RESUMO
Protein preparations of three S. pneumoniae strains belonging to different serological types according to the structure of their capsular polysaccharides have been analyzed. Immunoblotting with antiserum to a protein preparation isolated from one strain, pooled serum of patients with pneumococcal infection and individual sera of such patients have revealed that different S. pneumoniae strains have serologically active proteins with similar molecular weights. Analysis made with the use of rabbit antiserum to a protein preparation has demonstrated that most of such preparations (mol. wt. of 44, 49, 50 kD, etc.) belong to the group of proteins Pnp II, average in their molecular weight among the preparations under study (23-53 kD). At the same time serological response in pneumococcal infection is linked with common S. pneumoniae proteins with mol. wt. of 50, 87 and 97 kD, but not always with pneumolysin.
Assuntos
Antígenos de Bactérias/classificação , Proteínas de Bactérias/classificação , Streptococcus pneumoniae/imunologia , Adulto , Antígenos de Bactérias/análise , Antígenos de Bactérias/isolamento & purificação , Proteínas de Bactérias/análise , Proteínas de Bactérias/isolamento & purificação , Criança , Eletroforese em Gel de Poliacrilamida/métodos , Humanos , Immunoblotting , Peso Molecular , Infecções Pneumocócicas/microbiologia , Sorotipagem , Streptococcus pneumoniae/classificação , Streptococcus pneumoniae/isolamento & purificaçãoAssuntos
Anticorpos Antibacterianos/análise , Derrame Pleural/imunologia , Saliva/imunologia , Streptococcus pneumoniae/imunologia , Adolescente , Envelhecimento/imunologia , Formação de Anticorpos/imunologia , Proteínas de Bactérias/imunologia , Criança , Pré-Escolar , Humanos , Imunidade Inata/imunologia , Lactente , Pleurisia/imunologia , Pneumonia Pneumocócica/imunologia , Polissacarídeos Bacterianos/imunologiaRESUMO
Nonencapsulated strains obtained by subculturing Streptococcus pneumoniae in a medium containing 0.5% of bile have been shown to possess a number of morphological and cultural features. The preparations obtained from the biomass of these strains by sonication have been found to possess antigenic, immunogenic and protective properties and to be specific to S. pneumoniae. The data obtained in this investigation may be considered indicative of the necessity for further study of these preparations with a view to their use for the analysis of serum samples taken from patients with bronchopulmonary diseases by means of enzyme immunoassay.
