[Heterogeneity of cell population of lymphoma NK/Ly and leukemia L-1210 according to carbohydrate structure of cell surface: immunocytochemical analysis of lectin binding].

The heterogeneity of tumor cell populations according to binding of lectins from lentil (LcL), wheat germs (WGA), peanut (PNA) and concanavalin A was investigated on a model of murine Nemeth-Kellner lymphoma (NK/Ly) and leukemia L-1210. Bound lectins were detected by indirect immunochemical method using home obtained polyclonal antilectin antibodies and immunogold silver staining (IGSS) technique. Significant differences in binding of Con A were revealed between NK/Ly (67% Con A+) and L-1210 (7.2% Con A+) cells, while the differences in binding of other lectins with both types of tumor cells were not significant. A relatively high percentage of PNA+ cells was registered that can indicate a high degree of desialization of membrane glycoproteins.

[The biological activity of Pseudomonas solanacearum polysaccharide]. / Biologicheskaia aktivnost' lipopolisakharida Pseudomonas solanacearum.

Antitumour, antileukosis and antimetastatic effects of lipopolysaccharide (LPS) isolated from Pseudomonas solanacearum have been studied. It is established that LPS does not possess the antitumour effect on experimental animals with Lewis lung carcinoma, melanoma B-16 and sarcoma S-37 and vice versa, intensifies the tumour growth. The life time of animals with experimental leukoses lymphocytic leukemia P-388 and lymphoid leukemia L 1210 inconsiderably increases. At the same time LPS possesses the expressed antimetastatic effect that has manifested in the decrease of the volume (40 and 5 times) and of the amount (4-4.2 times) of metastases in mice with Lewis lung carcinoma and melanoma B-16, respectively. Study of the contribution of certain structural components of LPS molecule to the total biological activity has shown that O-specific polysaccharide and oligosaccharide of core take the expressed antimetastatic effect. Lipid A in the used dose weakly modified the development of Lewis lung carcinoma metastases.

[The effect of emotional-pain stress syndrome on the biochemical characteristics of the stress-realizing system and metastasis of Lewis lung carcinoma in mice]. / Vliianie émotsional'no-bolevogo stressa na biokhimicheskie kharakteristiki stressrealizuiushchikh sistem i metastazirovanie kartsinomy legkikh L'iuis u myshei.

The emotional-pain stress (EPS) in C57BL mice with Lewis carcinoma was studied for its influence on serotoninergic, noradrenergic, dopaminergic, glutamatergic, aspartatergic, glycinergic, taurinergic, GABA-ergic and cholinergic mediator mechanisms of the hypothalamus, the level of corticotropin, free and bound 11-corticosteroids, insulin, thyroxin and testosterone in blood plasma as well as on the content of catecholamines, their precursors and catabolites in urine. EPS contributed to a long-term activation of stress-realizing systems resulting in generalization of decompensation in them. The stimulating effect of EPS on the incidence and growth of metastases is established depending on the terms of its application.

[Changes in neurohumoral factors of various tissues in patients with breast cancer]. / Izmeneniia nekotorykh tkanevykh neirogumoral'nykh faktorov u bol'nykh rakom molochnoi zhelezy.

Such neurohumoral factors of antitumor resistance regulation as catecholamines, corticosteroids and cholinergic mechanisms were studied in tissues of breast cancer patients. Tissue adjacent to tumor revealed elevated concentrations of 11-hydroxycorticosteroids and adrenaline matched by an increased release of norepinephrine which greatly depended on weakened cholinergic influences. These disturbances were pronounced in stages I and II tumors and, particularly, in infiltrating scirrhous cancer. Disbalance in neurohumoral factor distribution between normal and altered tissues may boost tumor recurrence and metastatic spread.

[Neurohormonal changes during the growth of metastatic Lewis carcinoma in C57Bl mice]. / Nerogormonal'nye izmeneniia v protsesse rosta metastaziruiushchei kartsinomy L'iuis u myshei C57Black.

Functions of adrenal gland cortex, mediator system of brain and of sympathoadrenal system, including hormone formation in medullar layer of adrenal glands, were studied in mice of C57BL strain with growing metastatic Lewis carcinoma. The neurohormonal activity was distinctly inhibited under conditions of stress within the period of adaptation to the tumor development (I-14 days after the carcinoma inoculation) as well as in development of metastases (21 day of the tumor growth). Excessive stimulation of the systems studied as well as their exhaustion, might cause a distinct augmenting effect on the tumor growth and development of metastases.

[The pituitary-adrenal system in cancer of the esophagus and stomach]. / Gipofizatno-nadpochechnikovaia sistema pri rake pishchevoda i heludka.

Excretion of corticosteroids, levels of 11-OCS in blood plasma, tests with dexasone and clinical evaluation of the pituitary-adrenal system function were studied in 354 patients with carcinomas of the esophagus and proximal stomach division. Some degree of hypocorticism with a decrease in the 11-OCS level in blood plasma and lowered excretion of corticosteroids in the urine were found in 71% of patients. These variations are accompanied by lowered hypothalamus sensitivity to dexasone. Similar disturbances though less pronounced are determined in the precancerous states and in the remote periods after operation.

[Metastasis and stress reactions in the surgical resection of tumors]. / Metastazirovanie i stressornye reakstii pri khirurgicheskom udalenii opukholi.

Complex investigations of brain mediator systems, catecholamine metabolism, II-oxycorticosteroid level in blood plasma, histochemical study of hormonogenesis in adrenal medulla and adrenal cortex in C57N1 mice with metastatic Lewis (3LL) carcinoma showed that metastatic stimulation initiated by the removal of a tumour caused an intensive prolonged stress reaction which differed from the reaction to a surgical trauma without removing a tumour. It is supposed that neuroendocrinal stress reactions could to a considerable extent determine this metastatic stimulation.

[Correction of hypophyseal-adrenal system function in esophageal and stomach cancer patients undergoing combined and surgical treatments]. / Korrektsiia funktsii gipofizarno-nadpochechnikovoi sistemy u bol'nykh rakom pishchevoda i zheludka pri kombinirovannom i khirurgicheskom lechenii.

The paper presents the data on marked dysfunction of hypothalamo-pituitary-adrenal system in patients with esophageal and gastric cancer. Patients revealed lowered levels of 11-hydroxycorticosteroids in blood plasma and 17-corticosteroids and 17-glucocorticosteroids in diurnal urine. It was matched by an elevated threshold of hypothalamic sensitivity to inhibition by glucocorticosteroids. Treatment for these disturbances which brought the function of this system to normal had a beneficial effect on the secretion and excretion of corticosteroids in the course of combined therapy and surgery and was followed by a significant decrease in hemodynamic complication incidence. The favorable clinical effect was in correlation with the decrease in hypothalamic resistance to the inhibitory effect of glucocorticoids.

[Effect of hypothalamic electrostimulation on the 11-hydroxy-corticosteroid content in the blood and on the immunological indices in rabbits with Brown-Pearce carcinoma]. / Vliianie élektrostimuliatsii gipotalamusa na soderzhanie 11-oksikortikosteroidov v krovi i immunologicheskie pokazateli u krolikov s kartsinomoi Brauna-Pirs.

In animals with Brown--Pearce carcinoma electric stimulation of the hypothalamus makes it possible to model an increased corticosterone level in blood at early stages of the tumor growth, which conditions more active development of antitumor reactions and consequently an enhanced tumor resorption. The increased level of free corticosterone seems to be essential for "triggering" and an active mode of proceeding of antitumor immune reactions.