RESUMO
We studied the effect of 20 standard L-amino acids on proliferation of the nervous, cardiovascular, urogenital, digestive, and immune system tissues from young and old animals in organotypic cultures. The effect of amino acids on tissue culture proliferation depended on their origin and animal age.
Assuntos
Aminoácidos/fisiologia , Envelhecimento , Animais , Encéfalo/citologia , Proliferação de Células , Masculino , Miocárdio/citologia , Especificidade de Órgãos , Ratos Wistar , Regeneração , Baço/citologia , Técnicas de Cultura de TecidosRESUMO
The review considers literature data and own results of research of cytokines functions and their effects on hemostatic system and life span. The data of age-related changes in the hemostatic system is presented in this article. A big part of the review is devoted to the action of regulatory peptides (RP) on various body systems. It is established that the RP can normalize the expression of cytokine genes in humans and animals with stress and pathological conditions. Effect of RP on the cytokines has geroprotective action, which is based on anti-oxidant, anti-inflammatory action, stimulation of cell proliferation and differentiation, as well as a normalizing effect on the immune system and hemostasis.
Assuntos
Citocinas/metabolismo , Hemostasia , Fatores Etários , Animais , Citocinas/genética , Regulação da Expressão Gênica , Humanos , Estresse FisiológicoRESUMO
We studied Molecular mechanisms of the retinoprotective effect of short peptides AEDG and KE. The peptides stimulate differentiation of neurons and retinal pigment epithelium cells and therefore can be considered as potential retinoprotective preparations for the treatment of age-related degenerative changes in the retina.
Assuntos
Diferenciação Celular/efeitos dos fármacos , Neurônios/citologia , Peptídeos/farmacologia , Retina/metabolismo , Epitélio Pigmentado da Retina/citologia , Animais , Células Cultivadas , Embrião de Galinha , Galinhas , Retina/citologiaRESUMO
Analysis of the main parameters of molecular mechanics (number of hydrogen bonds, hydrophobic and electrostatic interactions, DNA-peptide complex minimization energy) provided the data to validate the previously proposed qualitative models of peptide-DNA interactions and to evaluate their quantitative characteristics. Based on these estimations, a three-dimensional model of Lys-Glu and Ala-Glu-Asp-Gly peptide interactions with DNA sites (GCAG and ATTTC) located in the promoter zones of genes encoding CD5, IL-2, MMP2, and Tram1 signal molecules.
Assuntos
DNA/química , Dipeptídeos/química , Metaloproteinase 2 da Matriz/genética , Glicoproteínas de Membrana/genética , Proteínas de Membrana Transportadoras/genética , Oligopeptídeos/química , Regiões Promotoras Genéticas , Sequência de Bases , Sítios de Ligação , Antígenos CD5/genética , Peptídeos Penetradores de Células/metabolismo , DNA/metabolismo , Proteínas de Ligação a DNA/análise , Dipeptídeos/metabolismo , Interleucina-2/genética , Dados de Sequência Molecular , Oligopeptídeos/metabolismo , Fragmentos de Peptídeos , Eletricidade EstáticaRESUMO
We developed a method of culturing and phenotyping of a monolayer of cells of the retinal tissue, thymus and spleen on the basis of organotypic culture. All characteristic types of neurons and fibroblasts were found in their microenvironment in the retinal cell monolayer. Lymphocytes, macrophages, and fibroblasts were verified in the monolayer of thymus and spleen cells. Histological staining, immunocytochemistry, and electron microscopy demonstrated the possibility of assessing the differentiation degree and functional activity of the cell monolayer. The developed technique preserves cell-cell interactions and a variety of cell types characteristic of the examined organ in the monolayer. This opens up new prospects for its application in basic research and in screening of different physiologically active substances.
