[Effect of He-Ne laser radiation on the chemiluminescence of mouse spleen cells]. / Vliianie izlucheniia He-Ne lazera na khemiliuminestsentsiiu kletok selezenki myshi.

A study was made of He-Ne laser radiation (lambda = 632.8 nm) on spontaneous chemiluminescence of mouse splenic cells and that stimulated by addition of Candida albicans. Irradiation with low-intensity red light was shown to stimulate cell chemiluminescence and to intensify that stimulated by C. albicans within the dose range from 100 to 300 J/m2 with a maximum at about 200 J/m2.

Helium-neon laser-induced respiratory burst of phagocytic cells.

The effect of He-Ne laser radiation (lambda = 632.8 nm, I = 6.8 W/m2, irradiation time from 5 to 50 sec) on kinetics of spontaneous and Candida ablicans-stimulated chemiluminescence of mouse spleen cells was studied. It was found that laser radiation caused significant enhancement (180-250%) both of spontaneous chemiluminescence and Candida-induced chemiluminescence. The effective dose interval ranges from 100 to 300 J/m2, with a maximum at 200 J/m2. This finding shows that He-Ne laser irradiation can induce the respiratory burst (generation of reactive oxygen species having bactericidal activity) of phagocytic cells.

[Chronic tick-bone encephalitis virus infection in continuous human lymphocyte lines studied by molecular hybridization]. / Izuchenie khronicheskoi infektsii virusa kleschevogo éntsefalita v perevivaemykh liniiakh limfotsitov cheloveka metodom molekuliarnoi gibridizatsii.

Chronic infection with tick-borne encephalitis virus (Sophyin strain) has been established in continuous lines of human lymphocytes (of T- and B-origin). In 3 lines under study, the cultured virus at the level of the 15th passage had a titre of 7.22-8.02 lg LD50ml, the virus-specific antigen was determined in the cytoplasm of 80% of T-lymphocytes (line 1387). The method of RNA-DNA hybridization demonstrated the presence of virus-specific sequences in DNA preparations from infected cells in amounts not exceeding 1 copy of virus genome per 10-25 cells.

[Modeling chronic infection in a T-lymphocyte-Venezuelan equine encephalomyelitis virus system]. / Modelirovanie khronicheskoi infektsii v sisteme T-limfotsit--virus venesuél'skogo éntsefalomielita loshadei.

A model of chronic infection in the system of T-lymphocyte--VEE virus was made. Production of infectious VEE (up to 6.0 lg PFU/ml), tissue culture interferon levels (less than 10 units/ml), accumulation of virus-specific antigen in chronically infected continuous human T-lymphocytes (78%) were studied. The presence of virus-specific sequence in DNA from infected cells was studied by the method of molecular hybridization and found not to exceed one DNA replica of virus genome per three cells of chronically infected T-lymphocyte culture.