Assuntos
Antígenos/análise , Autoantígenos/análise , Pele/imunologia , Sistema ABO de Grupos Sanguíneos , Animais , Membrana Basal/imunologia , Bovinos , Núcleo Celular/imunologia , Colágeno/imunologia , Epiderme , Fibronectinas/imunologia , Cobaias , Antígenos de Histocompatibilidade/análise , Humanos , Hialina/imunologia , Queratinas/imunologia , Ratos , Reticulina/imunologia , Pele/ultraestruturaRESUMO
Mouse skin was scalded in vitro by the wet (immersion) method and dry (metal stamp) method. For in vivo scalding only the dry method was used. The content of dry mass of skin was investigated. In extracts of scalded skin the content of soluble components: protein, collagen, albumin and IgG was determined. The analyses and statistic estimation revealed that with respect to the observed effects the in vivo scalding of skin is similar to the ion vitro dry scalding but different from the in vitro wet method. Moreover, it is suggested that protein substances which are products of thermic denaturation of skin can penetrate from scalded skin into the surrounding tissues.
Assuntos
Queimaduras/metabolismo , Pele/lesões , Albuminas/metabolismo , Animais , Colágeno/metabolismo , Globulinas/metabolismo , Temperatura Alta , Imunoglobulina G/metabolismo , Técnicas In Vitro , Camundongos , Pele/metabolismoRESUMO
Human skin and the skin of some laboratory animals was minced, scalded and homogenized. The preparation from scalded human skin (the "crude burn toxin") revealed to be highly toxic to mice after i.v. administration. It was found by immunodiffusion and immunoelectrophoresis, that the urea and buthanol extracts of the crude "toxin" contain tissue antigens which were absent in a control preparation from a native (non scalded) skin.
