Assuntos
Choque Séptico/etiologia , Infecções Estreptocócicas/etiologia , Streptococcus pyogenes/patogenicidade , Anticorpos Antibacterianos/sangue , Antiestreptolisina/sangue , Bacteriemia/etiologia , Desoxirribonucleases/imunologia , Humanos , Insuficiência de Múltiplos Órgãos/etiologia , Choque Séptico/diagnóstico , Choque Séptico/microbiologia , Infecções Estreptocócicas/diagnóstico , Infecções Estreptocócicas/microbiologia , Streptococcus pyogenes/imunologia , Streptococcus pyogenes/isolamento & purificaçãoAssuntos
Bioprótese/efeitos adversos , Endocardite Bacteriana/diagnóstico por imagem , Infecções por Bactérias Gram-Negativas/diagnóstico por imagem , Próteses Valvulares Cardíacas/efeitos adversos , Infecções Relacionadas à Prótese/diagnóstico por imagem , Veillonella , Ecocardiografia , Endocardite Bacteriana/etiologia , Infecções por Bactérias Gram-Negativas/etiologia , Humanos , Masculino , Pessoa de Meia-Idade , Valva Mitral/diagnóstico por imagem , Infecções Relacionadas à Prótese/etiologiaRESUMO
Herpetic ocular disease never produces well-identifiable morphological aspects. It is expensive, complicated and slow to culture Herpes simplex virus. The validity of the fluorescent monoclonal antibodies test was investigated using corneal cells with or without a sure herpetic lesion. The tests, on 36 cases without sure herpetic lesions, were always negative, while of the 42 patients with a clinically ascertained herpetic lesion, 38 were positive (90.5%) and 4 negative (9.5%), two of these on account of inadequate tissue preparations. Thus, this method can prove useful and reliable for diagnostic purposes.
Assuntos
Imunofluorescência , Ceratite Herpética/diagnóstico , Anticorpos Antivirais/análise , Córnea/microbiologia , Lesões da Córnea , Corpos Estranhos no Olho/diagnóstico , Ferimentos Oculares Penetrantes/diagnóstico , Humanos , Microscopia de Fluorescência , Reprodutibilidade dos Testes , Simplexvirus/imunologiaRESUMO
In the autumn of 1983, an outbreak of recurrent abdominal cramps occurred in a nursery and primary school in the Rovigo area in Italy. None of the 10 affected children had diarrhea. An atypical Campylobacter-like organism was isolated from feces in all cases. Conventional enteropathogens were searched for but not detected. The Campylobacter-like organism was identified as Arcobacter butzleri by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins and cellular fatty acid analysis. Its identity was confirmed by DNA-DNA hybridizations versus Arcobacter reference strains. All of the preserved outbreak strains have identical protein profiles and phenotypic characteristics and belong to serogroup 1 of the Lior serotyping scheme on the basis of slide agglutination of crude and absorbed antisera of A. butzleri reference strains versus heat-labile antigens of live bacteria. These data point to an epidemiological relationship. The successive timing of the cases suggests person-to-person transmission.
Assuntos
Infecções por Campylobacter/microbiologia , Campylobacter/patogenicidade , Surtos de Doenças , Gastroenteropatias/microbiologia , Campylobacter/classificação , Campylobacter/isolamento & purificação , Infecções por Campylobacter/epidemiologia , Criança , Pré-Escolar , Fezes/microbiologia , Feminino , Humanos , Itália/epidemiologia , Masculino , Fenótipo , VirulênciaAssuntos
Staphylococcus aureus/efeitos dos fármacos , Staphylococcus epidermidis/efeitos dos fármacos , Aminoglicosídeos , Antibacterianos/farmacologia , Coagulase/análise , Resistência Microbiana a Medicamentos , Humanos , Incidência , Resistência a Meticilina , Estudos Retrospectivos , Staphylococcus aureus/enzimologia , Staphylococcus aureus/isolamento & purificação , Staphylococcus epidermidis/enzimologia , Staphylococcus epidermidis/isolamento & purificaçãoRESUMO
One hundred and fifty five strains of Yersinia sp. isolated in Italy from 1981 through 1983 were studied for their ability to utilize esculin, D-lixose, L-fucose and D-arabitol. All the strains belonging to biotype 4 serotype 3 isolated from humans failed to ferment such carbohydrates. The strains of other bioserotypes showed different biochemical reactions.
Assuntos
Yersinia/metabolismo , Animais , Esculina/metabolismo , Fermentação , Fucose/metabolismo , Humanos , Álcoois Açúcares/metabolismo , Yersinia/classificaçãoRESUMO
665 samples of milk, 20 of bovine faeces, 7 of human faeces of cowherd and some water samples are examined. 44 strains of Yersinia sp. are isolated of which 16 from patients and carriers in the same area. Strains belonging to the same bioserotype have been isolated from more than one source. Possibility of reciprocal contamination is discussed.
