[New phosphonoformic acid derivatives of 3'-azido-3'-deoxythymidine]. / Novye proizvodnye 3'-azido-3'-dezoksitimidina i fosfonomurav'inoi kisloty.

New 5'-alkyl ethoxy- and aminocarbonylphosphonates of 3'-azido-3'-deoxythymidine (AZT) were synthesized, and their antiviral properties in HIV-1-infected cell cultures and stability to chemical hydrolysis were studied. The AZT 5'-aminocarbonylphosphonates were shown to be significantly more stable in phosphate buffer (pH 7.2) than the corresponding ethoxycarbonylphosphonates. The therapeutic (selectivity) index of some of the compounds exceeded that of the parent AZT due to their higher antiviral activity. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 3; see also http://www.maik.ru.

[Immunogenetic properties of peptides mimicking a human immunodeficiency virus gp41 (HIV-1) epitope recognized by virus-neutralizing antibody 2F5]. / Immunogennye svoistva peptidov-imitatorov épitopa belka gp41 virusa immunodefitsita cheloveka (VICh-1), uznavaemogo neitralizuiushchim antitelom 2F5.

Phage display was used to obtain peptides mimicking a HIV-1 gp41 conserved epitope recognized by virus-neutralizing monoclonal antibodies (MCA) 2F5. Rabbits and mice were immunized with the peptides exposed on the surface of filamentous bacteriophages. Antibodies to gp41 were detected in the sera of immunized animals. The virus-neutralizing activity of the sera was examined.

[Immunostimulating effect of plant triterpenes and their derivatives]. / Immunostimuliruiushchaia aktivnost' triterpenov rastitel'nogo proiskhozhdeniia i ikh proizvodnykh.

The immunostimulating activity of triterpenes of vegetable origin and their derivatives was studied. The study revealed that glycyrrhizic and betulonic acids, as well as derivatives of ursolic and glycyrrhetinic acids, enhanced the level of specific antibodies to bovine serum albumin in mice.

[Content of progesterone, glucocorticoid and glycyrrhizic acid receptors in normal and tumoral human breast tissue]. / Soderzhanie retseptorov progesterona, gliukokortikoidov i glitsirrizinovoi kisloty v opukholevoi i normal'noi tkani molochnoi zhelezy cheloveka.

Progesterone and glucocorticoid receptor levels were assayed in breast tumor. The progesterone levels in tumor were 1.2-2.5 times those in normal tissue sampled from the same patients. The binding of glycyrrhizic acid (GA) to cytoplasmic proteins (GA "receptor" levels) was studied using procedures for glucocorticoid receptor assay. The "receptor" concentrations in tumor tissue were 1.5-5 times that in normal one. The "receptors" were detected only in 5.9% of normal breast tissue samples. The Kd of GA-protein complex was ca.7 x 10(-8) M. GA seems to offer significant advantage as a drug-carrier involved in therapy of glucocorticoid-dependent breast cancer.

[The characteristics of the long-term continuous cultivation of a cell line chronically infected with the human immunodeficiency virus type-1]. / Osobennosti dlitel'nogo nepreryvnogo kul'tivirovaniia linii kletok, khronicheski infitsirovannoi virusom immunodefitsita cheloveka 1 tipa.

A chronically HIV-1-infected culture of continuous human monocytes producing infectious virus during long-term continuous cultivation (over 200 passages) without the addition of noninfected cells was generated. HIV-1 produced by this culture had typical morphology and complete set of virus-specific proteins. This cell line is proposed for use as an additional model for the evaluation of antiviral effects of various drugs.