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1.
Radiobiologiia ; 32(4): 560-5, 1992.
Artigo em Russo | MEDLINE | ID: mdl-1410294

RESUMO

The radiosensitivity of mouse myeloma and E. coli cells in the presence of Mg2+ and UO2(2+) ions has been investigated. It has been shown that Mg2+ ions (10(-4) M) do not influence the viability of E. coli and mouse myeloma cells. The presence of Mg2+ ions during irradiation reduces the survival rate of E. coli cells, but the addition of Mg2+ ions after irradiation does not influence the radiosensitivity of E. coli cells. Comparison of the results on the influence of Mg2+ ions upon cells and bilayer lipid membranes (BLM) permits us to suppose that Mg2+ ions increase the positive charge of the membranes thus promoting the increase in the number of short-lived radiolysis products which impair membranes and increase cell radiosensitivity. UO2(2+) ions (10(-4) M) increase the radioresistance of E. coli cells which can be associated with the increase in the lateral membrane viscosity, as it was shown in the studies on BLM.


Assuntos
Membrana Celular/efeitos da radiação , Tolerância a Radiação , Compostos de Urânio , Animais , Membrana Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Relação Dose-Resposta à Radiação , Escherichia coli/efeitos dos fármacos , Escherichia coli/efeitos da radiação , Bicamadas Lipídicas/efeitos da radiação , Magnésio/farmacologia , Lipídeos de Membrana/efeitos da radiação , Camundongos , Mieloma Múltiplo , Células Tumorais Cultivadas/efeitos dos fármacos , Células Tumorais Cultivadas/efeitos da radiação , Urânio/farmacologia
2.
Radiobiologiia ; 27(4): 435-40, 1987.
Artigo em Russo | MEDLINE | ID: mdl-3628723

RESUMO

As was shown by the pulse radiolysis method the simultaneous presence of naphthoquinone and human serum albumin molecules in an aqueous solution leads to the adsorption of the former on the surface of the latter. It is suggested that in these conditions the protein tertiary structure changes. New conformation reduces the reactivity of albumin toward the hydrated electron.


Assuntos
Naftoquinonas/farmacologia , Protetores contra Radiação , Albumina Sérica/efeitos da radiação , Humanos , Cinética , Conformação Proteica , Soluções
3.
Radiobiologiia ; 26(2): 219-21, 1986.
Artigo em Russo | MEDLINE | ID: mdl-3517939

RESUMO

It was shown that the periodic radiation source frequency influences the rate of E. coli cell inactivation. The impulse duration from 2 microseconds to 40 ns does not influence the survival of E. coli. The analysis of the survival curves obtained permitted to assess the contribution of the direct and indirect effects of radiation to microorganism inactivation.


Assuntos
Escherichia coli/efeitos da radiação , Doses de Radiação , Escherichia coli/crescimento & desenvolvimento , Tolerância a Radiação
4.
Radiobiologiia ; 25(2): 169-73, 1985.
Artigo em Russo | MEDLINE | ID: mdl-3889989

RESUMO

It was proved that the method of pulse radiolysis can be used to study the processes rapidly occurring in a microorganism suspension. It was shown that optically active particles formed after irradiation in an E. coli cell were involved into the first-(pseudo-first)-order reaction the rate constant being approximately 30 s-1. The presence of oxygen did not influence the kinetics of disappearance of these particles.


Assuntos
Escherichia coli/efeitos da radiação , Radiólise de Impulso , Análise Espectral , Elétrons , Escherichia coli/metabolismo , Cinética
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