RESUMO
We have identified 149 hybridization probes at 10-cM intervals in the mouse and have confirmed their order and linkage by fluorescence in situ hybridization. These probes represent a new resource for mapping in the mouse and can be used to correlate linkage and cytogenetic maps, to map novel sequences to within a few centimorgans, to relate cytogenetic abnormalities to the genetic map, and to make cross-species comparisons.
Assuntos
Sondas de DNA/genética , Ligação Genética/genética , Marcadores Genéticos/genética , Hibridização in Situ Fluorescente , Mapeamento Físico do Cromossomo , Animais , Aberrações Cromossômicas/genética , Cromossomos/genética , Cromossomos Artificiais de Levedura/genética , Clonagem Molecular , Corantes Fluorescentes , Camundongos , Reação em Cadeia da Polimerase , Recombinação Genética/genética , Reprodutibilidade dos Testes , Especificidade da EspécieRESUMO
We have developed a method, which we have named hybridization-banding, to identify simultaneously all chromosomes in a mouse metaphase spread. The method uses a combination of hybridization probes labeled with a single fluor to yield a simple, unique, readily identifiable hybridization pattern on each chromosome. The method is superior to Giemsa- or fluorescence-based banding methods for chromosome identification because the hybridization patterns are simpler and easier to identify, and unique patterns can be designed at will for each chromosome. Analysis can be performed with a standard fluorescence microscope, and images can be recorded on film with an ordinary 35-mm camera, making the method useful to many investigators. The method can also be applied to any species for which chromosomes and probes can be prepared.
