ARG16GLY POLYMORPHISM IN THE ß2-ADRENOCEPTOR GENE IN PATIENTS WITH BRONCHIAL ASTHMA.

OBJECTIVE: The aim: The objective of the study was to analyze the frequency of Arg16Gly polymorphism in the ß2 -adrenoceptor (ß2 -АR) gene in patients with bronchial asthma (BA) and to assess the association of the polymorphism with BA risk. PATIENTS AND METHODS: Materials and methods: We examined 553 BA patients and 95 apparently healthy individuals. Arg16Gly polymorphism in the ß2 -АR gene (rs1042713) was determined using polymerase chain reaction-restriction fragment length polymorphism analysis. Statistical analysis of obtained results was performed using SPSS-17 program. RESULTS: Results: It was established that distribution of Arg/Arg, Arg/Gly, and Gly/Gly genotypes for Arg16Gly polymorphism in the ß2 -АR gene was 44.2%, 40.0%, 15.8% in the control group vs. 31.3%; 45.7% and 23.0 among BA patients, respectively (χ2 = 6.59; р = 0.037). No significant difference was observed with regards to the distribution of genotypes for Arg16Gly polymorphism in the ß2 -АR gene in men and women controls (χ2 = 4.05; р = 0.13) and BA patients (χ2 = 4.34; р = 0.11). BA risk was 1.74 times higher in the minor allele carriers (Arg/Gly + Gly/Gly genotypes) for Arg16Gly polymorphism in the ß2 -АR gene. CONCLUSION: Conclusions: Analysis of Arg16Gly polymorphic variants in the ß2-AR gene showed a statistically significant difference in the distribution of Arg/Arg, Arg/Gly, and Gly/Gly genotypes in patients with BA and apparently healthy individuals due to the higher frequency of Arg/Arg genotype in controls and higher frequency of Gly/Gly genotype in patients with asthma. No difference with regard to gender was found in the distribution of genotypes.

The impact of systemic inflammation on anemia in patients with chronic obstructive pulmonary disease.

OBJECTIVE: The aim of our work was to study the indicators of systemic inflammation in patients with chronic obstructive pulmonary disease (COPD) with anemia of chronic disease (AHD). PATIENTS AND METHODS: Materials and methods: The study included 144 COPD patients (1 group) without anemia (hemoglobin> 120 g/l for women and> 130 g/l for men), and 33 patients (2 group) with COPD and ACD (hemoglobin <120 g/l for women and <130 g/l for men, soluble transferrin receptors (sTFR) - 8.7 - 28.1 nmol/l). The control group included 62 practically healthy people. All patients were evaluated for the content of ferritin, C-reactive protein (C-RP) and hepcidin. Statistical processing of the results was performed by using the SPSS-21 program. RESULTS: Results: Patients with COPD and ACD have a significantly higher ferritin level (p <0.001) compared to COPD patients without anemia and patients in the control group. The content of C-RP and hepcidin in patients with COPD and ACD is also significantly (p <0.001) higher compared to patients without anemia and patients in the control group. CONCLUSION: Conclusions: Determination of the content of ferritin, C-RP and hepcidin in patients with COPD may allow adequate treatment for this group of patients.

Association of metabolic syndrome components with the genotypes of the С825Т polymorphism in the g protein ß3-subunit gene (GNB3).

OBJECTIVE: Introduction: All components of the metabolic syndrome (MS) are the risk factors for the cardiovascular diseases, and their combination a great deal accelerates and complicates development of the diseases. Phenotypic expression of MS depends on the interaction of genetic and environmental factors. The aim: The aim is to study the association of metabolic syndrome components with the genotypes of the C825T polymorphism in the GNB3 gene, which allows predicting the risks and determining individual lifestyle and treatment program for the future. PATIENTS AND METHODS: Materials and methods: The patients were analyzed for anthropomorphic data and abdominal obesity. Presence of MS criteria was assessed in the patients in accordance with the consensus of International Diabetes Federation (2009). Hypertension diagnosis is based on the recommendations of adapted Clinical Practice Guidelines "Arterial Hypertension" (2012). The study of the С825Т polymorphism in the G protein ß3-subunit gene was conducted at molecular-genetic research laboratory of Sumy State University with subsequent analysis of restriction fragment length polymorphism. The data were statistically processed using SPSS Statistics 21.0 program on PC. RESULTS: Results: T-allele carriers had 1.6 times higher risk of obesity than C-allele homozygotes ( р=0.034). Т825Т genotype carriers were 2.7 times higher risk of hypertension compared the carriers of С825С genotype of the С825Т polymorphism of the GNB3 gene ( р = 0.002). The risk of increased LDL cholesterol level in the minor allele carriers is 3.9 times higher than that in the major allele carriers. ( р = 0.002). CONCLUSION: Conclusions: The results of our study concerning the association of the minor allele and T825 + C825T genotypes with the risk of components of the MS.