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1.
Microb Cell Fact ; 16(1): 16, 2017 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-28137256

RESUMO

BACKGROUND: Through functional screening of a fosmid library, generated from a phytopathogen-suppressive soil metagenome, the novel antifungal chitinase-named Chi18H8 and belonging to family 18 glycosyl hydrolases-was previously discovered. The initial extremely low yield of Chi18H8 recombinant production and purification from Escherichia coli cells (21 µg/g cell) limited its characterization, thus preventing further investigation on its biotechnological potential. RESULTS: We report on how we succeeded in producing hundreds of milligrams of pure and biologically active Chi18H8 by developing and scaling up to a high-yielding, 30 L bioreactor process, based on a novel method of mild solubilization of E. coli inclusion bodies in lactic acid aqueous solution, coupled with a single step purification by hydrophobic interaction chromatography. Chi18H8 was characterized as a Ca2+-dependent mesophilic chitobiosidase, active on chitin substrates at acidic pHs and possessing interesting features, such as solvent tolerance, long-term stability in acidic environment and antifungal activity against the phytopathogens Fusarium graminearum and Rhizoctonia solani. Additionally, Chi18H8 was found to operate according to a non-processive endomode of action on a water-soluble chitin-like substrate. CONCLUSIONS: Expression screening of a metagenomic library may allow access to the functional diversity of uncultivable microbiota and to the discovery of novel enzymes useful for biotechnological applications. A persisting bottleneck, however, is the lack of methods for large scale production of metagenome-sourced enzymes from genes of unknown origin in the commonly used microbial hosts. To our knowledge, this is the first report on a novel metagenome-sourced enzyme produced in hundreds-of-milligram amount by recovering the protein in the biologically active form from recombinant E. coli inclusion bodies.


Assuntos
Antifúngicos/farmacologia , Quitinases/metabolismo , Quitinases/farmacologia , Escherichia coli/genética , Hexosaminidases/metabolismo , Hexosaminidases/farmacologia , Microbiologia do Solo , Antifúngicos/isolamento & purificação , Antifúngicos/metabolismo , Reatores Biológicos , Quitina/metabolismo , Quitinases/genética , Quitinases/isolamento & purificação , Clonagem Molecular , Escherichia coli/metabolismo , Fusarium/efeitos dos fármacos , Biblioteca Gênica , Hexosaminidases/genética , Hexosaminidases/isolamento & purificação , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Corpos de Inclusão/enzimologia , Ácido Láctico/metabolismo , Metagenoma , Metagenômica/métodos , Filogenia , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/farmacologia , Rhizoctonia/efeitos dos fármacos
2.
Appl Environ Microbiol ; 80(17): 5170-7, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24814791

RESUMO

Bifidobacteria are Gram-positive inhabitants of the human gastrointestinal tract that have evolved close interaction with their host and especially with the host's immune system. The molecular mechanisms underlying such interactions, however, are largely unidentified. In this study, we investigated the immunomodulatory potential of Bifidobacterium bifidum MIMBb75, a bacterium of human intestinal origin commercially used as a probiotic. Particularly, we focused our attention on TgaA, a protein expressed on the outer surface of MIMBb75's cells and homologous to other known bacterial immunoactive proteins. TgaA is a peptidoglycan lytic enzyme containing two active domains: lytic murein transglycosylase (LT) and cysteine- and histidine-dependent amidohydrolase/peptidase (CHAP). We ran immunological experiments stimulating dendritic cells (DCs) with the B. bifidum MIMBb75 and TgaA, with the result that both the bacterium and the protein activated DCs and triggered interleukin-2 (IL-2) production. In addition, we observed that the heterologous expression of TgaA in Bifidobacterium longum transferred to the bacterium the ability to induce IL-2. Subsequently, immunological experiments performed using two purified recombinant proteins corresponding to the single domains LT and CHAP demonstrated that the CHAP domain is the immune-reactive region of TgaA. Finally, we also showed that TgaA-dependent activation of DCs requires the protein CD14, marginally involves TRIF, and is independent of Toll-like receptor 4 (TLR4) and MyD88. In conclusion, our study suggests that the bacterial CHAP domain is a novel microbe-associated molecular pattern actively participating in the cross talk mechanisms between bifidobacteria and the host's immune system.


Assuntos
Amidoidrolases/imunologia , Bifidobacterium/enzimologia , Bifidobacterium/imunologia , Diferenciação Celular , Células Dendríticas/imunologia , Peptidoglicano/metabolismo , Amidoidrolases/metabolismo , Animais , Fatores de Transcrição Hélice-Alça-Hélice Básicos , Parede Celular/química , Células Cultivadas , Cisteína/metabolismo , Histidina/metabolismo , Proteínas de Membrana/imunologia , Proteínas de Membrana/metabolismo , Camundongos Endogâmicos C57BL , Peptidoglicano/análise
3.
Appl Microbiol Biotechnol ; 98(6): 2819-28, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24121932

RESUMO

Plant disease caused by fungal pathogens results in vast crop damage globally. Microbial communities of soil that is suppressive to fungal crop disease provide a source for the identification of novel enzymes functioning as bioshields against plant pathogens. In this study, we targeted chitin-degrading enzymes of the uncultured bacterial community through a functional metagenomics approach, using a fosmid library of a suppressive soil metagenome. We identified a novel bacterial chitinase, Chi18H8, with antifungal activity against several important crop pathogens. Sequence analyses show that the chi18H8 gene encodes a 425-amino acid protein of 46 kDa with an N-terminal signal peptide, a catalytic domain with the conserved active site F175DGIDIDWE183, and a chitinase insertion domain. Chi18H8 was expressed (pGEX-6P-3 vector) in Escherichia coli and purified. Enzyme characterization shows that Chi18H8 has a prevalent chitobiosidase activity with a maximum activity at 35 °C at pH lower than 6, suggesting a role as exochitinase on native chitin. To our knowledge, Chi18H8 is the first chitinase isolated from a metagenome library obtained in pure form and which has the potential to be used as a candidate agent for controlling fungal crop diseases. Furthermore, Chi18H8 may also answer to the demand for novel chitin-degrading enzymes for a broad range of other industrial processes and medical purposes.


Assuntos
Bactérias/enzimologia , Quitinases/metabolismo , Fungos/efeitos dos fármacos , Metagenômica , Microbiologia do Solo , Bactérias/genética , Quitina/metabolismo , Quitinases/química , Quitinases/genética , Clonagem Molecular , DNA Bacteriano/química , DNA Bacteriano/genética , Estabilidade Enzimática , Escherichia coli/enzimologia , Escherichia coli/genética , Expressão Gênica , Biblioteca Gênica , Concentração de Íons de Hidrogênio , Dados de Sequência Molecular , Peso Molecular , Estrutura Terciária de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Análise de Sequência de DNA , Temperatura
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