[Differential bioinformational model for diagnostics of inflammatory and tumor diseases of the parotid salivary gland]. / Differentsial'naya bioinformatsionnaya model' diagnostiki vospalitel'nykh i opukholevykh zabolevanii okoloushnoi slyunnoi zhelezy.

THE AIM: Of the work was to develop a diagnostic algorithm for the differentiation of chronic inflammatory, benign and malignant processes in the parotid salivary gland (PSG) by the ratio of pro- and anti-inflammatory cytokines in the oral fluid. MATERIALS AND METHODS: The epidemiological group of patients with cancer of the parotid salivary gland included 140 people from the oncological register of the Rostov region with the date of diagnosis, from 1969 to 2020. The clinical part of the work was performed on 70 patients of both sexes aged 50 to 80 years: 15 patients with chronic nonspecific parenchymal sialadenitis of the PSG (ICD K11.2) (group 1), 19 patients with pleomorphic adenoma of the PSG (ICD D11.0) (2 group), 20 patients with cancer of the PSG (ICD C07) (group 3) and 16 healthy individuals without pathology of the oral cavity (control group). The concentration of interleukin-6 (IL-6) and interleukin-10 (IL-10) was determined in the oral fluid by enzyme immunoassay. RESULTS: It was found that in 58.5% of cases at the initial examination of patients with PSG cancer referred to a tertiary care hospital an erroneous opinion was formed about the inflammatory origin of the process. In inflammatory and tumor lesions of the PSG multidirectional differences are noted in the ratio between the concentrations of pro- and anti-inflammatory mediators in the oral fluid. In chronic sialadenitis of PSG in the oral fluid a moderate increase in the levels of IL-6 and IL-10 occurs, in the presence of adenoma of PSG, the concentration of IL-6 does not change while IL-10 increases threefold, and there is a sharp and unidirectional increase in the concentration of cytokines of the opposite groups in case of a malignant lesion of PSG. CONCLUSION: Comparison of the concentration of IL-6 and IL-10 in saliva and their ratio defined by the developed discriminant models helps to make an individual diagnostic decision in a specific clinical situation.

[Dynamics of bone metabolism markers in oral fluid after surgical treatment of chronic periodontal disease with various osteoplastic materials]. / Dinamika kostnogo metabolizma posle khirurgicheskogo lecheniia khronicheskogo generalizovannogo parodontita s podsadkoi razlichnykh kostno-plasticheskikh materialov po pokazateliam rotovoi zhidkosti.

The aim of the work was to assess the balance between osteosynthetic and osteodestructive processes in the oral cavity in patients with chronic generalized periodontitis (CGP) of moderate and severe severity in the dynamics of a remote period of patchwork using various bone-plastic materials (BPM) based on xenogeneic hydroxyapatite (HA), ß-tricalcium phosphate (TCP). 123 patients with CGP were examined. Depending on the type of BPM, two groups were distinguished by composition in the course of patchwork operations. Patients of group 1 (n=61) received BPM on the basis of xenogeneic HA, 2 groups (n=62) on the basis of ß-TCP. Initially and after surgery at 8 and 12 months in the oral fluid, the concentration of osteoprotegerin (OPG), the ligand of the soluble activator of the kappa B nucleation factor (sRANKL), the bone isoenzyme of alkaline phosphatase (BAP), was determined by an immunoenzyme method. As a result of the study, it was found that in patients with moderate-bodied CGP after surgical treatment, restriction of osteoclast activity with a decrease in sRANKL due to an increase in OPG in the oral fluid, an increase in osteoblast activity with BAP secretion is most pronounced when using ß-TCP-based BPM at 8 months. After surgery with an average severity of the disease. Less pronounced changes in bone metabolism in the oral cavity are detected with the use of BPM on the basis of HA. In severe CGP, changes in bone homeostasis in the oral cavity after scrappy operations with BPM insertion based on TCP or HA are not expressed and are observed only after 12 months. Thus, the most effective changes in bone metabolism develop with the use of TCP as BPM.

[Diagnostic value of serum osteomarkers in moderate and severe periodontal disease]. / Diagnosticheskaia informativnost' opredeleniia osteomarkerov v syvorotke krovi pri khronicheskom generalizovannom parodontite srednei i tiazheloi stepeni.

The purpose of the study was to establish the diagnostic informative value of the concentration of osteomarkers in the serum to assess the severity of the osteodestructive component in chronic generalized periodontitis (HGP) prior to surgery and the prognosis of the effectiveness of surgical treatment of the disease. 187 patients with moderate and severe HGP who underwent bone and plastic insertion during the patch operations were examined. In the blood serum, the concentration of osteoprotegerin, ligand of the soluble activator of the kappa B nucleation factor (sRANKL) was initially determined and at 8 and 12 months after the operation, bone isoenzyme of alkaline phosphatase, osteocalcin, C-terminal telopeptides of degradation of mature type 1 collagen (ß-CrossLaps). It was found that in assessing the severity of osteodestructive processes in the periodontium in HGP, the serum concentration of sRANKL and ß-CrossLaps is informative. When the sRANKL content in the blood serum exceeds 0,48 pmol/l, and ß-CrossLaps exceeds 0,795 ng/ml, it is possible to judge the high activity of osteoclasts, which is accompanied by a pronounced risk of irreversibility of the osteodestructive component and the lack of effectiveness of surgical treatment.

[Diagnostic and prognostic significance of the hypoxia-dependent transcriptional mediator for the development of a carious lesion]. / Diagnosticheskaia i prognosticheskaia znachimost' gipoksiiazavisimogo transkriptsionnogo mediatora dlia razvitiia karioznogo porazheniia.

OBJECTIVE: To study diagnostic informational content of change of concentration a hypoxia-inducible factor (HIF-1α) in gingival liquid of patients for assessment of risk of development of carious process. MATERIAL AND METHODS: 75 patients with the diagnosis dentine caries entered the research: group 1 (n=30) involved patients with primary caries while group 2 (n=45) consisted of patients with caries recurrence in 12 months after treatment. RESULTS: In all patients HIF-1α was determined in gingival liquid before treatment by the IFA method. Logistic regression and the ROC analysis were used for the statistical analysis. In group 2 the level of HIF-1α was two-fold higher than in group 1 (p<0.001) and 82% higher than in healthy individuals (p<0.001). The increased initial HIF-1α level of 98 pg/ml was associated with high risk of caries recurrence in 12 months after treatment with diagnostic sensitivity of 88.4% and diagnostic specificity of 78.1% with chances of development increased by 4 times (p<0.0001). The change of HIF-1α in gingival liquid from 80 pg/ml to 170 pg/ml increased the risk of caries recurrence 12 months from 13% up to 94%. CONCLUSION: The initial content in gingival liquid a hypoxia-inducible factor-1α higher than 98 pg/ml allows triage of patients with high risk of caries recurrence requiring dental monitoring for personification of preventive measures.