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Vet Res Commun ; 48(5): 3423-3427, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39120674

RESUMO

The presence of infective larvae (L3) of gastrointestinal nematode (GIN) parasites in pastures directly contributes to the constant recurrence of infections in ruminant herds. This study aimed to evaluate the nematophagous fungus Duddingtonia flagrans (AC001) (proteolytic crude extract and/or conidia) in the in vitro control of GIN L3 in coprocultures. To produce the proteolytic crude extract, a suspension (107 conidia/mL) of D. flagrans was inoculated into a liquid medium. After 6 days, the medium was filtered, centrifuged, and its proteolytic activity was measured. For the experimental assay, fecal samples were collected directly from the rectal ampulla of naturally infected sheep, and egg counts per gram of feces (EPG) were performed. Coprocultures were prepared using 10 g of fecal material with the groups defined as follows: control group G1 (1.0 mL of denatured proteolytic crude extract); treated group G2 (1.0 mL of active proteolytic crude extract); treated group G3 (1.0 mL of active proteolytic crude extract + 1.0 mL of AC001 conidia). The coprocultures were maintained at room temperature (25ºC), for 7 days, and then the L3 larvae were recovered. The results demonstrated that AC001 successfully produced protease (56.34 U/mL). The treatments with active proteolytic crude extract (G2) and active proteolytic crude extract + AC001 conidia (G3) were significantly different (p < 0.01) from the control group with denatured proteolytic crude extract (G1). AC001 and its proteolytic crude extract acted concomitantly on helminths directly in the fecal environment, suggesting potential future applications in the field.


Assuntos
Ascomicetos , Fezes , Doenças dos Ovinos , Animais , Fezes/parasitologia , Fezes/microbiologia , Ovinos , Ascomicetos/fisiologia , Doenças dos Ovinos/parasitologia , Doenças dos Ovinos/terapia , Larva , Controle Biológico de Vetores/métodos , Proteólise , Peptídeo Hidrolases/metabolismo , Infecções por Nematoides/veterinária , Infecções por Nematoides/terapia , Contagem de Ovos de Parasitas/veterinária
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