3-Cyclopropyl-1,2,4-triazolo[3,4-b]benzothiazole monohydrate.

The title structure, C11H9N3S.H2O, contains two crystallographically independent molecules, each consisting of a 1,2,4-triazolo[3,4-b]benzothiazole-fused ring fragment substituted with a cyclopropyl ring, and a water molecule. The geometry of both molecules differs slightly. Both molecules are planar within 0.041 and 0.03 A. The dihedral angle between triazole and the cyclopropyl ring is 60.0(1) and 61.7(1) degrees for molecules A and B, respectively, and the cyclopropyl ring is inclined at an angle of 2.0(1) and 1.2(1) degrees to the benzothiazole moiety for molecules A and B, respectively. The packing of the molecules is stabilized by O-H...N-type hydrogen bonds.

Involvement of nitric oxide and nitric oxide synthase activity in anticonvulsive action.

The anticonvulsant drug Diazepam (DIA-2 mg/kg b. wt), the nitric oxide (NO) donor L-Arginine (L-Arg-2000 mg/kg b. wt) and the putative nitric oxide synthase (NOS) inhibitor N(G)-Nitro-L-Arginine methyl ester (L-NAME-50 mg/kg b. wt) were used to determine the role of endogenous NO on convulsions induced by picrotoxin (PCT-5 mg/kg b. wt) in rats. Rats given a convulsant dose of PCT (5 mg/kg b. wt) had convulsion and it suppresses the NOS activity and NO concentration in brain regions. The anticonvulsant L-Arg alone significantly increases the NO concentration and NOS activity in brain regions, but not diazepam. Whereas DIA, along with L-Arg, enhances the NO and NOS activity when compared to L-Arg alone. The combination of both OIA and L-Arg completely suppressed the convulsions. L-NAME alone had no effect to produce convulsions but it completely decreased NO concentration and NOS activity and potentiated the PCT convulsions. This was reverted by pre- and post treatment of DIA plus L-Arg indicating, the increased NO concentration and NOS activity in brain regions suppresses convulsions.