RESUMO
Hemagglutination-inhibiting serum antibodies to BK virus (BKV were detected in patients with different forms of cancer and in blood donors (positivity percentages: 67.04 and 57.78, respectively). No such antibodies were found in a group of children 1 to 14 years of age. The data point to the presence of a latent human infection with BKV in the population of Romania.
Assuntos
Anticorpos Antivirais/análise , Vírus BK/imunologia , Neoplasias Encefálicas/imunologia , Neoplasias/imunologia , Doenças do Sistema Nervoso/imunologia , Polyomavirus/imunologia , Adolescente , Adulto , Fatores Etários , Idoso , Doadores de Sangue , Criança , Pré-Escolar , Testes de Inibição da Hemaglutinação , Humanos , Lactente , Pessoa de Meia-Idade , RomêniaRESUMO
Hemagglutination-inhibiting antibodies to polyoma virus could be detected in sera from apparently healthy subjects, patients with nonmalignant respiratory diseases and patients with different forms of cancer. The positivity percentages (12.97, 12.92 and 16.36, respectively) and the titers recorded were lower than in the case of BK virus. The results obtained suggest a slight antigenic relationship between polyoma and BK virus.
Assuntos
Anticorpos Antivirais/análise , Neoplasias/imunologia , Polyomavirus/imunologia , Doenças Respiratórias/imunologia , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Testes de Inibição da Hemaglutinação , Humanos , Lactente , Pessoa de Meia-Idade , RomêniaRESUMO
With a view to detecting infections and carriage of human respiratory viruses in wild birds, 349 serum samples collected from 21 bird species -- sedentary ones and birds with large or limited migration areas -- were investigated. The following antigens were used: influenza virus A/Hong Kong 1/68 (H3N2)), A2 England 42/73 (H3N2), A2 Victoria 3/75 (H3N2), A/New Jersey 8/76 (HswN1), B/Hong Kong 5/72; parainfluenza virus type I Sendai and type II, coronavirus OC/43. An elevated incidence of antibodies to A2 Victoria 3/75 (H3N2) and parainfluenza type I virus was detected in herons (Ardea cinerea, Nyctycorax myctycorax). The high incidence of antibodies to B/Hong Kong 5/72 (30.7% of the samples exhibited significant titers) found in the crow (Corvus corone sardonius) is ascribed to the fact that this bird is carnivorus, feeding on corpses of mammals.
Assuntos
Aves , Reservatórios de Doenças , Infecções Respiratórias/transmissão , Viroses/transmissão , Animais , Europa Oriental , Humanos , Influenza Humana/transmissão , Infecções por Paramyxoviridae/transmissão , RomêniaRESUMO
The authors establish the optimal conditions (protein concentration, temperature) for the coupling of purified mycoplasma antigen to sheep red blood cells, in order to obtain an antigenic complex stable for at least 1 year. This comples allows specific determination by passive hemagglutination--a simple and rapid technique--of anti-mycoplasma antibodies in the sera of men or animals suspected of mycoplasma infections. Under the stated experimental conditions, antibody titers equal to or higher than 1/128 indicate an infection with M. pneumoniae.
Assuntos
Antígenos de Bactérias , Testes de Hemaglutinação , Infecções por Mycoplasma/diagnóstico , Animais , Anticorpos Antibacterianos/análise , Humanos , MycoplasmaAssuntos
Hepatite Viral Humana/epidemiologia , Adolescente , Adulto , Fatores Etários , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Romênia , População Rural , Estações do Ano , Fatores Sexuais , População UrbanaRESUMO
Complement fixation (CF) and passive hemagglutination (PHA) tests (the latter with a M. pneumoniae antigen coupled by glutaraldehyde onto red blood cells) were performed in 263 patients with various infectious diseases (mostly in the 1st and 2nd week after onset) and non-infectious ones. CF reaction proved to be inappropriate for the early etiological diagnosis of mycoplasma infections, since the high titers were distributed undifferentially among the various patient groups and many sera (38%) showed anticomplementary activity. A PHA titer of at least 1/128 (preferably of 1/512) points to the presence of a M. pneumoniae infection, especially if clinical, radiological and laboratory data suggest a nonbacterial or mixed pneumonia. The diagnosis is often early enough to orientate the etiological therapy towards macrolides and tetracyclines. The PHA reaction recommended is specific, sensitive, reproducible and easy to perform.
Assuntos
Testes de Fixação de Complemento , Testes de Hemaglutinação , Infecções por Mycoplasma/diagnóstico , HumanosRESUMO
Serological investigations were performed in order to establish the antigenic relationships between three Chlamydia strains: T44(subgroup A), T13 and Q18 (subgroup B). By using monospecific mouse immune sera a certain degree of antigenical relationships was made evident and the strains could be typed by microagglutination and microimmlnofluorescence reactions. Similar results were obtained by seroneutralization with hyperimmune sera prepared in the rooster. Complement fixation proved to be a group-specific reaction unable to reveal significant differences between the strains studied, not even with monospecific mouse sera. The antigenic relationships established between strains T44 (trachoma) and T13 (psittacosis) suggest the existence features common to subgroup A and B Chlamudia strains. The importance of typing Chlamudia strains with a view to specific prophylaxis is emphasized.
Assuntos
Antígenos de Bactérias , Chlamydia/imunologia , Animais , Antígenos de Bactérias/análise , Aves , Chlamydia/classificação , Humanos , Mamíferos , Camundongos , CoelhosRESUMO
In order to evaluate the efficiency of passive hemagglutination (PHA) and complement fixation (CF) tests for the diagnosis of M. pneumoniae infections, 144 serum samples--out of which 81 from patients with respiratory and cutaneous infections and 63 from subjects with other diseases (controls)--were comparatively investigated by the two methods. The PHA test made evident a positivity index of 65% in the case of mixed pneumonias, as against 17% in controls, while the results obtained by CF were almost identical in the two groups investigated. The high specificity and sensitivity of the PHA reaction recommend this method for the diagnosis of acute M. pneumoniae infections.