/patologia , Pacientes AmbulatoriaisAssuntos
Radiografia Pulmonar de Massa/métodos , Sarcoidose/prevenção & controle , Adolescente , Adulto , Fatores Etários , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Sarcoidose/epidemiologia , Sarcoidose/patologia , Fatores Sexuais , SuíçaAssuntos
Bronquite/epidemiologia , Adulto , Idoso , Poluição do Ar , Doença Crônica , Surtos de Doenças , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fumar/complicações , SuíçaRESUMO
Some in vitro functions of purified T and B cells from PPD- and Candida albicans-negative sarcoidosis patients were analyzed. It appears that in those patients there is: 1) A decrease in the absolute number of T lymphocytes and an increase in the number of B cells; 2) a rather normal response of T lymphocytes to PHA and Con A; 3) a rather normal capacity of T and B cells to produce MIF in vitro; and 4) an ability of T cells from sarcoid patients (but not B cells) to produce LMF. These results suggest that the frequent deficit in cell-mediated immunity observed in sarcoidosis seems to correlate with a quantitative deficit in T cells. The cause of this T-cell deficit is unknown.